Metagenomics of wheat rhizosphere for abiotic stress management

Ahlawat, Om Parkash; Tiwari, Ruchi; Singh, Gyanendra Pratap

doi:10.25174/2249-4065/2018/79291

Cited by 7 publications

(7 citation statements)

References 42 publications

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“…The evolution of bacterial communities in rhizospheric samples from wheat fields was studied by Donn et al (2015). Ahlawat et al (2018) deduced the eventual significance of rhizospheric bacteria to combat stress conditions by studying the metagenomics of wheat rhizosphere. Germida and Siciliano (2001) have shown that the taxonomic diversity of bacteria congregated to the roots of modern, recent, and ancient cultivars of wheat.…”

Section: Discussionmentioning

confidence: 99%

Seed endophytic bacterial profiling from wheat varieties of contrasting heat sensitivity

et al. 2023

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Wheat yield can be limited by many biotic and abiotic factors. Heat stress at the grain filling stage is a factor that reduces wheat production tremendously. The potential role of endophytic microorganisms in mitigating plant stress through various biomolecules like enzymes and growth hormones and also by improving plant nutrition has led to a more in-depth exploration of the plant microbiome for such functions. Hence, we devised this study to investigate the abundance and diversity of wheat seed endophytic bacteria (WSEB) from heatS (heat susceptible, GW322) and heatT (heat tolerant, HD3298 and HD3271) varieties by culturable and unculturable approaches. The results evidenced that the culturable diversity was higher in the heatS variety than in the heatT variety and Bacillus was found to be dominant among the 10 different bacterial genera identified. Though the WSEB population was higher in the heatS variety, a greater number of isolates from the heatT variety showed tolerance to higher temperatures (up to 55°C) along with PGP activities such as indole acetic acid (IAA) production and nutrient acquisition. Additionally, the metagenomic analysis of seed microbiota unveiled higher bacterial diversity, with a predominance of the phyla Proteobacteria covering >50% of OTUs, followed by Firmicutes and Actinobacteria. There were considerable variations in the abundance and diversity between heat sensitivity contrasting varieties, where notably more thermophilic bacterial OTUs were observed in the heatT samples, which could be attributed to conferring tolerance against heat stress. Furthermore, exploring the functional characteristics of culturable and unculturable microbiomes would provide more comprehensive information on improving plant growth and productivity for sustainable agriculture.

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Section: Discussionmentioning

confidence: 99%

Seed endophytic bacterial profiling from wheat varieties of contrasting heat sensitivity

et al. 2023

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“…It is quite possible to culture only very limited numbers of microorganisms and analysis of them for beneficial traits by using cultural methodology. However, techniques like metagenomics are very tough in analytical study of whole community interaction and coordination between different components of the community and metabolic pathways or genes activity under particular to relevant environmental condition with own specificity [20] [21].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Deciphering Fungal Community structure in Zea mays L. and Triticum Aestivum L

Hussain

Waseem

Mumtaz

et al. 2022

IJIST

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Rhizosphere fungi are strongly associated with plant growth and health by providing nutrients and antagonizing pathogens. Commercially, fungus has multipurpose applications in several sectors including beverages, food items and in medicines. Current study aimed to reveal the core fungal community structure of the two leading cereal crops that are Zea mays L. and Triticum aestivum L. The rhizosphere fungal community was explored via morphology, biochemistry and internal transcribe spacer (ITS) metagenomics. On the basis of morphology, the retrieved fungal strains were imprecisely classified into Ascomycota and Zygomycota. The species including Yeast, Botyritis californica, Rhizopus stolonifer, Alternaria tenuissima, Aspergillus terreus, Aspergillus flavus, Aspergillus nidulans, Aspergillus niger and Microsporum canis were identified on the basis of macroscopy and microscope. Moreover, the biochemical characterization depicted the role of fungi in promotion of plant growth. Majority of the isolates depicted catalase activity, indole production, phosphate solubilization, ammonia production, nitrogenase activity and urease activity. Metagenomics using amplicon sequencing of ITS region revealed the presence of 805 Operational Taxonomic Units (OTUs) with 647 OTUs in Zea mays and 620 OTUs in Triticum aestivum. The fungal phyla found in the rhizosphere of Zea mays L. and Triticum aestivum L. were Ascomycota, Basidiomycota, Zygomycota, Chytridiomycota, Incertae sedis fungi. Ascomycota accounted for 93% and 95% of classified fungi in rhizosphere of Zea mays L. and Triticum aestivum L. respectively. The dominant species found in the rhizosphere soil of Zea mays were Gibberella intricans, Curvularia lunata, Lepidosphaeria nicotiae, Edenia gomezpompae and Myrothecium verrucaria.

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“… Zolla et al (2013) , work on drought stress conditions in Arabidopsis thaliana using the pyrosequencing approach reported the robust set of soil microbes having an ability to sense abiotic stress and increase plant biomass production along with reduced expression of drought response marker genes ( Zolla et al, 2013 ). A similar study on wheat rhizosphere for abiotic stress management reported the wider and diversified role of metagenomics in ASM where metagenomics of wheat along with its application in climate-resilient genotypes development is elaborated ( Ahlawat et al, 2018 ).…”

Section: Metagenomics and Sustainable Agriculturementioning

confidence: 99%

Potential Use of Microbial Community Genomes in Various Dimensions of Agriculture Productivity and Its Management: A Review

et al. 2022

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Agricultural productivity is highly influenced by its associated microbial community. With advancements in omics technology, metagenomics is known to play a vital role in microbial world studies by unlocking the uncultured microbial populations present in the environment. Metagenomics is a diagnostic tool to target unique signature loci of plant and animal pathogens as well as beneficial microorganisms from samples. Here, we reviewed various aspects of metagenomics from experimental methods to techniques used for sequencing, as well as diversified computational resources, including databases and software tools. Exhaustive focus and study are conducted on the application of metagenomics in agriculture, deciphering various areas, including pathogen and plant disease identification, disease resistance breeding, plant pest control, weed management, abiotic stress management, post-harvest management, discoveries in agriculture, source of novel molecules/compounds, biosurfactants and natural product, identification of biosynthetic molecules, use in genetically modified crops, and antibiotic-resistant genes. Metagenomics-wide association studies study in agriculture on crop productivity rates, intercropping analysis, and agronomic field is analyzed. This article is the first of its comprehensive study and prospects from an agriculture perspective, focusing on a wider range of applications of metagenomics and its association studies.

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Metagenomics of wheat rhizosphere for abiotic stress management

Cited by 7 publications

References 42 publications

Seed endophytic bacterial profiling from wheat varieties of contrasting heat sensitivity

Seed endophytic bacterial profiling from wheat varieties of contrasting heat sensitivity

Molecular characterization of Deciphering Fungal Community structure in Zea mays L. and Triticum Aestivum L

Potential Use of Microbial Community Genomes in Various Dimensions of Agriculture Productivity and Its Management: A Review

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