Metahyphopichia laotica gen. nov., sp. nov., a polymorphic yeast related to Hyphopichia

Sipiczki, Matthias; Pfliegler, Walter P.; Safar, Silvana V. B.; Morais, Paula B.; Rosa, Carlos A.

doi:10.1099/ijsem.0.001091

Cited by 5 publications

(9 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results were consistent with the multilocus phylogenetic analysis reported by Sipiczki et al . [13], showing the placement of C. silvanorum close to M. laotica , which was supported by a high posterior probability value, and this being a monophyletic clade. Thus, this evidence was reliable enough to place C. silvanorum in the genus Metahypopichia .…”

Section: Novel Species Identification and Delineationmentioning

confidence: 84%

“…nov.; 3, Metahyphopichia laotica. Data for species 1 is from this study; for species 2 is from Lachance et al[34] and for species 3 from Sipiczki et al[13]. Growth reactions: −, no growth/fermentation; +, strong growth/fermentation; w, weak growth/fermentation; s, slow growth/fermentation; nd, not determined; +/s, strong growth or slow growth.Metahyphopichia suwanaadthiae ( su.…”

mentioning

confidence: 90%

“…Based on the SSU rRNA gene sequences, Suzuki and Nakase [12] suggested that C. silvanorum had a relationship with C. entomophila and other Candida species that showed affinities to H. burtonii. The genus Metahypopichia, a single-species genus, was described by Sipiczki et al [13] with Metahypopichia laotica as the type species. The genus was proposed from six strains comprising four strains isolated from flowers in Laos and two strains obtained from bark samples collected in Brazil.…”

Section: Introductionmentioning

confidence: 99%

“…The genus was proposed from six strains comprising four strains isolated from flowers in Laos and two strains obtained from bark samples collected in Brazil. In phylogenetic analysis of a multilocus sequence comprising the SSU rRNA gene, the D1/D2 domains of the LSU rRNA gene, actin ( ACT1 ) and TEF1 genes, revealed that M. laotica was closest to C. silvanorum with high posterior probability support and appeared to be a monophyletic clade connected to the genera Hyphopichia and Danielozyma [13].…”

Section: Introductionmentioning

confidence: 99%

“…The genus Metahypopichia , a single-species genus, was described by Sipiczki et al . [13] with Metahypopichia laotica as the type species. The genus was proposed from six strains comprising four strains isolated from flowers in Laos and two strains obtained from bark samples collected in Brazil.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Metahyphopichia suwanaadthiae sp. nov., an anamorphic yeast species in the order Saccharomycetales and reassignment of Candida silvanorum to the genus Metahyphopichia

Khunnamwong

Savarajara

Jindamorakot

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Seven yeast strains, representing a single novel anamorphic species, were isolated in Thailand. They consisted of five strains (DMKU-MRY16T, DMKU-SK18, DMKU-SK25, DMKU-SK30 and DMKU-SK32) obtained from five different mushrooms, and two strains (ST-224 and 11-14.2) derived from insect frass and soil, respectively. The pairwise sequence analysis indicated that all seven strains had identical sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. Metahyphopichia silvanorum was the most closely related species, but with 11.9–12.4% nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 13.1–13.3% nucleotide substitutions in the ITS region. The phylogenetic analyses based on the concatenated sequences of the ITS region and the D1/D2 domains of the LSU rRNA gene showed that the seven strains form a well-separated subclade in a clade containing M. silvanorum and Metahyphopichia laotica with high bootstrap support. A phylogenetic analysis of a multilocus dataset including the small subunit (SSU) rRNA gene, the ITS region, the D1/D2 domains of the LSU rRNA gene, translation elongation factor 1-alpha gene, actin gene and the RNA polymerase II subunit 2 gene, confirmed the presence of the monophyletic clade that also includes M. silvanorum and M. laotica, and strongly supported the phylogenetic isolation of the seven strains from its neighbouring species. Therefore, the seven strains were assigned as a single novel species of the genus Metahyphopichia, according to their phylogenetic relationships. The name Metahyphopichia suwanaadthiae sp. nov. is proposed to accommodate the seven strains. The holotype is DMKU-MRY16T (TBRC 11775T=NBRC 114386T=PYCC 8655T). The MycoBank number of the novel species is MB 841280. In addition, Candida silvanorum is reassigned to the genus Metahyphopichia. The MycoBank number of M. silvanorum comb. nov. is MB 841279.

show abstract

Section: Novel Species Identification and Delineationmentioning

confidence: 84%

mentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Metahyphopichia suwanaadthiae sp. nov., an anamorphic yeast species in the order Saccharomycetales and reassignment of Candida silvanorum to the genus Metahyphopichia

Khunnamwong

Savarajara

Jindamorakot

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation

Rosa,

Lachance,

Limtong

et al. 2023

Yeast

View full text Add to dashboard Cite

Tropical rainforests and related biomes are found in Asia, Australia, Africa, Central and South America, Mexico, and many Pacific Islands. These biomes encompass less than 20% of Earth's terrestrial area, may contain about 50% of the planet's biodiversity, and are endangered regions vulnerable to deforestation. Tropical rainforests have a great diversity of substrates that can be colonized by yeasts. These unicellular fungi contribute to the recycling of organic matter, may serve as a food source for other organisms, or have ecological interactions that benefit or harm plants, animals, and other fungi. In this review, we summarize the most important studies of yeast biodiversity carried out in these biomes, as well as new data, and discuss the ecology of yeast genera frequently isolated from tropical forests and the potential of these microorganisms as a source of bioinnovation. We show that tropical forest biomes represent a tremendous source of new yeast species. Although many studies, most using culture‐dependent methods, have already been carried out in Central America, South America, and Asia, the tropical forest biomes of Africa and Australasia remain an underexplored source of novel yeasts. We hope that this review will encourage new researchers to study yeasts in unexplored tropical forest habitats.

show abstract

Taxogenomic placement of Rasporella oleae and Rasporella dianae gen. and spp. nov., two insect associated yeast species

Čadež,

Boundy‐Mills,

Botha

et al. 2023

Yeast

View full text Add to dashboard Cite

During the course of independent studies in Europe, North America, and Africa, seven yeast strains were isolated from insect frass, decaying wood, tree flux, and olive oil sediment. Phylogenetic analysis of two barcoding DNA regions (internal transcribed spacer and the D1/D2 domain of the LSU rRNA gene) revealed that they belong to two closely related undescribed species distinct from all genera in the family Debaryomycetaceae. For reliable taxonomic placement the genomes of four strains of the two novel species and six type strains of closely related species were sequenced. Orthologous genes from 54 genomes of representatives of the Pichiomycetes and 23 outgroup taxa were concatenated to construct a fully supported phylogenetic tree. Consistent with the assumptions, we found that the two new species belong to a novel genus. In addition, the delimitation of the novel species was supported by genetic distance calculations from average nucleotide identity (ANI) and digital DNA:DNA hybridization (dDDH) values. The physiological characterization of the novel species was generally consistent with their genomic content. All strains had two alleles encoding secretory lipase in either two or three copies depending on the species. However, lipolytic activity was detected only in strains with three copies of the secretory lipase gene. Nevertheless, lipolytic activity might be related to their association with the insect gut. Based on these results, formal descriptions of the new genus Rasporella gen. nov. and of two new species Rasporella dianae sp. nov. (holotype UCDFST 68‐643T, MycoBank no.: 850238) and Rasporella oleae sp. nov. (holotype ZIM 2471T, MycoBank no.: 850126) are provided.

show abstract

Metahyphopichia laotica gen. nov., sp. nov., a polymorphic yeast related to Hyphopichia

Cited by 5 publications

References 43 publications

Metahyphopichia suwanaadthiae sp. nov., an anamorphic yeast species in the order Saccharomycetales and reassignment of Candida silvanorum to the genus Metahyphopichia

Metahyphopichia suwanaadthiae sp. nov., an anamorphic yeast species in the order Saccharomycetales and reassignment of Candida silvanorum to the genus Metahyphopichia

Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation

Taxogenomic placement of Rasporella oleae and Rasporella dianae gen. and spp. nov., two insect associated yeast species

Contact Info

Product

Resources

About