Metal and metallothionein distribution in different tissues of the Mediterranean clam Venerupis philippinarum during copper treatment and detoxification

Santovito, Gianfranco; Boldrin, Francesco; Irato, Paola

doi:10.1016/j.cbpc.2015.06.008

Cited by 24 publications

(22 citation statements)

References 63 publications

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“…Virtually all eukaryotic cells contain Cu,Zn SODs [37][38][39][40][41][42], as well as other anti-stress proteins that allow species to withstand environmental stresses of both natural and anthropogenic origin [43][44][45][46][47][48][49][50][51][52][53]. Our results indicated that a gene coding for cytosolic Cu,Zn SOD is present in the genome of the Antarctic icefish C. hamatus and is expressed as active protein in various organs.…”

Section: Discussionmentioning

confidence: 86%

Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

et al. 2020

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In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the “unclock-like” behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the regulation of gene expression related to environmental stress conditions and also animal physiology. The data presented are the first on the antioxidant enzymes of a fish belonging to the Channichthyidae family and represent an important starting point in understanding the antioxidant systems of these organisms that are subject to constant risk of oxidative stress.

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Section: Discussionmentioning

confidence: 86%

Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

et al. 2020

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“…Copper is found in CuZnSOD active sites and is involved in a variety of important biological processes; however, it could be toxic to organisms if its concentration exceeds the supraoptimal levels . Our recent study showed that CuCl 2 is highly toxic to C. ehrenbergii .…”

Section: Resultsmentioning

confidence: 93%

Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Wang

2019

Environmental Toxicology

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Superoxide dismutase (SOD) acts as the first line of defense against reactive oxygen species (ROS) within cells. In the present study, we determined two novel CuZnSOD genes (designated as CeCSD1 and CeCSD2) from the toxicity-testing freshwater algae Closterium ehrenbergii and examined their structural features, phylogenetic relationships, and gene expression under exposure to different metals. Putative CeCSD1 (204 aa, 20.6 kDa) and CeCSD2 (155 aa, 15.3 kDa) proteins had conserved CuZnSOD family motifs and metal (Cu, Zn) binding sites, but different N-terminus structures, that is, CeCSD1 has a signal peptide to chloroplasts. Phylogenetic analysis of each protein revealed that C. ehrenbergii was well clustered with other green algae and plants. Real-time PCR results showed that the gene expression obviously increased with heavy metal exposure. In addition, excess copper considerably increased the SOD activity and ROS generation but decreased the photosynthetic efficiency in treated cells. These results suggest that CeCSDs are involved in the antioxidant defense system and can be regarded as potential biomarkers for monitoring metal contaminants in aquatic environments. K E Y W O R D SCeCSD, Closterium ehrenbergii, gene expression, heavy metals, ROS

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“…It has been reported that the exposure of aquatic organisms to metals induced MT expression. Numerous studies have shown the bioaccumulation capacities of aquatic species such as rare minnow [28], mudskipper [29], Mediterranean clam [30], and blue crab [31]. MT genes have also been reported in various plant species, such as Arabidopsis thaliana [32], Olea europaea L. [33], and…”

Section: Metal Tolerance and Ion Accumulation In E Coli To Assess Tmentioning

confidence: 99%

Type 1 metallothionein (ZjMT) is responsible for heavy metal tolerance in Ziziphus jujuba

Lansong

Meng

Cao

et al. 2016

Biochemistry Moscow

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Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of Ziziphus jujuba. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in Z. jujuba within 24 h upon Cd exposure. Escherichia coli cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and E. coli cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater.

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Metal and metallothionein distribution in different tissues of the Mediterranean clam Venerupis philippinarum during copper treatment and detoxification

Cited by 24 publications

References 63 publications

Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Type 1 metallothionein (ZjMT) is responsible for heavy metal tolerance in Ziziphus jujuba

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