Metal-binding properties of phytochelatin-related peptides

Satofuka, Hiroyuki; Fukui, Toshiaki; Takagi, Masahiro; Atomi, Haruyuki; Imanaka, Tadayuki

doi:10.1016/s0162-0134(01)00223-9

Cited by 49 publications

(21 citation statements)

References 25 publications

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“…In wheat, phytochelatins bound 82% of the Cd in roots, 19% in young leaves and 12% in old leaves, suggesting the speciality of PC-based Cd sequesteration varies with tissues even in the same plant (Marentes & Rauser, 2007). And it's demonstrated that the chemical structure of thiol and carboxyl groups is essential for the metal-binding ability and formation of a Cd-PCs complex (Satofuka et al, 2001). There are two types of Cd-PC complexes produced during Cd sequesteration: lowmolecular-weight (LMW) and high-molecular-weight (HMW).…”

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confidence: 99%

Towards Understanding Plant Response to Heavy Metal Stress

Zhao¹,

Chu²

2011

Abiotic Stress in Plants - Mechanisms and Adaptations

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IntroductionMetals like zinc, iron and copper are essential micronutrients required for a wide range of physiological processes in all plant organs for the activities of various metal-dependent enzymes and proteins. However, they can also be toxic at elevated levels. Metals like arsenic, mercury, cadmium and lead are nonessential and potentially highly toxic. Once the cytosolic metal concentration in plant turns out of control, phytotoxicity of heavy metal inhibits transpiration and photosynthesis, disturbs carbohydrate metabolism, and drives the secondary stresses like nutrition stress and oxidative stress, which collectively affect the plant development and growth (Krämer & Clemens, 2005). Plants have developed a complex network of highly effective homeostatic mechanisms that serve to control the uptake, accumulation, trafficking, and detoxification of metals. Components of this network have been identified continuously, including metal transporters in charge of metal uptake and vacuolar transport; chelators involved in metal detoxification via buffering the cytosolic metal concentrations; and chaperones helping delivery and trafficking of metal ions (Clemens, 2001). This chapter summarizes heavy metal stress and detoxification in plant. Special focus is given to metallothionein, yet vacuolar metal transporters, phytochelatins as well as certain organic acids, amino acids, and chaperones are also addressed with recent advances. Besides, heavy metal-induced oxidative stress and tolerance as an example of abiotic stress cross-talk will be discussed.

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confidence: 99%

Towards Understanding Plant Response to Heavy Metal Stress

Zhao¹,

Chu²

2011

Abiotic Stress in Plants - Mechanisms and Adaptations

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“…PCs have the common structure (␥-GluCys) n Gly, where n ϭ 2 Ϫ 11, although PC 2 and PC 3 are the most common (Cobbett, 2000;Cobbett and Goldsbrough, 2002). There is strong evidence that PCs complex metal(loid) ions, for which they have high affinity, in plant extracts (Mehra et al, 1996a(Mehra et al, , 1996bBae and Mehra, 1997;Leopold and Gunther, 1997;Leopold et al, 1998;Pickering et al, 1999;Satofuka et al, 2001;Cruz et al, 2002;Doreak and Krezel, 2003).…”

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The Nature of Arsenic-Phytochelatin Complexes in Holcus lanatus and Pteris cretica

2004

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(A.A.M.).We have developed a method to extract and separate phytochelatins (PCs)-metal(loid) complexes using parallel metal(loid)-specific (inductively coupled plasma-mass spectrometry) and organic-specific (electrospray ionization-mass spectrometry) detection systems-and use it here to ascertain the nature of arsenic (As)-PC complexes in plant extracts. This study is the first unequivocal report, to our knowledge, of PC complex coordination chemistry in plant extracts for any metal or metalloid ion. The As-tolerant grass Holcus lanatus and the As hyperaccumulator Pteris cretica were used as model plants.In an in vitro experiment using a mixture of reduced glutathione (GS), PC 2 , and PC 3 , As preferred the formation of the arsenite [As (III) ]-PC 3 complex over GS-As (III) -PC 2 , As (III) -(GS) 3 , As (III) -PC 2 , or As (III) -(PC 2 ) 2 (GS: glutathione bound to arsenic via sulphur of cysteine). In H. lanatus, the As (III) -PC 3 complex was the dominant complex, although reduced glutathione, PC 2 , and PC 3 were found in the extract. P. cretica only synthesizes PC 2 and forms dominantly the GS-As (III) -PC 2 complex. This is the first evidence, to our knowledge, for the existence of mixed glutathione-PC-metal(loid) complexes in plant tissues or in vitro. In both plant species, As is dominantly in non-bound inorganic forms, with 13% being present in PC complexes for H. lanatus and 1% in P. cretica.Phytochelatins (PCs) are induced in a wide range of plant species by the oxy-anions arsenate [As (V) ] and selenate and a range of cations such as Ag ϩ , Cd 2ϩ , Cu 2ϩ , Hg 2ϩ , and Pb 2ϩ (Grill et al., 1985). They are synthesized from reduced glutathione (GSH) by the transpeptidation of ␥-glutamyl-cysteinyl dipeptides through the action of the constitutive enzyme PC synthase (Schmö ger et al., 2000;Vatamaniuk et al., 2000). PCs have the common structure (␥-GluCys) n Gly, where n ϭ 2 Ϫ 11, although PC 2 and PC 3 are the most common (Cobbett, 2000; Cobbett and Goldsbrough, 2002). There is strong evidence that PCs complex metal(loid) ions, for which they have high affinity, in plant extracts (Mehra et al., 1996a(Mehra et al., , 1996b Bae and Mehra, 1997;Leopold and Gunther, 1997;Leopold et al., 1998;Pickering et al., 1999;Satofuka et al., 2001; Cruz et al., 2002; Doreak and Krezel, 2003).Little is known of the nature of metal(loid) ion-PC complexes in planta. Preliminary experiments with cadmium and copper using HPLC using chromatography with metal-specific detectors (coupled to inductively coupled plasma [ICP]-mass spectrometry[MS], UV detection, or off-line atomic absorption spectrometry; Maitani et al., 1996;Mehra et al., 1996a;Leopold and Gunther, 1997;Leopold et al., 1998;Scarano and Morelli, 2002) did not give convincing results for the complex formation. More basic preparative scale chromatography with fraction collectors have shown that presumed PC complexes have eluted from columns intact (Sneller et al., 1999;Schmö ger et al., 2000). Off-line analysis of PCs in collected fractions is performed by HPLC a...

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“…Since GSH was not utilized for PC synthesis, its enhanced accumulation must have constituted another advantage for plants, e.g., for direct metal chelation (metal inactivation) or for diminishing the deleterious effects of metals, such as oxidative stress. The latter seems more probable as GSH is regarded as a rather weak metal chelator (Satofuka et al 2001) but a key player in the cell antioxidative system and in maintenance of cellular redox homeostasis (Jozefczak et al 2012). Interestingly, both under the unpolluted and polluted soil conditions, higher GSH levels were found in the NM ecotype, supporting its role in basal metal tolerance but not in enhanced adaptive tolerance.…”

Section: Is Metal Tolerance Related To Metal Accumulation?mentioning

confidence: 97%

Physiological mechanisms of adaptation of Dianthus carthusianorum L. to growth on a Zn-Pb waste deposit - the case of chronic multi-metal and acute Zn stress

2015

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Aims This study investigates the response of metallicolous (M) and nonmetallicolous (NM) ecotypes of Dianthus carthusianorum L. to chronic multi-metal and acute Zn stress. Methods Plants were cultivated on the Zn-Pb waste heap substrate and under Zn excess in hydroponics. Growth parameters as well as accumulation of organic acids and thiol peptides were determined as a function of metal accumulation. Results When grown on the metalliferous substrate, the M plants showed less phytotoxicity symptoms, lower foliar metal (Zn, Pb, Cd) accumulation, higher malate and citrate but lower glutathione content than the NM plants. When exposed to Zn excess in hydroponics, the M ecotype was also more tolerant but accumulated more Zn in comparison with the NM ecotype, accompanied by greater malate and citrate concentrations in the shoots, which were however not affected by increasing Zn doses. No phytochelatins were detected under any experimental conditions. Conclusions Both constitutive and adaptive tolerance was found in D. carthusianorum. Under chronic metal stress, enhanced tolerance results from restricted metal uptake to the shoots and probably from detoxification by organic acids; however, under acute Zn stress it is not related to diminished metal uptake or organic acids. Glutathione and phytochelatins are not implicated in adaptive metal tolerance.

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Metal-binding properties of phytochelatin-related peptides

Cited by 49 publications

References 25 publications

Towards Understanding Plant Response to Heavy Metal Stress

Towards Understanding Plant Response to Heavy Metal Stress

The Nature of Arsenic-Phytochelatin Complexes in Holcus lanatus and Pteris cretica

Physiological mechanisms of adaptation of Dianthus carthusianorum L. to growth on a Zn-Pb waste deposit - the case of chronic multi-metal and acute Zn stress

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