Metal bioaccessibility, particle size distribution and polydispersity of playground dust in synthetic lysosomal fluids

Meza-Figueroa, Diana; Barboza‐Flores, M.; Romero, Francisco Martín; Acosta-Elías, Mónica; Hernández‐Mendiola, Ernesto; Maldonado-Escalante, Francisco; Pérez-Segura, Efrén; González-Grijalva, Belem; Meza-Montenegro, María Mercedes; García-Rico, Leticia; Navarro-Espinoza, Sofía; Santacruz-Gómez, Karla; Gallego-Hernández, Ana L.; Pedroza‐Montero, M.

doi:10.1016/j.scitotenv.2019.136481

Cited by 27 publications

(24 citation statements)

References 77 publications

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“…Seven (out of the 14) targeted elements displayed a statistically significant influence of particle size on bioaccessibility (Al, Ba, Co, Fe, Sb, Ti and V), and in each case, the bioaccessibility was higher in PMfine than in PMcoarse (Figure 3a). The simple 0.1 M AC solution used in this study does not include reagents commonly used in ALF such as glycine, citrate and tartrate, which may enhance extraction efficiency [44]. However, the key benefit of using a simple AC solution (especially for determining trace elements in lightly loaded filter samples) is minimizing matrix interferences.…”

Section: Total Element Concentrations In Fine Versus Coarse Dust Fracmentioning

confidence: 99%

Thoracic Fraction (PM10) of Resuspended Urban Dust: Geochemistry, Particle Size Distribution and Lung Bioaccessibility

et al. 2021

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A fluidized bed aerosol generator was connected to a 13-stage cascade impactor (nanoMOUDI) for the size fractionation of urban dust (<10 µm), followed by the gravimetric analysis of loaded PTFE filter samples. This method was used to characterize the PM10 (thoracic) fraction of road dust sampled from expressways, arterial roads and local roads in Toronto, Canada. The fine particle fractions (<1.8 µm) of all the studied samples accounted for 51–72% of the resuspended PM10 (by weight). Elemental analysis using ICP-MS and ICP-OES revealed an overall trend of element enrichment in the <1.8 µm fraction compared to the coarse fraction (1.8–10 µm) of the road dust. By contrast, archived house dust samples displayed the reverse trend for most elements. The lung bioaccessibility of target elements (Al, B, Ba, Co, Cr, Fe, La, Mn, Mo, Sb, Sr, Ti, V and Zn) was assessed for each road dust fraction using 0.1 M ammonium citrate (pH 4.4) to simulate intracellular fluid and Gamble solution (pH 7.2) to simulate interstitial lung fluid. The <1.8 µm fraction of local road dust displayed significantly higher bioaccessibility (p < 0.05) for Zn when using Gamble solution, and for seven out of the 14 target elements when using ammonium citrate. These results show the importance of characterizing the fine fraction of road dust.

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Section: Total Element Concentrations In Fine Versus Coarse Dust Fracmentioning

confidence: 99%

Thoracic Fraction (PM10) of Resuspended Urban Dust: Geochemistry, Particle Size Distribution and Lung Bioaccessibility

et al. 2021

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“…The fluids can be categorised according to the regions of the lung that they are mimicking. Gamble's and modified Gamble's (with dipalmitoylphosphatidylcholine (DPPC) surfactant and other components such as mucin [12]) solutions simulate neutral (pH 7.3) extracellular fluids, though formulations vary in their inclusion of certain amino acids such as glycine and cysteine [7,8,14,15], which are known to chelate with metals [8,15]. A variant of Gamble's solution, Hatch's solution, simulates the influence of the mucous component that is likely to be prevalent in the tracheobronchial region of the lungs, with the inclusion of protein and enzyme components [7].…”

Section: Introductionmentioning

confidence: 99%

“…The macrophage lysosomal fluids contain proteases and bacterial enzymes and are at a much lower pH than the extracellular lung fluids, typically pH 4.5 [14]. These conditions are simulated in artificial lysosomal fluids (ALFs) by the inclusion of glycine, citrate, pyruvate, tartrate, and lactate [7,14,15].…”

Section: Introductionmentioning

confidence: 99%

“…There are contrasting literature opinions as to whether the more conservative ALF should be used to assess bioaccessibility [14,15] or whether uncertainty about the proportion of time that a particle is resident in lysosomal fluid means that SLF is a more realistic extraction fluid [4]. The choice is important because bioaccessibility is generally much higher in ALF compared to SLF and, in some studies, reported to be as high as 100% for Pb, Cu and As [15]. For example, bioaccessibility of Pb in three standards, BCR 723, NIST 2710a and NIST 1648a, ranged from 51 to 76% in ALF compared to 7.1 to 11.9% in modified Gamble's solution [14].…”

Section: Introductionmentioning

confidence: 99%

“…Sources may also change, in terms of metal speciation, over time because of weathering; this is particularly relevant to particulates generated from mining [16][17][18] and smelting [19] wastes. Other factors that have been considered to affect bioaccessibility are dissolution kinetics [17], surface reactions and complexation [20,21], redox reactions [3], particle size and shape [15,19] and the solid to liquid ratio (S/L ratio) [8,14]. Of these factors, S/L ratio and the potential for surface complexation are likely to have the most significant influence on the % bioaccessibility.…”

Section: Introductionmentioning

confidence: 99%

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New Insights into Health Risk Assessments for Inhalational Exposure to Metal(loid)s: The Application of Aqueous Chemistry Modelling in Understanding Bioaccessibility from Airborne Particulate Matter

et al. 2021

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Aqueous modelling of chemical speciation in simulated lung fluid (SLF) enables a better understanding of the underlying chemical factors that influence metal(loid) inhalation bioaccessibility from airborne particulate matter. Such an approach can be used to supplement experimental techniques that are integral to the health risk assessment of metal(loid) exposure by inhalational routes. In this paper, we modelled the aqueous chemistry of airborne particulate-bound metal(loid)s (As, Cu, Mn, Pb and Zn) in a SLF based on Gamble’s solution (neutral pH). The modelling was performed using two software packages (Geochemist’s Workbench 14 and OLI Studio 9.5) and a total of five thermochemical databases (GWB Thermo, MINTEQ, PHREEQC, WATEQ4F and the default database for OLI Studio). Modelled results were compared with experimentally determined bioaccessibilities for the NIST 2710a standard reference material (SRM) and with literature-reported bioaccessibilities for NIST 1648a and BCR 038 SRMs. Whilst the models correctly describe the observed increase in bioaccessibility for more dilute solid/liquid extraction ratios, the performance of the models against the fractional bias of the mean (FBmean) and the normalised mean square error (NMSE) statistical metrics was generally outside the acceptance criteria. Findings from an analysis of the main aqueous chemical species predicted to be present in SLF indicate that carbonate and chloride complexes of Cu, Mn, Pb and Zn predominate, whilst free cations (for Cu, Mn and Zn) and hydroxides (for Cu) also play a role in solubilisation. Arsenic is not predicted to form significant complexes with the SLF components and is present in solution mainly as the HAsO42− ion and its conjugate acid, H2AsO4−. For modelled runs where glycine and citrate were present, significant increases in the bioavailability of Cu and Zn were predicted as a result of complexation with these ligands. An additional finding from our experimental bioaccessibility results for NIST 2710a was that the inclusion of the lung fluid surfactant dipalmitoylphosphatidylcholine (DPPC) in the SLF did not significantly affect the bioaccessibility. Our study provides useful insights into the likely aqueous- and solid-phase speciation of metal(loid)s in SLF and highlights that future developments in this area should consider the role of mineralogy and surface interactions.

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Characteristics and health risks of the inhalable fraction of metal additive manufacturing powders

Alijagic,

Wang,

Vallabani

et al. 2024

Nano Select

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Metal additive manufacturing (AM) is gaining traction but raises worker health concerns due to micron‐sized powders, including fine inhalable particles. This study explored particle and surface characteristics, electrochemical properties, metal release in artificial lysosomal fluid (ALF), and potential toxicity of virgin and sieved virgin Fe‐based powders, stainless steel (316L), Fe, and two tooling steels. Virgin particles ranged in size from 1 to 100 µm, while sieved particles were within the respirable size range (<5–10 µm). Surface oxide composition differed from bulk composition. The Fe powder showed low corrosion resistance and high metal release due to a lack of protective surface oxide. Sieved particles of 316L, Fe, and one tooling steel released more metals into ALF than virgin particles, with the opposite was observed for the other tooling steel. Sieved particles had no notable impact on cell viability or micronuclei formation in human bronchial epithelial cells. Inflammatory response in human macrophages was generally low, except for the Fe powder and one tooling steel, which induced increased interleukin‐8 (IL‐8/CXCL‐8) and monocyte chemoattractant protein‐1 (MCP‐1/CCL‐2) secretion. This study underscores distinctions between virgin and sieved Fe‐based powders and suggests relatively low acute toxicity.

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Metal bioaccessibility, particle size distribution and polydispersity of playground dust in synthetic lysosomal fluids

Cited by 27 publications

References 77 publications

Thoracic Fraction (PM10) of Resuspended Urban Dust: Geochemistry, Particle Size Distribution and Lung Bioaccessibility

Thoracic Fraction (PM10) of Resuspended Urban Dust: Geochemistry, Particle Size Distribution and Lung Bioaccessibility

New Insights into Health Risk Assessments for Inhalational Exposure to Metal(loid)s: The Application of Aqueous Chemistry Modelling in Understanding Bioaccessibility from Airborne Particulate Matter

Characteristics and health risks of the inhalable fraction of metal additive manufacturing powders

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