Metal-chelated cryogels for amyloglucosidase adsorption: application for continuous starch hydrolysis

Abstract

In the present work, a new metal-chelating platform was designed by using IDA as a chelating agent and Cu(II) as an affinity component for amyloglucosidase adsorption. Poly(AAm-GMA) cryogels were used as structural elements, while GMA monomer served reactive epoxy groups for IDA immobilization. Synthesized cryogels were characterized by FTIR, SEM and EDX studies. Pore diameter of the whole polymeric structure was 3-10 μm. Effects of medium pH, temperature, ionic strength along with amyloglucosidase concentration were also investigated for more effective amyloglucosidase adsorption and maximum adsorbed amount of amyloglucosidase was 2.93 mg g −1 cryogel by the optimum conditions. Reusability profile of the poly(AAm-GMA)-IDA-Cu 2+ cryogels was also studied and it was found that the synthesized cryogels could be used repeatedly for many times without any significant decrease on their adsorption capacity. Also continuous hydrolysis of starch by using immobilized form of amyloglucosidase in a column system was studied.

Preparation and Properties of Cryogel Based on Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)

“…Júnior et al have studied the Cu 2+ –IDA affinity cryogel-adsorbing immunoglobulin Y (IgY) and provided useful information of the maximum adsorption yield and the dissociation constants of IgY that was used in the simulation of purification process of immobilized metal affinity chromatography (IMAC). Evli et al immobilized amyloglucosidase onto the Cu 2+ –IDA affinity cryogels for the hydrolysis of starch. This immobilized metal affinity cryogels were highly compatible with aqueous environments and made the catalytic enzymatic reactions easy and fast.…”