Metal Chelating and Cupric Ion Reducing Antioxidant Capacities of Ammoides atlantica Aqueous Extract

Abstract

Reactive oxygen (ROS) and nitrogen species (RNS) are produced in all cells and play important roles in physiology. The loss of the redox balance, either by an increase of oxidant molecules ROS and RNS or by decreased antioxidant system activities cause a state of oxidative stress. Several studies are going on worldwide directed towards finding natural antioxidants of plant origin. Plants containing phenolic compounds have been reported to possess strong antioxidant activity. The objective of this study is to evaluate total polyphenols and flavonoids contents (TPC and TFC) as well as examine the in vitro antioxidative properties from aqueous extract of Ammoides atlantica (AqE). TPC was estimated utilizing Folin-Ciocalteu's reagent. TFC was evaluated utilizing the aluminum chloride method. The antioxidant properties were evaluated using metal chelating and cupric ion reducing antioxidant capacity (CUPRAC) assays. Indeed, results showed that the AqE is rich in polyphenols (141.74±0.44 µg gallic acid equivalents/ mg of dry weight), and flavonoids (61.87±6.7 µg quercetin equivalent/ mg dry weight). These phytochemical compounds possess significant antioxidant activities. The results showed that AqE exhibited a good Metal chelating activity with an IC50 of 36.57±4.73 µg/ mL. CUPRAC assay showed that AqE extract exhibited high cupric ion reducing antioxidant capacity with an A0.5 of 8.58±0.13 µg/mL. These findings provide evidence that AqE of Ammoides atlantica is a potential source of antioxidant which have many benefits towards human health.
 Keywords: Ammoides atlantica, aqueous extract, phenolic compounds, metal chelating and cupric ion reducing antioxidant capacity.

Cytotoxic Sesquiterpenoids from Ammoides atlantica Aerial Parts

“…In Algeria, the plant family Apiaceae consists of about 55 genera and 130 species. Among them, the genus Ammoides is represented only by two species, of which one, A. atlantica (Coss & Durieu) H. Wolff, is an endemic plant 1 traditionally used as an infusion to treat headache, fever, diarrhea, and vitiligo 2 and is also added as a spice in some recipes.…”

Further constituents from Ammoides atlantica (Coss. & Durieu) H. Wolff and their cytotoxic activity evaluation

“…Ammoides altantica (Coss. & Durieu) H.Wolff (Apiaceae) is a herbaceous plant endemic to Algeria, where it is consumed as a spice or used as an ethnobotanic remedy against headache, fever, and diarrhea 1 . Few chemical studies on the plant aerial parts are available in the literature, reporting the chemical composition of essential oil 2 and polar extracts, rich in flavonoids and phenolic acids 3 .…”