Metal Complex Conjugates of Antisense DNA Which Display Ribozyme-Like Activity

Magda, Darren; Wright, Meredith; Crofts, Shaun; Lin, and Alice; Sessler, Jonathan L.

doi:10.1021/ja9711223

Cited by 96 publications

(61 citation statements)

References 19 publications

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“…Conjugation of various catalysts (other lanthanide(III) complexes, multi-nuclear metal complexes, or organic molecular scissors in Fig. (2) and (3) with DNA oligomers was also effective for site-selective RNA scission [24][25][26][27][28][29][30][31][32][33][34].…”

Section: Covalent Artificial Ribonucleasesmentioning

confidence: 99%

Site-Selective Artificial Ribonucleases and their Applications

Kuzuya¹,

Komiyama

2007

COC

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Recent developments in artificial enzymes for site-selective scission of RNA have been reviewed, and their advantages over ribozymes are discussed. Most of previous reports involve covalent fixation of molecular scissors to sequence-recognizing moieties. However, non-covalent strategy, in which these two components are never covalently bound each other, has made remarkable progresses. When oligonucleotide bearing an acridine (site-selective activator) forms duplex with RNA substrate, the phosphodiester linkage of the RNA in front of the acridine is selectively activated and hydrolyzed by various metal ions (e.g., Lu(III). These non-covalent artificial ribonucleases are simple enough to be used for various more complicated systems. For example, tandem activator for clipping of desired fragment from long RNA substrate is obtained by attaching two acridines to an oligonucleotide. By analyzing the resultant RNA fragment with MALDI-TOFMS, the RNA substrate is accurately genotyped in terms of both single nucleotide polymorphisms (SNPs) and insertion/deletion polymorphisms (Indels).

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Section: Covalent Artificial Ribonucleasesmentioning

confidence: 99%

Site-Selective Artificial Ribonucleases and their Applications

Kuzuya¹,

Komiyama

2007

COC

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“…The DNA fragments generated can be simply reattached, indicating a considerable advantage of lanthanides over oxidative DNA cleaving agents (Baker et al, 1999;Canaple, Hüsken, Hall, & Häner, 2002;Espinosa et al, 2004;Loennberg, Suzuki, & Komiyama, 2008;Magda, Wright, Crofts, Lin, & Sessler, 1997). Especially, attention has been directed toward two rare earth cations, Tb 3+ and Eu 3+ , because of the suitable spectroscopic properties (long-lived and linelike emission) and their resonance energy levels overlap with the triplet energy states of nucleic acid ligands irradiated with ultraviolet light (Ci, Li, & Liu, 1995).…”

Section: Introductionmentioning

confidence: 99%

DNA interaction of europium(III) complex containing 2,2′-bipyridine and its antimicrobial activity

Jahani

Khorasani-Motlagh

Noroozifar

2015

Journal of Biomolecular Structure and Dynamics

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The interaction of native fish salmon DNA (FS-DNA) with [Eu(bpy)3Cl2(H2O)]Cl, where bpy is 2,2'-bipyridine, is studied at physiological pH in Tris-HCl buffer by spectroscopic methods, viscometric techniques as well as circular dichroism (CD). These experiments reveal that Eu(III) complex has interaction with FS-DNA. Moreover, binding constant and binding site size have been determined. The value of Kb has been defined 2.46 ± .02 × 10(5) M(-1). The thermodynamic parameters are calculated by Van't Hoff equation, the results show that the interaction of the complex with FS-DNA is an entropically driven phenomenon. CD spectroscopy followed by viscosity as well as fluorescence and UV--Vis measurements indicate that the complex interacts with FS-DNA via groove binding mode. Also, the synthesized Eu(III) complex has been screened for antimicrobial activities.

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“…The sequence and structure specific cleavage of RNA is an important challenge in the development of artificial metallonucleases [1][2][3][4][5][6][7][8][9][10]. As a step toward this goal, many research groups have focused on the study of metal ion complex binding to simple ribooligonucleotides [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%