Metal complexes as components of luminescent signalling systems

Silva, A.Prasanna de; Fox, David B.; Huxley, Allen J. M.; McClenaghan, Nathan D.; Roiron, Juliette

doi:10.1016/s0010-8545(98)00275-6

Cited by 76 publications

(32 citation statements)

References 34 publications

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“…152 Luminescence of the metal ion may be either enhanced or quenched by the presence of the analyte. 153 The former process was used in the design of the supramolecular potassium sensor depicted in Figure 7a and comprised of three units. 75 The central one is a terpyridine-based moiety for the complexation of the luminescent sensing ion, Eu(III); the outer macrocycles are good complexing agents for potassium, and they are connected to the terpyridine unit by a phenyl group acting as an electronic relay which conveys electronic density from the nitrogen lone pairs located on the monoazacrown-6 ethers toward the central unit.…”

Section: A Edifices Containing 4f-and S-block Ionsmentioning

confidence: 99%

Lanthanide-Containing Molecular and Supramolecular Polymetallic Functional Assemblies

2002

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Section: A Edifices Containing 4f-and S-block Ionsmentioning

confidence: 99%

Lanthanide-Containing Molecular and Supramolecular Polymetallic Functional Assemblies

2002

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“…Fluorescence has long been viewed as a powerful tool for the study of single components in a complex biological system [1][2][3][4][5][6][7]. There is lately a rapidly growing interest in the development of fluorescent probes with high selectivity for their cellular component-targets.…”

Section: Introductionmentioning

confidence: 99%

The Coumarin Moiety as Chromophore of Fluorescent Ion Indicators in Biological Systems

Katerinopoulos

2004

CPD

120

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Fluorescent probes have evolved into an extremely useful tool for the detection of ions in biological systems. The design of ion indicators is based in the proper choice of the ion chelating group as well as the chromophore moiety. The chromophores of choice should fulfill a number of requirements concerning the photostability of the group, the range of the excitation and emission wavelengths of the indicators, the Stokes shift, the fluorescence quantum yield, the excitation and/or emission wavelength shift upon coordination of the probe with its target ion, the lipophilicity of the indicators, and their possible cell toxicity. Coumarin and its analogues have been extensively used in ion detection by incorporation of the coumarin chromophore in the larger indicator framework. Coumarins fulfill all the aforementioned requirements since they are relatively photostable and their excitation and emission maxima, in many cases, are long enough to minimise "background" fluorescence of cellular components, tissues and biological fluids. They exhibit Stokes shifts large enough to avoid significant overlap of the excitation and emission spectra, their fluorescence quantum yields allow for ion detection at low indicator concentrations, and they can be introduced to cells either by microinjection or as membrane permeable derivatives without causing cell death. Synthetic approaches, aiming at the optimisation of indicator properties, have extended the conjugated coumarin system either by introduction of substituents or by expansion of the heterocyclic system. In this review, the basic rationale for the selection of the particular coumarin analogues is analysed, synthetic pathways leading to the desired structures are presented, and properties and relative advantages in the use of these probes are described.

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“…Long-lifetime species are becoming widely used in biomedical research. For example, sensors based on transition metal-ligand complexes [37][38][39][40] and on luminescent lanthanides [41][42][43] have been developed. Luminescent lanthanides have also been developed as MRI contrast agents to provide both magnetic resonance and optical signals [44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Real-time background suppression during frequency domain lifetime measurements

Heřman

Maliwal

Lakowicz

2002

Analytical Biochemistry

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We describe real time background suppression of autofluorescence from biological samples during frequency domain or phase modulation measurements of intensity decays. For these measurements the samples were excited with a train of light pulses with widths below 1 ps. The detector was gated off for a short time period of 10 to 40 ns during and shortly after the excitation pulse. The reference signal needed for the frequency domain measurement was provided by a long-lifetime reference fluorophore which continues to emit following the off-gating pulse. Both the sample and the reference were measured under identical optical and electronic conditions avoiding the need for correction of the photomultiplier tube signal for the gating sequence. We demonstrate frequency domain background suppression using a mixture of short- and long-lifetime probes and for a long-lifetime probe in human plasma with significant autofluorescence.

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Metal complexes as components of luminescent signalling systems

Cited by 76 publications

References 34 publications

Lanthanide-Containing Molecular and Supramolecular Polymetallic Functional Assemblies

Lanthanide-Containing Molecular and Supramolecular Polymetallic Functional Assemblies

The Coumarin Moiety as Chromophore of Fluorescent Ion Indicators in Biological Systems

Real-time background suppression during frequency domain lifetime measurements

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