Metal coordination polymer induced perylene probe excimer fluorescence and its application in acetylcholinesterase sensing and alpha-fetoprotein immunoassay

Abstract

Perylene probe excimer fluorescence based acetylcholinesterase sensing and alpha-fetoprotein immunoassay.

Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish

“…To date, a number of colorimetric, electrochemical, and fluorescent assays for AChE detection have been developed. However, most of these assays were based on enzyme-involved cascade reactions in which AChE converts ACh into choline − or acetylthiocholine into thiocholine, − the formed choline or thiocholine reacted with additional substrates (such as conjugated polymers, silver nanoclusters, and quantum dots) and produce a detectable signal. The biggest drawback of these methods was that they were not suitable for detecting AChE in living biological systems, although they effectively functioned in vitro.…”

Rational Design of N-Doped Carbon Nanocage-Equipped Co-Nx Active Sites for Oxidase Mimicking and Sensing Applications

“…The limit of detection (LOD, 3σ) for AChE is 0.2 mU L –1 . Relative to other reported methods (Table S4) for detecting AChE activity, such as colorimetric − and fluorescent − assays, the method here promises a high sensitivity with a very low LOD.…”

Polypeptide induced perylene probe excimer formation and its application in the noncovalent ratiometric detection of matrix metalloproteinase activity

“…S2, ESI †). 30,32 The samples were dissolved in deuterated chloroform solvent. The UV-vis absorption and fluorescence emission spectra of 10 mM PC1 were measured, as shown in Fig.…”

“…Previously, we have reported the self-assembly of small molecule fluorescent probes for the detection of lysozyme, heparinase, acetylcholine esterase, alkaline phosphatase, DNA methyltransferase and other proteins. [29][30][31][32][33] In this work, a fluorescence approach based on a perylene derivative modified with carboxyl groups at both ends (named PC1, Fig. S1 and S2, ESI †) was developed.…”