Metal-Doped Boron Quantum Dots for Versatile Detection of Lactate and Fluorescence Bioimaging

Abstract

To improve the stability and fluorescence (FL) of monoelemental boron nanomaterials, this work put forward a metal-coordination strategy to explore emerging metal-doped boron quantum dots, Co@BQDs. Through theoretical calculations, B−Co bonding as predicted can suppress the B−O reaction and protect the electronic structures of exfoliated twodimensional (2D) boron from oxidation and decomposition upon exposure to oxygen. In experimental studies, Co 2+ was added into a dispersion liquid of bulk boron and subjected to probe sonication to promote Co 2+ adsorption on the surface of exfoliated 2D boron, followed by Co 2+ coordination with exposed boron atoms. Solvothermal treatment of exfoliated 2D boron resulted in the generation of Co 2+ -doped 0D boron Co@BQDs. Experimental results confirm that Co@BQDs have higher colloidal and FL stability than BQDs as a reference. B−Co bonding formation to suppress the B−O reaction ensures the high stability of exfoliated boron structures. A dispersion liquid of Co@BQDs with stable and bright FL was used for visual FL imaging of solutions and solid substrates. Based on enzymatic and cascade oxidation-induced FL quenching of Co@BQDs, a novel FL bio-probe of lactate was explored. This bio-probe, with a broad detection range of 0.01−10 mM and a low detection limit of 3.1 μM, enables FL sensing of lactate in biosamples and shows high detection recoveries of 98.0−102.8%. Moreover, this bio-probe realized versatile FL imaging and visual detection of lactate in liquid/solid-phase systems. These results demonstrate great prospects of Co@BQDs as emerging and efficient imaging reagents for long-term tracking and bioimaging applications.

Borophene Quantum Dots with Strong Photoluminescence for Selective Metal Ion Sensing

“…Yet, a more comprehensive exploration of the nonlinearity could offer valuable insights into diverse charge-transfer pathways and quenching mechanisms . The elucidation of exciton decay processes in various semiconductor materials often involves employing first-order rate equations . In this research, to comprehend the PL intensity quenching caused by Fe 3+ ions quantitatively, we employed the first-order rate equation to model charge-transfer dynamics through two concurrent pathways normald N normald t = G − [ Γ 1 + Γ 3 + Γ 2 false( δ false) ] N In this context, N denotes the population of excitons within BQDs at a specific time t .…”

“…47 The elucidation of exciton decay processes in various semiconductor materials often involves employing first-order rate equations. 22 In this research, to comprehend the PL intensity quenching caused by Fe 3+ ions quantitatively, we employed the first-order rate equation to model charge-transfer dynamics through two concurrent pathways…”

Strategic Assessment of Boron-Enriched Carbon Dots/Naproxen: Diagnostic, Toxicity, and In Vivo Therapeutic Evaluation

“…The preceding research provides clear evidence of the considerable potential of boron-doped carbon nanoparticles in cancer therapy. , Intending to be further applied to clinical applications, additional research is required to evaluate the potential toxicological effects, both in vitro and in vivo, that may arise from the use of these materials in biomedical applications. Moreover, verifying their colloidal stability within a range of pH values and biological salt concentrations is crucial.…”

“…The cell viability was evaluated with a CCK-8 assay (Beyotime, China). Briefly, HeLa cells were seeded on each well of a 96-well plate at a density of 8 × 10 3 cells per well and cultured in a humidified 5% CO 2 incubator at 37 °C for 24 h. Then B-CDs were introduced into cells with various test concentrations (7,15,31,62,125,250, 500, and 1000 mg/mL) in a culture medium, and cells cultured without nanomaterials were taken as the control. The cells were washed with PBS after 24 h of incubation, followed by addition of 100 mL of fresh culture medium and 10 μL of CCK-8 into each well and an additional In Vitro Bioimaging of B-CDs.…”

Based on Aptamer-carbon quantum dots and silver nanoparticles FRET sensor for sensitive detection of E. coli

“…The aim of this study was to develop a novel, simple, rapid, non-toxic method with maximum possible ultra-sensitive detection of E. coli. Most CQDs are used for bioimaging of bacterial cells [14,15] . There are few studies on the detection of speci c bacteria as targets by CQDs.…”