Metal-inducible activities of metallothionein promoters in fish cells and fry

Inoue, Koji; Akita, Noriko; Shiba, Tadayoshi; Satake, Mikio; Yamashita, Shinya

doi:10.1016/0006-291x(92)91740-h

Cited by 22 publications

(5 citation statements)

References 26 publications

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“…As in other vertebrates, resistance to heavy metal toxicity in fish is related to their ability to overexpress MT genes after exposure to metal ions [31][32][33][34][35]. The results of our previous studies showed an almost undetectable steady-state concentration of endogenous MT in icefish liver [25].…”

Section: Discussionmentioning

confidence: 87%

Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels

Carginale

Scudiero

Capasso

et al. 1998

Biochemical Journal

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Reverse transcriptase-mediated PCR has been used to isolate two distinct metallothionein (MT) cDNA species from RNA extracted from icefish liver, namely MT-I and MT-II. Northern blot analysis with these cDNA species revealed that significant endogenous levels of MT mRNA were present in liver tissues of normal animals despite the fact that no MT protein could be found accumulating in the same tissue. However, multiple injections of CdCl2 induced high levels of both MT mRNA and MT protein. Sequence analysis of the cDNA species that were present after cadmium injection revealed the presence of both isoforms. Quantification of the MT-I and MT-II transcripts from normal and heavy-metal-treated fish showed an alteration in the ratio of the MT isoform transcripts. Endogenous transcripts consisted mostly of MT-II, whereas the MT-I transcript was preferentially accumulated only in response to the cadmium salt. The protein encoded by each cDNA isoform was isolated from the heavy-metal-treated fish and the availability of the specific MT mRNA for translation was demonstrated by translation in vitro. These results show that: (1) there is a discrepancy between the significant endogenous levels of MT mRNA and the absence of MT protein; (2) the accumulation of MT in icefish liver can be triggered by heavy metals; (3) genes encoding distinct MT isoforms are differentially regulated by heavy metals.

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Section: Discussionmentioning

confidence: 87%

Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels

Carginale

Scudiero

Capasso

et al. 1998

Biochemical Journal

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“…[33]), metallothionein promoters (MT) [34], yeast Gal4 regulatory region [35], the T7 binary system [36], and heat-shock promoters [37]. Among all these, only MT [38,39] has been studied more extensively in fish.…”

Section: Discussionmentioning

confidence: 99%

Heat-Inducible Expression of a Reporter Gene Detected by Transient Assay in Zebrafish

Ádám

Bártfai

Lele

et al. 2000

Experimental Cell Research

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“…A mixture of 0.01 Ixg of receptor plasmid (pCMV-rtGR-I or II) and 0.2 ~tg of reporter plasmid (pMSG-CAT) were cotransfected into subconfluent cultures of RBCF-1 cells in each well of 6-well dishes (Falcon) using calcium phosphate coprecipitation [24,25]. After 4 h, the precipitate was removed, and the cultures incubated for 48 h in fresh medium containing various concentrations of steroids supplemented with 1% FCS.…”

Section: Transient Gr Expression In Cultured Cellsmentioning

confidence: 99%

Fish glucocorticoid receptor with splicing variants in the DNA binding domain

et al. 1996

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Here we describe the isolation of a rainbow trout cDNA containing an entire GR coding region. Although the encoded protein is highly homologous to other GRs, especially in its DNA binding domain, it contains a nine amino acid insertion between the two zinc fingers. This novel form is found in all rainbow trout tissues examined; however, the testis also contains a splice variant lacking this insert, making it completely continuous to other GRs. In transient transfection assays of cultured cells, the two rainbow trout GR variants activated transcription from the glucocorticoid-responsive mouse mammary tumor virus promoter to comparable levels.

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Metal-inducible activities of metallothionein promoters in fish cells and fry

Cited by 22 publications

References 26 publications

Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels

Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels

Heat-Inducible Expression of a Reporter Gene Detected by Transient Assay in Zebrafish

Fish glucocorticoid receptor with splicing variants in the DNA binding domain

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