Metal nanoparticles and nanoparticle composites are effective against Haemophilus influenzae, Streptococcus pneumoniae, and multidrug-resistant bacteria

Huang, Yushan; Wang, Jann-Tay; Tai, Hui-Ming; Chang, Pai‐Chun; Huang, H.

doi:10.1016/j.jmii.2022.05.003

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…These findings suggest that the combination of Se-NPs with antibiotics and antimicrobial peptides may represent a highly effective new strategy with broad antibacterial (32). The ability of Se-NPs to decrease antibiotic resistance in various bacteria has been demonstrated in previous studies (33)(34)(35)(36), highlighting their potential as a valuable tool in the fight against antibiotic-resistant infections.…”

Section: Discussionmentioning

confidence: 69%

Effect of Selenium Nanoparticles on the Expression of OqxBGene in Clinical Isolates of Klebsiella pneumoniae

Abdolmasoudi,

Kariminik,

Ferdousi

2023

Avicenna J Clin Microbiol Infect

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Background: OqxB is an efflux pump that has emerged as a factor contributing to antibiotic resistance in Klebsiella pneumoniae. The objective of this study was to investigate the occurrence of AcrAB efflux pump resistance genes in clinical samples of K. pneumoniae and to evaluate the influence of selenium nanoparticles (Se-NPs), loaded with ampicillin, on the expression of the OqxB gene associated with efflux pumps. Methods: A total of 500 clinical samples were collected from hospitalized patients, and 60 strains of K. pneumoniae were isolated using standard microbiological methods. These strains were then analyzed using phenotypic and polymerase chain reaction (PCR) techniques to detect the frequency of KPSM, MRK, OqxA, and OqxB genes through multiplex PCR. The impact of Se-NPs loaded with ampicillin on the expression of the OqxB resistance gene was investigated using a real-time PCR technique. Results: Based on the results of this study, it was found that the KPSM gene is not present in any of the investigated K. pneumoniae isolates. However, the MRK, OqxA, and OqxB genes were detected in 57, 55, and 54 isolates, respectively. The minimum inhibitory concentration (MIC) values for Se-NP and Se-NPs with ampicillin were reported to be 1500 μg/mL and 375 μg/mL, respectively. Notably, the SeNPs with ampicillin could significantly down-regulate the expression of the OqxB gene. These findings demonstrated the potential of Se-NPs as a promising strategy for reducing antibiotic resistance in K. pneumoniae infections. Conclusion: The findings emphasize the notable prevalence of drug resistance genes, specifically those associated with efflux pump production, in clinical samples of K. pneumoniae. Remarkably, the utilization of Se nanoparticles loaded with ampicillin demonstrated its efficacy in suppressing the expression of the OqxB gene and enhancing bacterial susceptibility to ampicillin. The results further imply that Se-NPs could serve as a promising avenue for the development of innovative antibacterial agents, aimed at combating antibiotic resistance in K. pneumoniae infections.

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Section: Discussionmentioning

confidence: 69%

Effect of Selenium Nanoparticles on the Expression of OqxBGene in Clinical Isolates of Klebsiella pneumoniae

Abdolmasoudi,

Kariminik,

Ferdousi

2023

Avicenna J Clin Microbiol Infect

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“…Ag NPs have proved to be effective, also, in treating H. influenzae and carbapenemresistant P. aeruginosa isolates [209].…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

New Antimicrobial Strategies to Treat Multi-Drug Resistant Infections Caused by Gram-Negatives in Cystic Fibrosis

Scoffone,

Barbieri,

Irudal

et al. 2024

Antibiotics

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People with cystic fibrosis (CF) suffer from recurrent bacterial infections which induce inflammation, lung tissue damage and failure of the respiratory system. Prolonged exposure to combinatorial antibiotic therapies triggers the appearance of multi-drug resistant (MDR) bacteria. The development of alternative antimicrobial strategies may provide a way to mitigate antimicrobial resistance. Here we discuss different alternative approaches to the use of classic antibiotics: anti-virulence and anti-biofilm compounds which exert a low selective pressure; phage therapies that represent an alternative strategy with a high therapeutic potential; new methods helping antibiotics activity such as adjuvants; and antimicrobial peptides and nanoparticle formulations. Their mechanisms and in vitro and in vivo efficacy are described, in order to figure out a complete landscape of new alternative approaches to fight MDR Gram-negative CF pathogens.

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“… 11 Some nanomaterials, such as silver nanoparticles (AgNPs), selenium nanoparticles (SeNPs), and metal NPs solutions (ND50, NK99, and TPNT1), can be prepared as environmental sanitizers or as preventive or therapeutic inhalants due to their directive antibacterial or antiviral effects in vitro. 12 Moreover, NPs can be utilized for the delivery of drugs, enhancing their efficacy and reducing adverse reactions. It was shown that ethyl cellulose nanoparticles (EC-NPs) for amphotericin delivery had good stability, high bioavailability, and low cytotoxicity, providing a potential delivery vehicle for oral drugs for the treatment of fungi and parasite infections.…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology’s frontier in combatting infectious and inflammatory diseases: prevention and treatment

Huang,

Guo,

et al. 2024

Sig Transduct Target Ther

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Inflammation-associated diseases encompass a range of infectious diseases and non-infectious inflammatory diseases, which continuously pose one of the most serious threats to human health, attributed to factors such as the emergence of new pathogens, increasing drug resistance, changes in living environments and lifestyles, and the aging population. Despite rapid advancements in mechanistic research and drug development for these diseases, current treatments often have limited efficacy and notable side effects, necessitating the development of more effective and targeted anti-inflammatory therapies. In recent years, the rapid development of nanotechnology has provided crucial technological support for the prevention, treatment, and detection of inflammation-associated diseases. Various types of nanoparticles (NPs) play significant roles, serving as vaccine vehicles to enhance immunogenicity and as drug carriers to improve targeting and bioavailability. NPs can also directly combat pathogens and inflammation. In addition, nanotechnology has facilitated the development of biosensors for pathogen detection and imaging techniques for inflammatory diseases. This review categorizes and characterizes different types of NPs, summarizes their applications in the prevention, treatment, and detection of infectious and inflammatory diseases. It also discusses the challenges associated with clinical translation in this field and explores the latest developments and prospects. In conclusion, nanotechnology opens up new possibilities for the comprehensive management of infectious and inflammatory diseases.

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Metal nanoparticles and nanoparticle composites are effective against Haemophilus influenzae, Streptococcus pneumoniae, and multidrug-resistant bacteria

Cited by 12 publications

References 35 publications

Effect of Selenium Nanoparticles on the Expression of OqxBGene in Clinical Isolates of Klebsiella pneumoniae

Effect of Selenium Nanoparticles on the Expression of OqxBGene in Clinical Isolates of Klebsiella pneumoniae

New Antimicrobial Strategies to Treat Multi-Drug Resistant Infections Caused by Gram-Negatives in Cystic Fibrosis

Nanotechnology’s frontier in combatting infectious and inflammatory diseases: prevention and treatment

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