Metal‐Phenolic Network Directed Coating of Single Probiotic Cell Followed by Photoinitiated Thiol‐Ene Click Fortification to Enhance Oral Therapy

Abstract

Probiotics‐based oral therapy has become a promising way to prevent and treat various diseases, while the application of probiotics is primarily restricted by loss of viability due to adverse conditions in the gastrointestinal (GI) tract during oral delivery. Layer‐by‐layer (LbL) single‐cell encapsulation approaches are widely employed to improve the bioavailability of probiotics. However, they are generally time‐ and labor‐intensive owing to multistep operation. Herein, a simple yet efficient LbL technique is developed to coat a model probiotic named Escherichia coli Nissle 1917 (EcN) through polyphenol‐Ca2+ network directed allyl‐modified gelatin (GelAGE) adsorption followed by cross‐linking of GelAGE via photoinitiated thiol‐ene click reaction to protect EcN from harsh microenvironments of GI tract. LbL single‐cell encapsulation can be performed within 1 h through simple operation. It is revealed that coated EcN exhibits significantly improved viability against acidic gastric fluid and bile salts, and enhanced colonization in the intestinal tract without loss of proliferation capabilities. Furthermore, oral therapy of coated EcN remarkably relieves the pathological symptoms associated with colitis in mice including down‐regulating inflammation, repairing epithelial barriers, scavenging reactive oxygen species (ROS), and restoring the homeostasis of gut microbiota. This simplified LbL coating strategy has great potential for various probiotics‐mediated biomedical and nutraceutical applications.

Polyphenolic Nanoparticle-Modified Probiotics for Microenvironment Remodeling and Targeted Therapy of Inflammatory Bowel Disease

“…Escherichia coli Nissle 1917 (EcN) is a commercially available probiotic, which can potentially impact the treatment of IBD through improving the bacterial diversity within the colon. , However, the successful delivery of probiotics faces challenges not only to the route of administration but also to the inherent pathological processes . Recently, a wide range of probiotics encapsulation strategies have been constructed and achieved success in improving the probiotics delivery. − However, the current encapsulation strategies focus on improving the resistance and colonization of probiotics, but few can address the oxidant and proinflammatory microenvironment, which is another key factor for intestinal homeostasis. Consequently, such a collaboratively therapeutic approach which facilitates the effective modulation of intestinal homeostasis by regulating the redox balance, mitigating inflammation, and remodeling gut microbiota is promising.…”