Metal resistance in Acidocella strains and plasmid-mediated transfer of this characteristic to Acidiphilium multivorum and Escherichia coli

Sixty six isolates of Pseudomonas spp. were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weight 27 Kb for nickel and lead. It was identified as Pseudomonas aeruginosa ASU 6a. Its minimal inhibitory concentration (MIC) for Cu(2+), Co(2+), Ni(2+), Zn(2+), Cr(3+), Cd(2+)and Pb(2+) were 6.3, 5.9, 6.8, 9.2, 5.8, 4.4, and 3.1 mM, respectively. Growth kinetics and the maximum adsorption capacities were determined under Ni(2+) and Pb(2+) stress. The latter heavy metals induced potassium efflux and were used as indicator for plasma membrane permeabilization.

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“…The growth rate and growth parameters of resistant strain P. aeruginosa ASU 6a to lead and nickel were determined according to [27,28].…”

Section: Effect Of Lead and Nickel On The Growth Kinetics Of P Aerugmentioning

confidence: 99%

Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city, Egypt

Hassan

Abskharon

El-Rab

et al. 2008

J. Basic Microbiol.

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“…strain GS19h was chosen. Since one or more plasmids of this strain harbour metal resistance conferring gene(s) [11], it is obvious that its plasmid-less derivatives will be more sensitive to metals. This report af-¢rms that curing of plasmids may be achieved arti¢cially by applying physical methods even in the case of acidophilic bacteria of mine environment.…”

Section: Discussionmentioning

confidence: 99%

Plasmid curing from an acidophilic bacterium of the genus Acidocella

Ghosh

2000

FEMS Microbiology Letters

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Preservation of the acidophilic heterotroph, Acidocella sp. strain GS19h, at 4°C in stab culture eliminated all indigenous plasmids from this bacterium. Growth at 42°C initially caused changes in the plasmid profile followed by total elimination of plasmids after 10 cycles of growth. Concomitant to this loss of all plasmids, the cured derivatives became sensitive to CdSO4 and ZnSO4, and the MIC value of the salts dropped from 1 M for each in the case of parental strain to 2 mM and 5 mM, respectively, suggesting plasmid‐mediated inheritance of metal resistance in this bacterium. The cured derivatives could not utilise lactose, indicating this metabolic activity to be plasmid‐associated in this strain.

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“…strain GS19h was chosen. Since one or more plasmids of this strain harbour metal resistance conferring gene(s) [11], it is obvious that its plasmid‐less derivatives will be more sensitive to metals. This report affirms that curing of plasmids may be achieved artificially by applying physical methods even in the case of acidophilic bacteria of mine environment.…”

Section: Discussionmentioning

confidence: 99%

“…Plasmid was prepared following an alkaline lysis method as described before [11]. Normal gel electrophoresis of plasmids was carried out with 0.7‐0.8% (w/v) agarose.…”

Section: Methodsmentioning

confidence: 99%

Plasmid curing from an acidophilic bacterium of the genusAcidocella

Ghosh

Mahapatra

Ramamurthy

et al. 2000

FEMS Microbiology Letters

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Preservation of the acidophilic heterotroph, Acidocella sp. strain GS19h, at 4 degrees C in stab culture eliminated all indigenous plasmids from this bacterium. Growth at 42 degrees C initially caused changes in the plasmid profile followed by total elimination of plasmids after 10 cycles of growth. Concomitant to this loss of all plasmids, the cured derivatives became sensitive to CdSO(4) and ZnSO(4), and the MIC value of the salts dropped from 1 M for each in the case of parental strain to 2 mM and 5 mM, respectively, suggesting plasmid-mediated inheritance of metal resistance in this bacterium. The cured derivatives could not utilise lactose, indicating this metabolic activity to be plasmid-associated in this strain.

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Metal resistance in Acidocella strains and plasmid-mediated transfer of this characteristic to Acidiphilium multivorum and Escherichia coli

Cited by 38 publications

References 31 publications

Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city, Egypt

Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city, Egypt

Plasmid curing from an acidophilic bacterium of the genus Acidocella

Plasmid curing from an acidophilic bacterium of the genusAcidocella

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