Metal uptake and distribution in the zebrafish (Danio rerio) embryo: differences between nanoparticles and metal ions

Böhme, Steffi; Baccaro, Marta; Schmidt, Matthias; Potthoff, Annegret; Stark, H.; Reemtsma, Thorsten; Kühnel, Dana

doi:10.1039/c6en00440g

Cited by 44 publications

(39 citation statements)

References 44 publications

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“…Since ROS were highly localized in zebrafish treated with 1 , we explored methods for determining drug localization in vivo . Laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS), 50 nanoscale secondary ion mass spectrometry (nanoSIMS) imaging, 51 and electron microscopy 52 have been explored for the detection of metal ions and nanoparticles in zebrafish. Confocal microscopy and fluorescent complex 6 provide an alternative strategy, which utilizes the transparent properties of zebrafish embryos.…”

Section: Resultsmentioning

confidence: 99%

In Vivo Selectivity and Localization of Reactive Oxygen Species (ROS) Induction by Osmium Anticancer Complexes That Circumvent Platinum Resistance

et al. 2018

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Platinum drugs are widely used for cancer treatment. Other precious metals are promising, but their clinical progress depends on achieving different mechanisms of action to overcome Pt-resistance. Here, we evaluate 13 organo-Os complexes: 16-electron sulfonyl-diamine catalysts [(η6-arene)Os(N,N′)], and 18-electron phenylazopyridine complexes [(η6-arene)Os(N,N’)Cl/I]+ (arene = p-cymene, biphenyl, or terphenyl). Their antiproliferative activity does not depend on p21 or p53 status, unlike cisplatin, and their selective potency toward cancer cells involves the generation of reactive oxygen species. Evidence of such a mechanism of action has been found both in vitro and in vivo. This work appears to provide the first study of osmium complexes in the zebrafish model, which has been shown to closely model toxicity in humans. A fluorescent osmium complex, derived from a lead compound, was employed to confirm internalization of the complex, visualize in vivo distribution, and confirm colocalization with reactive oxygen species generated in zebrafish.

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Section: Resultsmentioning

confidence: 99%

In Vivo Selectivity and Localization of Reactive Oxygen Species (ROS) Induction by Osmium Anticancer Complexes That Circumvent Platinum Resistance

et al. 2018

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“…In contrast, Muth-Kohne et al [28] reported a decrease in egg yolk sac size following exposure to AgNPs (< 20 nm size) at 48 hpf, using a significantly higher dose of 73 mg/L. It is known that silver can accumulate in tissues both in the form of ions and in the form of nanoparticles [29]. The silver content measurement result in the present study could not ascertain the form of metal inside the tissue, which is one of the drawbacks of using ICP-MS to examine the uptake of nanometals.…”

Section: Discussionmentioning

confidence: 96%

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Qiang

Arabeyyat

et al. 2020

IJMS

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Silver nanoparticles (AgNPs) are widely used in commercial applications as antimicrobial agents, but there have recently been increasing concerns raised about their possible environmental and health impacts. In this study, zebrafish embryos were exposed to two sizes of AgNP, 4 and 10 nm, through a continuous exposure from 4 to 96 h post-fertilisation (hpf), to study their uptake, impact and molecular defense responses. Results showed that zebrafish embryos were significantly impacted by 72 hpf when continuously exposed to 4 nm AgNPs. At concentrations above 0.963 mg/L, significant in vivo uptake and delayed yolk sac absorption was evident; at 1.925 mg/L, significantly reduced body length was recorded compared to control embryos. Additionally, 4 nm AgNP treatment at the same concentration resulted in significantly upregulated hypoxia inducible factor 4 (HIF4) and peroxisomal membrane protein 2 (Pxmp2) mRNA expression in exposed embryos 96 hpf. In contrast, no significant differences in terms of larvae body length, yolk sac absorption or gene expression levels were observed following exposure to 10 nm AgNPs. These results demonstrated that S4 AgNPs are available for uptake, inducing developmental (measured as body length and yolk sac area) and transcriptional (specifically HIF4 and Pxmp2) perturbations in developing embryos. This study suggests the importance of particle size as one possible factor in determining the developmental toxicity of AgNPs in fish embryos.

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“…[10][11][12] Only recently, some studies have appeared in which different forms of NMs were reported in tissues of organisms, providing insights into form-specific uptake of NMs. [13][14][15][16] Methods to quantify form-specific concentrations of NMs such as single particle inductively coupled plasma mass spectroscopy (sp-ICP-MS) for metal-based NMs are becoming more available. These developments in analytical methods have enabled modelling approaches that could reveal patterns of form-specific uptake and accumulation of NMs in organisms, such as aspects of absorption, distribution, metabolism and excretion (ADME).…”

Section: Introductionmentioning

confidence: 99%

Tools and rules for modelling uptake and bioaccumulation of nanomaterials in invertebrate organisms

Brink

Kokalj

Silva

et al. 2019

Environ. Sci.: Nano

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Metal uptake and distribution in the zebrafish (Danio rerio) embryo: differences between nanoparticles and metal ions

Abstract: Quantitative data on nanoparticle and cation uptake are compared in a compartment-specific way and distinct differences between metals were identified.

Cited by 44 publications

References 44 publications

In Vivo Selectivity and Localization of Reactive Oxygen Species (ROS) Induction by Osmium Anticancer Complexes That Circumvent Platinum Resistance

In Vivo Selectivity and Localization of Reactive Oxygen Species (ROS) Induction by Osmium Anticancer Complexes That Circumvent Platinum Resistance

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Tools and rules for modelling uptake and bioaccumulation of nanomaterials in invertebrate organisms

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