Metallated porphyrin-doped Conjugated Polymer Nanoparticles for Efficient Photodynamic Therapy of Brain and Colorectal Tumor Cells

Abstract

CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis.

Colorectal cancer combination therapy using drug and gene co-delivered, targeted poly(ethylene glycol)-ε-poly(caprolactone) nanocarriers

“…The cytotoxicity of Caco-2 and SW480 cells treated with NCs were evaluated by using MTT assay. 30 Cells were seeded into 96-well microplates at a concentration of 10 5 cells/mL and allowed to grow for 24 hours to a subconfluent state (80% confluence). The culture medium was replaced with fresh medium supplemented with 10% FBS having different concentrations of free 5-FU (5-FU) or different kinds of NCs, and samples were incubated for 72 hours with 100 µL of tested suspensions.…”

Understanding the glioblastoma tumor biology to optimize photodynamic therapy: From molecular to cellular events

“…In CPNs, the polymer matrix efficiently collects photons and transfers excitation energy to PS dopants within the particle. Furthermore, in the metallated porphyrin‐doped conjugated polymer nanoparticles used in that work, the excited dopants amplify the particle's ability to generate 1 O 2 (Ibarra et al., 2018). Also, the nanoparticle system allowed the implementation of a hydrophobic porphyrin never used in PDT, such as octaethylporphyrin, by providing colloidal stability to the molecular PS in physiological media.…”

“…Nowadays and with the growing development of nanotechnology, multifunctional nanoparticle systems are designed to converge several functions in a unique particle; for instance, the delivery of hydrophobic PSs (Ibarra et al., 2018), selective recognition of GBM cell membrane receptors (Rajora et al., 2017), improvement of PS antitumor efficacy (Ibarra et al., 2018; Miretti et al, 2020). Accordingly, our group is working in the development and preclinical evaluation of conjugated polymer nanoparticles (CPNs) assembled with dopant molecular PSs with the aim to increase 1 O 2 quantum yield.…”

Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors

“…Aimed to this, our group has recently developed metallated porphyrin-doped conjugated polymer nanoparticles (CPNs) for highly efficient photodynamic therapy (PDT), a therapeutic approach of growing interest in the treatment of GBM and the prevention of local tumor recurrence [ 7 ]. These CPNs have been shown to be effective at eliminating glioma tumor cells through ROS-induced apoptotic damage thus highlighting their potential use in photo-assisted treatment of GBM [ 8 , 9 , 10 ]. Owing to their excellent light-harvesting and photoemission properties combined with their amenable surface-functionalization to target specific cells, CPNs have been successfully applied as target-specific labels for both in vitro and in vivo fluorescence imaging and identification of cancer cells.…”

“…Owing to their excellent light-harvesting and photoemission properties combined with their amenable surface-functionalization to target specific cells, CPNs have been successfully applied as target-specific labels for both in vitro and in vivo fluorescence imaging and identification of cancer cells. Additionally, by incorporating carefully chosen molecular dopants into CPNs their capacity as photosensitizers for singlet oxygen ( 1 O 2 ) production can be greatly increased and applied for tumor reduction/elimination [ 8 , 11 ].…”