Metallic ions as therapeutic agents in tissue engineering scaffolds: an overview of their biological applications and strategies for new developments

Mouriño, Viviana; Cattalini, Juan Pablo; Boccaccını, Aldo R.

doi:10.1098/rsif.2011.0611

Cited by 388 publications

(281 citation statements)

References 237 publications

(272 reference statements)

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“…Even though it is now well accepted that ion dissolution products of bioactive silicate glasses are able to stimulate osteogenesis, as well as angiogenesis, 6 further detailed investigations are required to find out whether and how the ion release of 45S5 Bioglass Ò -based scaffolds or likewise the surface properties of the scaffold material affect the proliferation and tube formation of HUVECs, as well as the growth factor secretion of cells. Concededly, further in vitro and in vivo investigations are required to generate relevant information on the angiogenic potential of cell seeded 45S5 Bioglass Ò -based scaffolds and other bioactive glass compositions were specific (metal) ions with possible angiogenic effect (e.g., Cu) 6,45 are included in the silicate matrix.…”

Section: Resultsmentioning

confidence: 99%

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Handel

Hammer

Nooeaid

et al. 2013

Tissue Engineering Part A

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Poor vascularization is the key limitation for long-term acceptance of large three-dimensional (3D) tissue engineering constructs in regenerative medicine. 45S5 Bioglass Ò was investigated given its potential for applications in bone engineering. Since native Bioglass Ò shows insufficient angiogenic properties, we used a collagen coating, to seed human adipose tissue-derived stem cells (hASC) confluently onto 3D 45S5 Bioglass Ò -based scaffolds. To investigate vascularization by semiquantitative analyses, these biofunctionalized scaffolds were then subjected to in vitro human umbilical vein endothelial cells formation assays, and were also investigated in the chorioallantoic membrane (CAM) angiogenesis model, an in vivo angiogenesis assay, which uses the CAM of the hen's egg. In their native, nonbiofunctionalized state, neither Bioglass Ò -based nor biologically inert fibrous polypropylene control scaffolds showed angiogenic properties. However, significant vascularization was induced by hASC-seeded scaffolds (Bioglass Ò and polypropylene) in the CAM angiogenesis assay. Biofunctionalized scaffolds also showed enhanced tube lengths, compared to unmodified scaffolds or constructs seeded with fibroblasts. In case of biologically inert hernia meshes, the quantification of vascular endothelial growth factor secretion as the key angiogenic stimulus strongly correlated to the tube lengths and vessel numbers in all models. This correlation proved the CAM angiogenesis assay to be a suitable semiquantitative tool to characterize angiogenic effects of larger 3D implants. In addition, our results suggest that combinations of suitable scaffold materials, such as 45S5 Bioglass Ò , with hASC could be a promising approach for future tissue engineering applications.

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Section: Resultsmentioning

confidence: 99%

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Handel

Hammer

Nooeaid

et al. 2013

Tissue Engineering Part A

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“…In recent years, more and more evidences show that bioactive ions play important roles in regulating cell behaviors including cell differentiation 11. Some studies have shown that zinc promoted adipogenesis of rat adipocytes and stimulated the conversion of glucose to lipids in 3T3‐L1 fibroblasts and adipocytes in vitro and in vivo,12 suggesting that some bioactive ions indeed can function as stimuli in promoting adipogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Silicon‐Enhanced Adipogenesis and Angiogenesis for Vascularized Adipose Tissue Engineering

et al. 2018

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The enhancement of adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and sufficient vascularization remain great challenges for the successful reconstruction of engineered adipose tissue. Here, the bioactive effects of silicon (Si) ions on adipogenic differentiation of human BMSCs (HBMSCs) and the stimulation of vascularization during adipose tissue regeneration are reported. The results show that Si ions can enhance adipogenic differentiation of HBMSCs through the stimulation of the expression of adipogenic differentiation switches such as peroxisome proliferator‐activated receptor γ and CCAAT/enhancer‐binding protein α. Furthermore, Si ions can enhance both angiogenesis and adipogenesis, and inhibit dedifferentiation of cocultured adipocytes by regulating the interactions between HBMSC‐derived adipocytes and human umbilical vein endothelial cells, in which the promotion of the expression of insulin‐like growth factor 1 and vascular endothelial growth factor plays vital roles. The in vivo studies further demonstrate that the designed composite hydrogel with the ability to release bioactive Si ions clearly stimulates neovascularization and adipose tissue regeneration. The study suggests that Si ions released from biomaterials are important chemical cues for adipogenic differentiation and biomaterials with the ability to release Si ions can be designed for adipose tissue engineering.

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“…For this reason, there is a general trend to upgrade properties with some of the well known therapeutical ions because of their biological action [14][15][16]. The development of the three families of glasses was together with an increase of the textural properties (surface area and pore volume) in going from the dense MPGs to the MBGs exhibiting over 500 m 2 /g of surface area and more than 0.5 cm 3 /g of pore volume.…”

Section: Introductionmentioning

confidence: 99%

Glasses in bone regeneration: A multiscale issue

Salinas

Vallet‐Regí

2016

Journal of Non-Crystalline Solids

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Highligths MBGs containing small amounts of Ga, Ce or Zn exhibit high in vitro bioactivity  The bioactive response is preserved after obtaining 3D scaffolds  These scaffolds are optimum candidates for bone tissue engineering applications  MBG scaffold with 4% ZnO exhibit bactericide action against S. Aureus  The effects of the extra ions is sometimes complex and needs to be investigated 2 Abstract 3D scaffolds based in mesoporous bioactive glasses (MBGs) are being widely investigated to use in bone tissue engineering (TE) applications. These scaffolds are often obtained by rapid prototyping (RP) and exhibit an array of interconnected pores in a hierarchy of sizes. The ordered mesopores network (around 4 nm in diameter), is optimal for the adsorption and release of bone inductor biomolecules, and the arrangement of macropores over 100 m facilitates the bone cell ingrowths and angiogenesis. Nevertheless MBGs composition can be varied almost infinitely at the atomic scale by including in the glass network oxides of inorganic elements with a therapeutic action. In this article the synthesis and characterization of MBG scaffolds based on the 80%SiO2-15%CaO-5%P2O5 (in mol-%) glass with substitutions up to 3.5% of Ga2O3 or Ce2O3 or 7.0% of ZnO is revisited. The substituent inclusion and the RP processing slightly decrease the surface area, the pore volume and the mesoporous order as well as their bioactive response in solutions mimicking blood plasma. However, these values still remain useful for bone TE applications. Results exhibiting the bactericide action of MBG scaffolds containing ZnO are also presented. KeywordsMesoporous bioactive glass scaffolds; Ga, Ce and Zn as therapeutical ions; bactericide capability; bone tissue engineering 3

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Metallic ions as therapeutic agents in tissue engineering scaffolds: an overview of their biological applications and strategies for new developments

Cited by 388 publications

References 237 publications

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Silicon‐Enhanced Adipogenesis and Angiogenesis for Vascularized Adipose Tissue Engineering

Glasses in bone regeneration: A multiscale issue

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Metallic ions as therapeutic agents in tissue engineering scaffolds: an overview of their biological applications and strategies for new developments

Cited by 388 publications

References 237 publications

45S5-Bioglass®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

45S5-Bioglass®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Silicon‐Enhanced Adipogenesis and Angiogenesis for Vascularized Adipose Tissue Engineering

Glasses in bone regeneration: A multiscale issue

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Product

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45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay