Metallic surface dynamics of genomic DNA and its nitrogenous bases: SERS assessment and theoretical considerations

Label-free human-disease characterization through circulating cell free DNA analysis using Raman Spectroscopy

“…However, interestingly a few differences were identi ed between genders, shown in all studied samples: female samples showed a unique peak at 914 cm -1 [35,43] while male samples had a unique peak at 963 cm -1 [35].…”

“…Analysis found 13 major peaks, all present both in ccfDNA and respective gDNA that are listed in Table 1 with their corresponding assignments. These peaks are present at 526-531 cm -1 [35][36], 762-767 cm -1 [37], 781-788 cm -1 [37], 885-887 cm -1 [37], 913-921cm -1 [35,43], 1005 cm -1 [38-39], 1063-1065 cm -1 [41], 1223-1246 cm -1 [44], 1460-1462 cm -1 [38], 1552-1553 cm -1 [42], 1650 cm -1 [44], 2889-2891 cm -1 [42], 2942-2944 cm -1 [42]. From those, 10 are also found in the MCF12A normal mammary cell line, while 9 are common between healthy female samples.…”

“…The 13 identi ed major peaks and their corresponding assignments are presented in Table 1. All of the identi ed peaks at 522 cm -1 [35][36], 740 cm -1 [37], 921cm -1 [35,43], 1003 cm -1 [38-39], 1101 cm -1 [50], 1223 cm -1 [51], 1372 cm -1 [51], 1398 cm -1 [52], 1456-1464 cm -1[38], 1545 cm -1 [39], 1603 cm -1 [52], 2889-2891 cm -1 [42] and 2942-2944 cm -1 [42], were found to be present in all patients. No differences were observed between samples from the same patient.…”

Label-Free Human Disease Characterization through Circulating Cell-Free DNA Analysis Using Raman Spectroscopy

“…Healthy male and female ccfDNA spectra both showed nine common major peaks at 525-529 cm −1 [36,37], 758-762 cm −1 [38], 884 cm −1 [38], 1002-1004 cm −1 [39,40], 1046-1052 cm −1 [41], 1345-1348 cm −1 [42], 1460 cm −1 [39], 2889-2891 cm −1 [43], and 2942-2944 cm −1 [43]. However, interestingly, a few differences were identified between genders, shown in all studied samples: female samples showed a unique peak at 914 cm −1 [36,44], while male samples had a unique peak at 963 cm −1 [36].…”

“…Healthy male and female ccfDNA spectra both showed nine common major pea 529 cm −1 [36,37], 758-762 cm −1 [38], 884 cm −1 [38], 1002-1004 cm −1 [39,40], 1046- [41], 1345-1348 cm −1 [42], 1460 cm −1 [39], 2889-2891 cm −1 [43], and 2942-2944 However, interestingly, a few differences were identified between genders, sh studied samples: female samples showed a unique peak at 914 cm −1 [36,44], w samples had a unique peak at 963 cm −1 [36]. Overall, we describe for the first time the biomolecular profile of ccfDNA through Raman spectroscopy, producing distinct spectra patterns of sam healthy subjects.…”