Metalloproteinases at the surface of small extrcellular vesicles in advanced ovarian cancer: Relationships with ascites volume and peritoneal canceromatosis index

Yunusova, N. V.; Patysheva, Marina; Molchanov, Sergey V.; Замбалова, Е. А.; Grigor’eva, Alina; Коломиец, Л. А.; Очиров, М. О.; Тамкович, С. Н.; Кондакова, И. В.

doi:10.1016/j.cca.2019.03.1621

Cited by 16 publications

(18 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MMP9+/MMP2+/ EMMPRIN+ subpopulation of CD9-positive blood plasma exosomes reduced the cancer risk in CPPs. Similar data were obtained for ovarian cancer (Yunusova et al, 2019). Subpopulation of MMP9+/MMP2+/EMMPRIN+ at the surface of blood plasma exosomes in high-volume ascites ovarian cancer patients was reduced compared to that in low-volume ascites patients.…”

Section: Discussionsupporting

confidence: 81%

“…The morphology of blood plasma exosomes isolated from CRCPs was found to have no difference with that of patients with other cancers (Yunusova et al, 2019;Tugutova et al, 2019;Tamkovich et al, 2019). Moreover, we showed that the levels of 20S proteasomes in exosomes, MMP9+ and MMP9+/MMP2+/EMMPRIN+ exosome subpopulations in CD9-positive exosomes were significant for predicting colorectal cancer risk in CPPs.…”

Section: Discussionmentioning

confidence: 71%

“…Blood plasma exosomes were isolated using ultrafiltration with ultracentrifugation (Yunusova et al, 2019). Exosome samples were aliquoted and stored either at −80°C or in liquid nitrogen.…”

Section: Exosome Isolationmentioning

confidence: 99%

See 2 more Smart Citations

Exosomal Protease Cargo as Prognostic Biomarker in Colorectal Cancer

Yunusova

Замбалова

Patysheva

et al. 2021

Asian Pac J Cancer Prev

Self Cite

View full text Add to dashboard Cite

Objective: The aim of the study was to develop a model for predicting cancer risk in colorectal polyps' patients (CPPs), as well as to reveal additional prognosis factors for Stage III colorectal cancer based on differences in subpopulations of tetraspanins, tetraspanin-associated and tetraspanin-non-associated proteases in blood plasma exosomes of CPPs and colorectal cancer patients (CRCPs). Methods: The subpopulations of CD151-and Tspan8-positive exosomes, the subpopulations of metalloproteinase at the surface of СD9-positive exosomes and the level of 20S proteasomes in plasma exosomes in 15 CPPs (tubulovillous adenomas) and 60 CRCPs were evaluated using flow cytometry and Western blotting. Logistic regression analysis was performed to predict cancer risk of CPPs. Results: The levels of 20S proteasomes in exosomes, MMP9+, MMP9+/MMP2+/EMMPRIN+ in CD9-positive blood plasma exosomes are associated with the risk of malignant transformation of colorectal tubulovillous adenomas. In patients with Stage III CRC, the levels of 20S proteasomes (less than 2 units) and MMP9+ subpopulations (more than 61%) in plasma exosomes are unfavorable prognostic factors for overall survival. The levels of 20S proteasomes and ADAM10+/ ADAM17-subpopulations in CD9-positive blood plasma exosomes are the most significant values for predicting relapse-free survival. Conclusion: Protease cargo in CD9-positive blood plasma exosomes is prognostic biomarker for colorectal polyps and colorectal cancer.

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 71%

See 1 more Smart Citation

Exosomal Protease Cargo as Prognostic Biomarker in Colorectal Cancer

Yunusova

Замбалова

Patysheva

et al. 2021

Asian Pac J Cancer Prev

Self Cite

View full text Add to dashboard Cite

show abstract

“…The immunocytochemical identification of exosomes from plasma and total blood with monoclonal antibodies to tetraspanin CD9 was performed as described previously [ 45 ]. Quantitative analysis of the exosomal tetraspanines on the surface of the isolated EVs was carried out using flow cytometry, as described previously [ 46 ]. Flow cytometry was performed on the Cytoflex (Becman Coulter, USA), using the CytExpert 2.0 Software.…”

Section: Methodsmentioning

confidence: 99%

Total Blood Exosomes in Breast Cancer: Potential Role in Crucial Steps of Tumorigenesis

Konoshenko

Sagaradze

Orlova

et al. 2020

IJMS

View full text Add to dashboard Cite

Exosomes are crucial players in cell-to-cell communication and are involved in tumorigenesis. There are two fractions of blood circulating exosomes: free and cell-surface-associated. Here, we compared the effect of total blood exosomes (contain plasma exosomes and blood cell-surface-associated exosomes) and plasma exosomes from breast cancer patients (BCPs, n = 43) and healthy females (HFs, n = 35) on crucial steps of tumor progression. Exosomes were isolated by ultrafiltration, followed by ultracentrifugation, and characterized by cryo-electron microscopy (cryo-EM), nanoparticle tracking analysis, and flow cytometry. Cryo-EM revealed a wider spectrum of exosome morphology with lipid bilayers and vesicular internal structures in the HF total blood in comparison with plasma. No differences in the morphology of both exosomes fractions were detected in BCP blood. The plasma exosomes and total blood exosomes of BCPs had different expression levels of tumor-associated miR-92a and miR-25-3p, induced angiogenesis and epithelial-to-mesenchymal transition (EMT), and increased the number of migrating pseudo-normal breast cells and the total migration path length of cancer cells. The multidirectional effects of HF total blood exosomes on tumor dissemination were revealed; they suppress the angiogenesis and total migration path length of MCF10A, but stimulate EMT and increase the number of migrating MCF10A and the total path length of SKBR3 cells. In addition, HF plasma exosomes enhance the metastasis-promoting properties of SKBR3 cells and stimulate angiogenesis. Both cell-free and blood cell-surface-associated exosomes are involved in the crucial stages of carcinogenesis: the initiation of EMT and the stimulation of proliferation, cell migration, and angiogenesis. Thus, for the estimation of the diagnostic/prognostic significance of circulating exosomes in the blood of cancer patients more correctly, the total blood exosomes, which consist of plasma exosomes and blood cell-surface-associated exosomes should be used.

show abstract

“…For exosome immunoprecipitation and their subsequent analysis by fluorescence-activated cell sorting (FACS), 4 µm diameter aldehyde/sulfate latex beads (Interfacial Dynamics, Portland, Oregon, USA) were incubated with purified anti-CD9 or anti-CD24 (BD Biosciences, San Jose, CA, USA) or anti-ADAM-10 (Abcam, Cambridge, UK) antibodies at 22 • C overnight, with gentle agitation as previously described [13]. For FACS analysis, 30 µg exosomes were incubated with 3 × 10 5 anti-CD9, anti-CD24 or anti-ADAM-10 beads in 150 µL of PBS at 4 • C overnight, with gentle agitation.…”

Section: Flow Cytometry Analysismentioning

confidence: 99%

Proteomic Analysis of Blood Exosomes from Healthy Females and Breast Cancer Patients Reveals an Association between Different Exosomal Bioactivity on Non-tumorigenic Epithelial Cell and Breast Cancer Cell Migration in Vitro

et al. 2020

View full text Add to dashboard Cite

Exosomes are important intercellular communication vehicles, secreted into body fluids by multiple cell types, including tumor cells. They contribute to the metastatic progression of tumor cells through paracrine signalling. It has been recently discovered that blood circulating exosomes contain distinguishable fractions of free and cell-surface-associated vesicles. We evaluated the influence of protein cargoes from exosomes from plasma, and exosomes from the total blood of healthy females (HFs) and breast cancer patients (BCPs), on cell motility. We conducted a mass spectrometric analysis of exosomal contents isolated from samples using ultrafiltration and ultracentrifugation approaches and verified their nature using transmission electron microscopy, nanoparticle tracking analysis and flow cytometry. We observed that malignant neoplasm-associated proteins in exosomes from BCP total blood were detected more often than in plasma (66% vs. 59%). FunRich analysis to assess Gene Ontology (GO) enrichment revealed that proteins with catalytic activities, transporter functions and protein metabolism activities were increased in exosomes from BCP blood. Finally, GO analysis revealed that proteomic profiles of exosomes from HF total blood were enriched with proteins inhibiting cell migration and invasion, which explains the low stimulating activity of exosomes from HF total blood on SKBR-3 cancer cell migration velocity. This allows exosomes to act as intermediaries providing intercellular communications through horizontal transfer of RNA and functionally active proteins, potentially affecting the development of both primary neoplasms and distant metastases.

show abstract

Metalloproteinases at the surface of small extrcellular vesicles in advanced ovarian cancer: Relationships with ascites volume and peritoneal canceromatosis index

Cited by 16 publications

References 28 publications

Exosomal Protease Cargo as Prognostic Biomarker in Colorectal Cancer

Exosomal Protease Cargo as Prognostic Biomarker in Colorectal Cancer

Total Blood Exosomes in Breast Cancer: Potential Role in Crucial Steps of Tumorigenesis

Proteomic Analysis of Blood Exosomes from Healthy Females and Breast Cancer Patients Reveals an Association between Different Exosomal Bioactivity on Non-tumorigenic Epithelial Cell and Breast Cancer Cell Migration in Vitro

Contact Info

Product

Resources

About