Metalloproteinases facilitate connection of wound bed vessels to pre-existing skin graft vasculature

Knapik, Alicia; Hegland, Niels; Calcagni, Maurizio; Althaus, Martina; Vollmar, Brigitte; Giovanoli, Pietro; Lindenblatt, Nicole

doi:10.1016/j.mvr.2012.04.001

Cited by 9 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…33,34 In this study STSG vasculature degeneration as well as vessel ingrowth from the mouse wound bed was similar for freshly harvested and 7-day stored skin grafts. 33,34 In this study STSG vasculature degeneration as well as vessel ingrowth from the mouse wound bed was similar for freshly harvested and 7-day stored skin grafts.…”

Section: Discussionmentioning

confidence: 63%

In Vivo Evaluation of Wound Bed Reaction and Graft Performance After Cold Skin Graft Storage

Knapik¹,

Kornmann²,

Kerl³

et al. 2014

Journal of Burn Care & Research

Self Cite

View full text Add to dashboard Cite

Surplus harvested skin grafts are routinely stored at 4 to 6°C in saline for several days in plastic surgery. The purpose of this study was to evaluate the influence of storage on human skin graft performance in an in vivo intravital microscopic setting after transplantation. Freshly harvested human full-thickness skin grafts and split-thickness skin grafts (STSGs) after storage of 0, 3, or 7 days in moist saline at 4 to 6°C were transplanted into the modified dorsal skinfold chamber, and intravital microscopy was performed to evaluate vessel morphology and angiogenic change of the wound bed. The chamber tissue was harvested 10 days after transplantation for evaluation of tissue integrity and inflammation (hematoxylin and eosin) as well as for immunohistochemistry (human CD31, murine CD31, Ki67, Tdt-mediated dUTP-biotin nick-end labelling). Intravital microscopy results showed no differences in the host angiogenic response between fresh and preserved grafts. However, STSGs and full-thickness skin grafts exhibited a trend toward different timing and strength in capillary widening and capillary bud formation. Preservation had no influence on graft quality before transplantation, but fresh STSGs showed better quality 10 days after transplantation than 7-day preserved grafts. Proliferation and apoptosis as well as host capillary in-growth and graft capillary degeneration were equal in all groups. These results indicate that cells may activate protective mechanisms under cold conditions, allowing them to maintain function and morphology. However, rewarming may disclose underlying tissue damage. These findings could be translated to a new approach for the design of full-thickness skin substitutes.

show abstract

Section: Discussionmentioning

confidence: 63%

In Vivo Evaluation of Wound Bed Reaction and Graft Performance After Cold Skin Graft Storage

Knapik¹,

Kornmann²,

Kerl³

et al. 2014

Journal of Burn Care & Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…grafts exhibited the formation of large buds without transformation into capillary sprouts [33]. Furthermore, we found that matrix metalloproteinases (MMP) are intimately involved in capillary ingrowth and regression during skin graft revascularization ( Fig.…”

Section: Revascularization Of Skin Graftsmentioning

confidence: 89%

“…In fact, sprouting angiogenesis is an invasive process with: (i) proteolytic degradation of the endothelial basement membrane, (ii) cell migration with removal of matrix proteins, and (iii) matrix rearrangement to allow endothelial cells to form a proper lumen [34]. Accordingly, membrane type 1 (MT1)-MMP was detected at the sprout tips of microvessels growing into skin grafts [33]. In summary, skin graft revascularization is a dynamic combination of internal inosculation, angiogenesis, and vasculogenesis.…”

Section: Revascularization Of Skin Graftsmentioning

confidence: 99%

The Crucial Role of Vascularization and Lymphangiogenesis in Skin Reconstruction

et al. 2018

Self Cite

View full text Add to dashboard Cite

Background: The treatment of extensive skin defects and bradytrophic wounds remains a challenge in clinical practice. Despite emerging tissue engineering approaches, skin grafts and dermal substitutes are still the routine procedure for the majority of skin defects. Here, we review the role of vascularization and lymphangiogenesis for skin grafting and dermal substitutes from the clinician’s perspective. Summary: Graft revascularization is a dynamic combination of inosculation, angiogenesis, and vasculogenesis. The majority of a graft’s microvasculature regresses and is replaced by ingrowing microvessels from the wound bed, finally resulting in a chimeric microvascular network. After inosculation within 48–72 h, the graft is re-oxygenated. In contrast to skin grafts, the vascularization of dermal substitutes is slow and dependent on the ingrowth of vessel-forming angiogenic cells. Preclinical angiogenic strategies with adipose tissue-derived isolates are appealing for the treatment of difficult wounds and may markedly accelerate skin reconstruction in the future. However, their translation from bench to bedside is still restricted by major regulatory restrictions. Finally, the lymphatic system contributes to edema reduction and the removal of local wound debris. Therapeutic lymphangiogenesis is an emerging field of research in skin reconstruction. Key Messages: For the successful engraftment of skin grafts and dermal substitutes, the rapid formation of a microvascular network is of pivotal importance. Hence, to understand the biological processes behind revascularization of skin substitutes and to implement this knowledge into clinical practice is a prerequisite when treating skin defects. Furthermore, a functional lymphatic drainage crucially contributes to the engraftment of skin substitutes.

show abstract

“…In this procedure, lost skin is covered with healthy tissue or artificial composites (144,145) that provide the necessary elements (cells, growth factors, MMPs and scaffolds) for the healing process (146). The success of a skin transplant is primarily dependent on angiogenesis between the skin graft and the injury, which is predominantly mediated by MMP-2, 9 and 14 (147). Thus, SPs have been proposed as potential adjuvants to increase tissue compatibility during skin transplants.…”

Section: Potential Of Sps As Novel Alternatives For Wound Healing Carementioning

confidence: 99%

Practical context of enzymatic treatment for wound healing: A secreted protease approach (Review)

et al. 2020

View full text Add to dashboard Cite

Skin wounds have been extensively studied as their healing represents a critical step towards achieving homeostasis following a traumatic event. Dependent on the severity of the damage, wounds are categorized as either acute or chronic. To date, chronic wounds have the highest economic impact as long term increases wound care costs. Chronic wounds affect 6.5 million patients in the United States with an annual estimated expense of $25 billion for the health care system. Among wound treatment categories, active wound care represents the fastest-growing category due to its specific actions and lower costs. Within this category, proteases from various sources have been used as successful agents in debridement wound care. The wound healing process is predominantly mediated by matrix metalloproteinases (MMPs) that, when dysregulated, result in defective wound healing. Therapeutic activity has been described for animal secretions including fish epithelial mucus, maggot secretory products and snake venom, which contain secreted proteases (SPs). No further alternatives for use, sources or types of proteases used for wound healing have been found in the literature to date. Through the present review, the context of enzymatic wound care alternatives will be discussed. In addition, substrate homology of SPs and human MMPs will be compared and contrasted. The purpose of these discussions is to identify and propose the stages of wound healing in which SPs may be used as therapeutic agents to improve the wound healing process.

show abstract

Metalloproteinases facilitate connection of wound bed vessels to pre-existing skin graft vasculature

Cited by 9 publications

References 32 publications

In Vivo Evaluation of Wound Bed Reaction and Graft Performance After Cold Skin Graft Storage

In Vivo Evaluation of Wound Bed Reaction and Graft Performance After Cold Skin Graft Storage

The Crucial Role of Vascularization and Lymphangiogenesis in Skin Reconstruction

Practical context of enzymatic treatment for wound healing: A secreted protease approach (Review)

Contact Info

Product

Resources

About