Metallothionein and glutathione inLymnaea stagnalisdetermine the specificity of responses to the effects of ionising radiation

Abstract

The aim of our study was to distinguish the stress-related molecular response of the pulmonate mollusc Lymnaea stagnalis from the Chernobyl area in comparison with the consequences of other harmful effects, including the short-term effects of radiation and heating. Specimens inhabiting ponds near the Chernobyl nuclear power plant, the cooling channel of the electric power station and the soilreclamation channel (groups R, T and C, correspondingly), and specimens adapted to laboratory conditions (a control group (CL), a disposable group exposed to 2 mGy X-ray radiation over the body (RL), and a group exposed to 25°C for 4 days (TL)) were compared. Despite high variability of responses, Principle Component Analysis distinctly separated the laboratory and feral groups into two sets. In the feral groups, low levels of the stress-related and metal-binding protein metallothionein (MT), protein carbonyls and lactate dehydrogenase in the digestive gland were indicated. The main separating criteria selected by classification and regression tree analysis were the protein carbonyls, cholinesterase and MT. Molluscs from group R were clearly distinguished by the lowest levels of MT, Mn-superoxide dismutase and lactate dehydrogenase, and the highest level of glutathione, demonstrating that the oppression of the gene-determined stress-related response and its partially metabolic compensation can be possible markers for chronic environmental effects of irradiation.

Assessment of metal exposure (uranium and copper) by the response of a set of integrated biomarkers in a stream shredder

“…insects, mussels and fish) and stressors (e.g. organic load and temperature) (Gnatyshyna et al, 2012;Lavarías et al, 2017;Madeira et al, 2016). However, some researchers have proposed a bell-shaped dose-response relationship, in which an increasing stress first induces an increase in activity (overcompensating for ROS production), but at higher stressor concentrations, the enzyme can be depleted due to severe damage (Amiard et al, 2006;Chen et al, 2016aChen et al, , 2016bDagnino et al, 2007;Oliveira et al, 2012;Sadauskas-Henrique et al, 2017).…”

Evaluation of metallothionein response in Lymnaea stagnalis pond snail exposured to trace metals and thiocarbamate fungicide

“…From our previous experience, it is known that low weight cellular MT thiols belong to most sensitive biochemical indicators in L. stagnalis demonstrating a multiple variability of the metal composition and chromatographic forms under the environmental detrimental effects, and also, under the such nonspecific for this protein exposure as the effect of ionizing radiation [13,14]. In the current study, to attest their participation in metal detoxification and stress response, we evaluated total MT protein concentration (MT-SH), the level and composition of their metal-contained form (MT-Me) and their chromatographic characteristics.…”

Multi-marker Study of Dreissena polymorpha Populations from Hydropower Plant Reservoir and Natural Lake in Latvia

“…GSH provides the first line of defence as a ROS scavenger (Circu & Aw, 2008). The GSH level differences were the main distinguishing criteria for gastropods from a lake near the place of Chernobyl nuclear disaster (Gnatyshyna et al, 2012) and zebra mussels, transplanted to different sites for 14 -120 days (Falfushynska, Gnatyshyna, Stoliar, Dedourge-Geffard, & Geffard, 2010).…”