Metallothionein and Zinc Metabolism in Endotoxin Shock Rats

Abe, Shinya; Matsumi, Masaki; Tsukioki, Mitsunori; Mizukawa, S.; Takahashi, Takefumi; Iijima, Yoshio; Itano, Yoshitaro; Kosaka, Futami

doi:10.1007/978-3-0348-6784-9_61

Cited by 12 publications

(8 citation statements)

References 10 publications

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“…Wound inflammation induces cytokine release that may be involved in hepatic MT synthesis. Abe et al (1987) demonstrated the biological significance of the occurrence of hypozincemia following acute inflammation. They reported that hypozincemia benefits the infected host by enhancing migration and activity in macrophages and neutrophils.…”

Section: Discussionmentioning

confidence: 97%

“…A variety of physiological stress conditions, such as administration of endotoxin (Abe et al 1987), partial hepatectomy (Cain and Griffiths 1984;Ohtake et al 1978), and exposure to low temperature and strenuous exercise (Oh et al 1978), can lead to acute hypozincemia and he-286 Fig. 3A-H Time course of expression and localization of MT mRNA in the wounded skin.…”

Section: Discussionmentioning

confidence: 99%

“…Previous reports have taken notice of the biological functions of MT, especially those related to the metabolism of zinc as an essential trace element (Hamer 1986;Richards and Cousins 1975), as well as its functions as an acute phase protein providing protection against various stresses caused by inflammation and injury (Abe et al 1987;Hamer 1986;Itano et al 1991;Sobocinski et al 1978), proliferation, and regeneration of tissue (Brady and Webb 1981;Cain and Griffiths 1984;Wong and Klaassen 1979). Therefore, we considered it was important to investigate the possible roles of MT concerned with zinc metabolism and its influence on tissue damage and repair during wound healing.…”

Section: Introductionmentioning

confidence: 98%

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Zinc accumulation and metallothionein gene expression in the proliferating epidermis during wound healing in mouse skin

Iwata

Takebayashi

Ohta

et al. 1999

Histochemistry and Cell Biology

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Metallothionein (MT), a low molecular weight metal-binding protein, has been related to zinc and copper metabolism, the acute-phase response, and cellular proliferation. In this study, we investigated changes in zinc metabolism and MT gene expression occurring in tissue damage and repair during wound healing in mouse skin. Northern blot analysis revealed that a significant increase of MT mRNA was observed in the liver for 18 h after wounding, and serum zinc downfall and hepatic zinc uptake were observed. In situ hybridization analysis showed that no significant expression of MT mRNA was detected within the first 9 h after wounding. However, it was expressed restrictively in the proliferating epidermis of the wound margin after 12 h. Zinc began to accumulate in wounded skin after MT gene expressed. Northern blotting and immunocytochemical staining revealed that MT has been synthesized actively during the growth phase compared with the stationary phase in normal human epidermal keratinocytes in vitro. Intracellular zinc accumulation was observed in the proliferating cells. We concluded that hepatic MT plays an important role as an acute phase protein against host damage, and epidermal MT contributes in the supply of zinc to wounded tissue and activates proliferation for the regeneration of epidermis.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Zinc accumulation and metallothionein gene expression in the proliferating epidermis during wound healing in mouse skin

Iwata

Takebayashi

Ohta

et al. 1999

Histochemistry and Cell Biology

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“…Apparently the low plasma zinc is related to dietary factors other than zinc content. The repeated endotoxin exposures-the last injection was 5 d before dissectionalso failed to cause any accumulation of zinc, which would reflect induction of metallothionein synthesis in the liver, for example, as has been reported under these conditions (14,15). This may be due to the fact that hunger during pair-feeding also induces metallothionein synthesis, or to the fact that the increase in metallothionein is transient (1 6, 17).…”

Section: Discussionmentioning

confidence: 93%

Discrimination between Low Dietary Zinc and Endotoxin Exposure: A Model Study on Weaning Rats

Wouwe

Veldhuizen²,

Hamer³

et al. 1990

Pediatr Res

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ABSTRACT. To establish a parameter for zinc status that is independent of the occurrence of infection, we studied the effects of low dietary zinc and endotoxin in weaning rats 21 d after 65Zn intubation. We monitored aspects of zinc status (tissue zinc content, 65Zn distribution, and specific 6sZn activity in tissue) and 65Zn metabolism (absorption, excretion, and biologic half-life), as well as weight gain, feed conversion, and dietary zinc use. The low zinc diet induced classical deficiency with losses of bone zinc, resulting in lower content (7.4 versus 19.6 pmol) and higher spec act (17 versus 8 kBqlpmo1). Other tissue-specific and plasma-specific activities were also higher (overall, 20 versus 8 kBq/pmol; plasma, 8 versus 4 kBq/pmol). Endotoxin caused lower total-plasma zinc (0.04 versus 0.05 pmol) but did not affect spec act (4 kBq/pmol); combined endotoxin and low-zinc diet caused low total-plasma zinc (0.01 pmol) and high spec act, as did the low-zinc diet alone (12 kBq/pmol). We conclude that plasma-spec act (or stable isotope enrichment) can serve as an index for nutritional zinc status during recurrent infection. (Pediatr Res 28: 332-335,1990) Groups of children suffering from recurrent upper respiratory tract infection were found to have low hair zinc (1) and high urinary zinc values, as well as lower SD scores of height for age (2). However, these parameters are considered poor indicators of the zinc status of individual patients (3). Moreover, the relationship between zinc status and the health of a child is ambiguous: deficiency either predisposes to recurrent infection (4) or is induced by it (5). In this study involving young male rats, we monitored the zinc status as affected by low dietary zinc and repeated endotoxin exposure. Weight gain, efficiency of feed conversion, and dietary zinc use were measured. Zinc status was characterized by tissue zinc contents, tissue 65Zn distribution, and specific 65Zn activities (65Zn activity per mol zinc). Regulation of the zinc status was measured by whole-body 65Zn absorption, excretion, and turnover. On the basis of these results, we evaluated the suitability of the parameters studied to discriminate between low zinc intake, endotoxin exposure, and their combination.

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“…MTs are constitutively expressed in the liver and are overexpressed in endotoxemia (Kille et al, 1994;Abe et al, 1987;Hur et al, 1999). As one-third of its aminoacid residues are cysteines, MT provides a neutrophilic sink binding electrophiles (Kille et al, 1994;Kojima et al, 1976).…”

Section: Introductionmentioning

confidence: 99%

Effects of Kupffer cell blockade on the hepatic expression of metallothionein and heme oxygenase genes in endotoxemic rats with obstructive jaundice

et al. 2012

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Metallothionein and Zinc Metabolism in Endotoxin Shock Rats

Cited by 12 publications

References 10 publications

Zinc accumulation and metallothionein gene expression in the proliferating epidermis during wound healing in mouse skin

Zinc accumulation and metallothionein gene expression in the proliferating epidermis during wound healing in mouse skin

Discrimination between Low Dietary Zinc and Endotoxin Exposure: A Model Study on Weaning Rats

Effects of Kupffer cell blockade on the hepatic expression of metallothionein and heme oxygenase genes in endotoxemic rats with obstructive jaundice

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