Metallothionein deficiency in the injured peripheral nerves of complex regional pain syndrome as revealed by proteomics

Oki, Gosuke; Wada, Takuro; Iba, Kosuke; Aiki, Hikono; Sasaki, Kouichi; Imai, Shoichi; Sohma, Hitoshi; Matsumoto, Kayo; Yamaguchi, M.; Fujimiya, Mineko; Yamashita, Toshihiko; Kokai, Yasuo

doi:10.1016/j.pain.2011.11.008

Cited by 29 publications

(22 citation statements)

References 46 publications

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“…The proteome of the cerebrospinal fluid in healthy subjects, in patients with herniated disc and idiopathic back pain have been investigated [14], [15], [16]. Also, nerve samples from patients with complex regional pain syndrome (CRPS) have been analyzed in order to detect up/down regulated proteins [17]. Proteomics of serum have been used for the identification of possible biomarkers of chronic endometriosis [18], [19].…”

Section: Introductionmentioning

confidence: 99%

Multivariate Modeling of Proteins Related to Trapezius Myalgia, a Comparative Study of Female Cleaners with or without Pain

et al. 2013

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The prevalence of chronic trapezius myalgia is high in women with high exposure to awkward working positions, repetitive movements and movements with high precision demands. The mechanisms behind chronic trapezius myalgia are not fully understood. The purpose of this study was to explore the differences in protein content between healthy and myalgic trapezius muscle using proteomics. Muscle biopsies from 12 female cleaners with work-related trapezius myalgia and 12 pain free female cleaners were obtained from the descending part of the trapezius. Proteins were separated with two-dimensional differential gel electrophoresis (2D-DIGE) and selected proteins were identified with mass spectrometry. In order to discriminate the two groups, quantified proteins were fitted to a multivariate analysis: partial least square discriminate analysis. The model separated 28 unique proteins which were related to glycolysis, the tricaboxylic acid cycle, to the contractile apparatus, the cytoskeleton and to acute response proteins. The results suggest altered metabolism, a higher abundance of proteins related to inflammation in myalgic cleaners compared to healthy, and a possible alteration of the contractile apparatus. This explorative proteomic screening of proteins related to chronic pain in the trapezius muscle provides new important aspects of the pathophysiology behind chronic trapezius myalgia.

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Section: Introductionmentioning

confidence: 99%

Multivariate Modeling of Proteins Related to Trapezius Myalgia, a Comparative Study of Female Cleaners with or without Pain

et al. 2013

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“…The proteome of the cerebrospinal fluid in healthy subjects, in patients with herniated disc and idiopathic back pain have been studied [17]–[19]. Also, nerve samples from patients with complex regional pain syndrome (CRPS) have been analyzed using proteomics in order to detect up/down regulated proteins [20]. Proteomics of serum have been used for the identification of possible biomarkers of chronic endometriosis [21], [22].…”

Section: Introductionmentioning

confidence: 99%

Identification of Proteins from Interstitium of Trapezius Muscle in Women with Chronic Myalgia Using Microdialysis in Combination with Proteomics

Olausson¹,

Gerdle²,

Ghafouri

et al. 2012

PLoS ONE

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BackgroundMicrodialysis (MD) of the trapezius muscle has been an attractive technique to investigating small molecules and metabolites in chronic musculoskeletal pain in human. Large biomolecules such as proteins also cross the dialysis membrane of the catheters. In this study we have applied in vivo MD in combination with two dimensional gel electrophoresis (2-DE) and mass spectrometry to identify proteins in the extracellular fluid of the trapezius muscle.Materials and MethodsDialysate from women with chronic trapezius myalgia (TM; n = 37), women with chronic wide spread pain (CWP; n = 18) and healthy controls (CON; n = 22) was collected from the trapezius muscle using a catheter with a cut-off point of 100 kDa. Proteins were separated by two-dimensional gel electrophoresis and visualized by silver staining. Detected proteins were identified by nano liquid chromatography in combination with tandem mass spectrometry.ResultsNinety-seven protein spots were identified from the interstitial fluid of the trapezius muscle; 48 proteins in TM and 30 proteins in CWP had concentrations at least two-fold higher or lower than in CON. The identified proteins pertain to several functional classes, e.g., proteins involved in inflammatory responses. Several of the identified proteins are known to be involved in processes of pain such as: creatine kinase, nerve growth factor, carbonic anhydrase, myoglobin, fatty acid binding protein and actin aortic smooth muscle.ConclusionsIn this study, by using in vivo microdialysis in combination with proteomics a large number of proteins in muscle interstitium have been identified. Several of the identified proteins were at least two-fold higher or lower in chronic pain patients. The applied techniques open up for the possibility of investigating protein changes associated with nociceptive processes of chronic myalgia.

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“…This normally limits researchers to animal experiments. However, Oki et al performed a proteomic study of resected nerves in CRPS patients [25]. This study linked a deficiency of methallothionein in injured nerves with the syndrome.…”

Section: Cns Proteome In Pain Modelsmentioning

confidence: 99%

The Proteomics of Intrathecal Analgesic agents for Chronic Pain

Moore¹,

McCrory

2017

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Chronic pain remains a challenging clinical problem with a growing socio-economic burden for the state. Its prevalence is high and many of the patients are of work age. Our knowledge regarding the pathophysiology of chronic pain is poor. The consensus view is that the central nervous system plays a key role in the persistence of pain after an initiating event has long ceased. However the specifics of this biological response to an initiating event remains unclear. There is a growing body of evidence to support the concept that a central neuroimmune response is initiated and a number of small peptides have been implicated in this process following cerebrospinal fluid analysis in patients with chronic pain. This central biosynthetic peptide response leads to a process called central sensitization. Therapy is aimed at modulating and even inhibiting this response. However current pharmacological therapeutic options are limited in efficacy with significant deleterious side effect profiles. Proteomic studies extend single molecule analysis by identifying the components of biological networks and pathways and defining their interactions. This tool offers the potential to provide a molecular overview of the biological processes involved in chronic pain. It will also facilitate examination of gene-drug interactions. This technique offers a mechanism of defining the central biological responses that result in chronic pain and this information may facilitate the development of better therapies.

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Metallothionein deficiency in the injured peripheral nerves of complex regional pain syndrome as revealed by proteomics

Cited by 29 publications

References 46 publications

Multivariate Modeling of Proteins Related to Trapezius Myalgia, a Comparative Study of Female Cleaners with or without Pain

Multivariate Modeling of Proteins Related to Trapezius Myalgia, a Comparative Study of Female Cleaners with or without Pain

Identification of Proteins from Interstitium of Trapezius Muscle in Women with Chronic Myalgia Using Microdialysis in Combination with Proteomics

The Proteomics of Intrathecal Analgesic agents for Chronic Pain

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