Metallothionein Expression and Tumor Growth in the Transplantable Pregnancy-Independent Mouse Mammary Tumor

Abstract

ABSTRACT. In various human tumors, a metal binding protein, metallothionein (MT) is reported to play an important role in carcinogenesis. In the present preliminary study, MT expression and tumor growth were investigated in transplantable pregnancy-independent mammary tumors (TPIMT) derived from pregnancy-independent mammary tumors (PIMT) in GR/A mice, in order to study the possible role of MT in mammary carcinogenesis. TPIMT as well as PIMT showed MT expression in tumor cells in all of the successive transplantations. A negative correlation was observed between MT expression in transplanted tumor tissues and their growth in the hosts (r = -0 .53, p<0.05). The present study indicates that MT is a useful marker of tumor progression in TPIMT. KEY WORDS: metallothionein, progression, transplantable pregnancy-independent mouse mammary tumor.

Embryo developmental capability and pregnancy outcome are related to the mitochondrial DNA copy number and ooplasmic volume

“…The long axis (D) and minor axis (d) of each blastomere were measured under an inverted microscope. We calculated the blastomere volume using (πd 2 D÷6, π=3.14) [30]. Under an inverted microscope the blastomeres were rinsed in a drop of PBS and transferred into individual PCR tubes containing cell lysis solution.…”

Efeito inibitório da Curcumina na iniciação da carcinogênese colônica experimental

“…Possivelmente, o aumento da taxa de apoptose em animais administrados com curcumina pode estar relacionado ao aumento da expressão de MT, conforme observado em tumores mamários em camundongos (Yamamura et al, 2001). No entanto, os mecanismos de modulação e eventual correlação das expressões de MT, c-fos e SOD pela curcumina podem ser estudados em experimentos futuros.…”