Metallothionein induction and antioxidative responses in the estuarine polychaeta Capitella capitata (Capitellidae)

Suriya, J.; Bharathiraja, Subramaniyan; Sekar, Vaithilingam; Rajasekaran, R.

doi:10.1016/s2221-1691(12)60360-8

Cited by 24 publications

(8 citation statements)

References 30 publications

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“…It has been suggested that the removal of zinc from the sperm cell surface destabilizes the plasma membrane, playing an important role in preparation for the completion of capacitation and the acrosome reaction. Some studies have reported that zinc supplementation can also improve the synthesis of metallothioneins (low-molecular-weight Zn-binding proteins), which have properties of enhancing the quality of seminal fluids to protect sperm against damage 54 ; metallothioneins have the property of protecting biological tissues from the damage of oxidative stress via the capture of harmful oxidant species, such as superoxide and hydroxyl radicals 55 . Second, zinc in seminal plasma is involved in maintaining the stability of sperm chromatin 56 .…”

Section: Discussionmentioning

confidence: 99%

Zinc levels in seminal plasma and their correlation with male infertility: A systematic review and meta-analysis

Zhao

Dong

et al. 2016

Sci Rep

154

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Zinc is an essential trace mineral for the normal functioning of the male reproductive system. Current studies have investigated the relationship between seminal plasma zinc and male infertility but have shown inconsistent results. Hence, we systematically searched PubMed, EMBASE, Science Direct/Elsevier, CNKI and the Cochrane Library for studies that examined the relationship between seminal plasma zinc and male infertility, as well as the effects of zinc supplementation on sperm parameters. Twenty studies were identified, including 2,600 cases and 867 controls. Our meta-analysis results indicated that the seminal plasma zinc concentrations from infertile males were significantly lower than those from normal controls (SMD (standard mean differences) [95% CI] −0.64 [−1.01, −0.28]). Zinc supplementation was found to significantly increase the semen volume, sperm motility and the percentage of normal sperm morphology (SMD [95% CI]: −0.99 [−1.60, −0.38], −1.82 [−2.63, −1.01], and −0.75 [−1.37, −0.14], respectively). The present study showed that the zinc level in the seminal plasma of infertile males was significantly lower than that of normal males. Zinc supplementation could significantly increase the sperm quality of infertile males. However, further studies are needed to better elucidate the correlation between seminal plasma zinc and male infertility.

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Section: Discussionmentioning

confidence: 99%

Zinc levels in seminal plasma and their correlation with male infertility: A systematic review and meta-analysis

Zhao

Dong

et al. 2016

Sci Rep

154

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“…The results of the study indicated that zinc supplementation improves the synthesis of zinc binding proteins such as metallothioneins in seminal plasma of asthenozoospermic subjects [ 33 ]. Metallothioneins protect biological tissues from damage of oxidative stress via capture harmful oxidant species like the superoxide and hydroxyl radicals [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Study of the effects of oral zinc supplementation on peroxynitrite levels, arginase activity and NO synthase activity in seminal plasma of Iraqi asthenospermic patients

Hadwan

Almashhedy

Alsalman

2014

Reprod Biol Endocrinol

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BackgroundLow concentrations of nitric oxide (NO) are necessary for the biology and physiology of spermatozoa, but high levels of NO are toxic and have negative effects on sperm functions. Although several studies have considered the relationship between infertility and semen NO concentrations, no study on the effects of asthenospermia treatments such as oral zinc supplementation on concentrations of NO, which are important in fertility, has been reported. Studies have shown that oral zinc supplementation develops sperm count, motility and the physical characteristics of sperm in animals and in some groups of infertile men. The present study was conducted to study the effect of zinc supplementation on the quantitative and qualitative characteristics of semen, along with enzymes of the NO pathway in the seminal plasma of asthenospermic patients.MethodsSemen samples were obtained from 60 fertile and 60 asthenozoospermic infertile men of matched age. The subfertile group was treated with zinc sulfate; each participant took two capsules (220 mg per capsule) per day for 3 months. Semen samples were obtained (before and after zinc sulfate supplementation). After liquefaction of the seminal fluid at room temperature, routine semen analyses were performed. The stable metabolites of NO (nitrite) in seminal plasma were measured by nitrophenol assay. Arginase activity and NO synthase activity were measured spectrophotometrically.ResultsPeroxynitrite levels, arginase activity, NO synthase activity and various sperm parameters were compared among fertile controls and infertile patients (before and after treatment with zinc sulfate). Peroxynitrite levels and NO synthase activity were significantly higher in the infertile patients compared to the fertile group. Conversely, arginase activity was significantly higher in the fertile group than the infertile patients. Peroxynitrite levels, arginase activity and NO synthase activity of the infertile patient were restored to normal values after treatment with zinc sulfate. Volume of semen, progressive sperm motility percentage and total normal sperm count were increased after zinc supplementation.ConclusionsTreatment of asthenospermic patients with zinc supplementation leads to restored peroxynitrite levels, arginase activity and NO synthase activity to normal values and gives a statistically significant improvement of semen parameters compared with controls.Trial registrationClinicalTrials.gov identifier: NCT01684059

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“…For MT activity determination, we added 0.1 mmol phenylmethylsulfonyl fluoride (PMSF) as antiprotease agent and 0.5 mmol dithiothreitol (DTT) as the sulfydryl-protecting agent to prevent the MT from oxidation and 1 mL of the supernatant was heated at 100°C for 10 min in order to precipitate non-MT protein and later cooled on ice for 5 min. We fractionated aliquots of the heat-treated homogenate by gel-permeation chromatography [Sephadex G 75 (26 mm by 60 cm)] and the MT concentration in the samples was estimated with the mercury-saturation assay described by Suriya et al (2012). LDH activity was determined according to the method of Vassault (1983) where 2.5 mL of a TRIS/NaCl/NADH solution and 0.5 mL of pyruvate solution were added to the homogenate at 340 nm every 30 s for 3 min and the values were expressed as nmols of reduced pyruvate/min/mg protein.…”

Section: Biochemical Enzyme Analysismentioning

confidence: 99%

Biochemical response and vermiremediation assessment of three earthworm species (Alma millsoni, Eudrilus eugeniae and Libyodrilus violaceus) in soil contaminated with a glyphosate-based herbicide

Owagboriaye

Dedeke

Bamidele

et al. 2020

Ecological Indicators

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The global use of glyphosate-based herbicides (GBHs) and release of glyphosate residues in soil environment have over the years been a major concern. In this study, we aim to evaluate the biochemical response and vermiremediation potential of three indigenous earthworm species; namely Alma millsoni, Eudrilus eugeniae and Libyodrilus violaceus in GBH treated soils. Study design: Three weed plants (Tridax procumbense, Ludwigia pasturis and Pannicum maximum) were transplanted into 140 plastic pots and 20 adult individual earthworms of each species were later introduced into 60 pots. In total, each earthworm species treatments representing 20 pots and 20 pots without earthworm were sprayed with 115.49 mL/m 2 (equivalent to 83.2 g a.i/m 2) of Roundup® Alphée. The remaining 60 pots with earthworm species were left unsprayed. Activities of glutathione-S-transferase (GST), lactate dehydrogenase (LDH), metallothionine (MT), acetylcholinesterase (AChE), antioxidant defense system and lipid peroxidation (MDA) were monitored at the 1st, 2nd, 4th, 6th and 8th weeks Post Herbicide Application (PHA) using standard methods. Glyphosate residues in the soil and earthworm species were quantified with a High-Performance Liquid Chromatography (HPLC) with fluorescence detector. Bioaccumulation Factor (BAF) was also calculated. Results: Higher activities of GST and LDH and reduction in MT activities were observed in the three earthworm species exposed to GBH compared to the unexposed while AChE activity was insensitive to the herbicide. The antioxidant defence system was able to protect E. eugeniae and L. violaceus against oxidative stress. The presence of earthworms reduced glyphosate residues in the soil. E. eugeniae and L. violaceus were bioaccumalators and biomagnifiers of glyphosate as indicated by the BAF (> 1) obtained after 8th week PHA. Relationships exist between glyphosate BAF in E. eugeniae and L. violaceus and exposure duration as well as between glyphosate residues in tissues of the earthworm species and their biochemical parameters. Conclusions: Alterations in the enzymatic activities and antioxidant defence of the earthworm species could be an index for GBH contaminated soil monitoring and assessment; both E. eugeniae and L. violaceus showed potential to vermiremediate soils contaminated with GBH.

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Metallothionein induction and antioxidative responses in the estuarine polychaeta Capitella capitata (Capitellidae)

Cited by 24 publications

References 30 publications

Zinc levels in seminal plasma and their correlation with male infertility: A systematic review and meta-analysis

Zinc levels in seminal plasma and their correlation with male infertility: A systematic review and meta-analysis

Study of the effects of oral zinc supplementation on peroxynitrite levels, arginase activity and NO synthase activity in seminal plasma of Iraqi asthenospermic patients

Biochemical response and vermiremediation assessment of three earthworm species (Alma millsoni, Eudrilus eugeniae and Libyodrilus violaceus) in soil contaminated with a glyphosate-based herbicide

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