Metallothionein-Like Proteins and Energy Reserve Levels after Ni and Pb Exposure in the Pacific White PrawnPenaeus vannamei

Abstract

This study analyzed the changes in metallothionein-like proteins (MTLPs) and Energy Reserves (ERs) in hepatopancreas and abdominal muscle of the white prawn Penaeus vannamei. Realistic metal concentration exposure for 10 days to Ni and Pb in solution revealed that juvenile prawns partially induce MTLP in hepatopancreas after Pb exposure. Ni was distributed equally between soluble and insoluble fractions, while Pb was present only in the insoluble fraction, suggesting different detoxification strategy. No changes in lipids and glycogen concentration were detected under these experimental conditions in both tissues analyzed. MTLP could not be considered as a suitable indicator for lead exposure in hepatopancreas.

Implications on the Pb bioaccumulation and metallothionein levels due to dietary and waterborne exposures: The Callinectes danae case

“…In Penaeus monodon shrimp hepatopancreas, Vogt and Quinitio (1994) suggested that Pb is aggregated as deposits (or granules) of nonsoluble Pb and excreted after lysosomal autolysis, a process that may involve MT. Núñez-Nogueira et al, (2010 suggested a partial Pb regulation by the Penaeus vannamei shrimp, since most Pb was detected as non-soluble deposits in hepatopancreas. Rainbow (1998) pointed out that isopods accumulate metals in granules, with rapid Pb elimination in comparison to other metals.…”

A baseline study of the metallothioneins content in digestive gland of the Norway lobster Nephrops norvegicus from Northern Adriatic Sea: Body size, season, gender and metal specific variability

“…Previous laboratory and field studies demonstrated positive correlation of Pb, Cr and Mn to MTs content in some crustaceans (Lavadras et al, 2014;Martín-Díaz et al, 2009). On the other hand, at exposure concentrations of Pb relevant for contaminated marine environment, the accumulation of Pb in digestive gland tissue of shrimp L. vannamei was not observed and could not be linked to MTs content increase (Nunez-Nogueira et al, 2010). Hence, the lack of correlation to MTs could be explained by relatively low concentrations of Pb that were two to tenfold lower than values previously reported by Canli and Furness (1993a) for digestive gland of N. norvegicus, and within values for other crustaceans from low to moderately contaminated marine areas (Canli et al, 2001;Leblanc and Prince, 2012;Lewtas et al, 2014;Yilmaz and Yilmaz, 2007).…”

“…In addition to the metal-detoxifying role, crustaceans MTs are also involved in protection from reactive oxygen species (ROS) that can cause damage to cell macromolecules when produced in excessive quantities (Pan and Zhang, 2006;Moltó et al, 2007;Lobato et al, 2013;Felix-Portillo et al, 2014). Metals can be distributed into soluble cytosol, or prevail within insoluble fraction that include metal-rich granules, involved in the second important metal detoxification pathway in crustaceans (Barka, 2007;Legras et al, 2000;Mouneyrac et al, 2001;Nunez-Nogueira et al, 2010).…”

EFECTO DE LIXIVIDADO DE MANGLAR EN LA TOXICIDAD DE NÍQUEL Y CADMIO EN EL CAMARÓN ESTUARINO (Hippolyte zostericola) DE LA LAGUNA DE TÉRMINOS, CAMPECHE

“…Analizando de manera individual cada elemento metálico, sabemos que biológicamente, uno se comporta de manera esencial (Ni) y el otro de manera no esencial (Cd) en camarones, al menos como lo demuestran algunos estudios previos en el camarón blanco del Pacífico ('Litopenaeus vannamei'), (Núñez-Nogueira et al, 2009;Nunez-Nogueira et al, 2010). Dicho comportamiento, aquí se comprueba, ya que el cadmio resulto ser más toxico que el níquel.…”