Metallothioneins and lysosomes in metal toxicity and accumulation in marine mussels: the effect of cadmium in the presence and absence of phenanthrene

Viarengo, Aldo; Moore, MN; Mancinelli, G.; Mazzucotelli, A.; Pipe, R.K.; Farrar, Suzanne V.

doi:10.1007/bf00392937

Cited by 135 publications

(34 citation statements)

References 15 publications

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“…Enhanced accumulation of neutral lipids and lipofuscin has reportedly been observed in secondary lysosomes and digestive cells of bivalves exposed to organic and toxic and essential elemental contaminants , Viarengo et al 1987, Moore 1988, Krishnakumar et al 1990, Regoli 1992). Capuzzo & Leavitt (1988) and Leavitt et al (1990) have similarly reported that an increase in the biochemically determined amount of neutral lipids with a concomitant decrease in polar lipid content occurs in the digestive gland cells of contaminant-exposed bivalves.…”

mentioning

confidence: 99%

Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA. I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis

Krishnakumar¹,

Casillas²,

Varanasi³

1994

Mar. Ecol. Prog. Ser.

132

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Lysosomal responses in the digestive cells of Mytilus eduljs from 9 sites in Puget Sound, Washington. USA, with varying degrees of sediment contamination were measured using automatic image analysis. Mussels from urban-associated sltes (areas with elevated sediment concentration of anthropogenic contaminants such as PAHs, PCBs. DDTs, and toxic elements) showed pathological changes in the lysosomes (reduced lysosomal labilization period, reduced lysosomal stability and increased N-acetyl P-hexosaminidase activity), enhanced lipofuscin deposition and increased accumulat~on of lysosomal and cytoplasmic unsaturated neutral lipids. Morphometric measures of growth showed that mussels were generally smaller in size and that somatic tissue weight relative to shell length was lower in mussels from urban-associated sites relative to those from minimally contaminated sites. Highly significant correlations were observed between concentrations of tissue PAH, PCB, and selected toxic elements (measures of anthropogenic exposure) and lysosomal labil~zation period. Pathological alterations in the lysosomal system of mussels from the urban-associated sites were probably induced by the high tissue concentrations of organic and inorganic contaminants rather than as a result of potential confounding biotic (spawning stress) or abiotic (temperature, salinity and food availability) factors. The results show that cytochemical measurement of lysosomal responses combined with automatic image analysis have the potential to be used as sensitive, accurate and rapid techniques for assessing the biological impact of environmental contaminants in mussels in the near coastal environment.

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confidence: 99%

Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA. I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis

Krishnakumar¹,

Casillas²,

Varanasi³

1994

Mar. Ecol. Prog. Ser.

132

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“…In many invertebrates, lysosomes play a key role in the process of mediating enzymes for the catabolism of endogenous and exogenous material, and in the regulation of the cellular homeostasis, by fagocitosis, digestion, depuration and excretion of xenobiotics (MOORE, 1985;VIARENGO et al, 1987;MCVEIGH at al., 2006). As contaminants accumulate in the lysosomes, alterations and damages in the lysosomal membranes may occur and cause a releasing of the digestive enzymes to the citosol, which would produce autolysis (MOORE, 1985;MCVEIGH et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

PHYSIOLOGICAL RESPONSES OF THE BROWN MUSSEL Perna perna (MOLLUSCA, BIVALVIA) EXPOSED TO THE ANIONIC SURFACTANT LINEAR ALKYLBENZENE SULPHONATE (LAS)

Stefanoni

Abessa

2011

RHE

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The effects of the Linear Alkylbenzene Sulphonate (LAS) were evaluated on the mussel Perna perna, using physiological and genotoxic biomarkers. The Micronuclei (MN) assay was used to estimate effects at nuclear level, whereas the physiological effects were evaluated by measuring the oxygen consumption and ammonia excretion rates. Significant effects were observed for the MN assay and the ammonia excretion rate, even in low concentrations. The oxygen consumption was not affected in the tested concentrations. For MN and ammonia excretion, the animals exposed to intermediate concentrations were not affected, but responded to the higher concentrations, indicating the existence of compensatory mechanisms at physiological level. However, parallel to this study other authors indicate the presence of progressive effects at the cellular level, suggesting that the organisms are not capable to recover of such increasing effects. Additionally, the results show that the levels of LAS observed for Brazilian coastal waters may chronically affect the biota. Keywords: Ecotoxicology. Biomarkers. Detergent. Effect. Marine pollution. Mussel. RESUMOOs efeitos do Alquilbenzeno Sulfonato de Sódio Linear sobre o mexilhão Perna perna foram avaliados por meio do uso de biomarcadores genotóxicos e fisiológicos. O ensaio da taxa de micronúcleos (MN) foi conduzido para estimar o efeito ao nível nuclear, enquanto os efeitos fisiológicos foram avaliados através da medição da taxa de consumo de oxigênio e de excreção de amônia. Efeitos significativos foram observados para o ensaio do MN e para a taxa de excreção de amônia, mesmo em baixas concentrações. O consumo do oxigênio não foi afetado pelas HOLOS Environment, v.11 n.1, 2011 -P. 45 ISSN:1519 concentrações dos ensaios. Para MN e excreção de amônia, os animais expostos às concentrações intermediárias não foram afetados, mas responderam às maiores concentrações, sugerindo a existência de mecanismos compensatórios em nível fisiológico. Contudo, paralelamente ao presente estudo, outros autores demonstraram haver efeitos progressivos em nível celular, sugerindo que os organismos não conseguem se recuperar dos efeitos crescentes da exposição. Os resultados ainda mostram que níveis de LAS presentes na costa brasileira podem afetar cronicamente a biota. Palavras-chave: Ecotoxicologia. Biomarcadores. Detergente. Efeito. Poluição marinha. Mexilhão.

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“…This can destabilise the lysosomal membrane causing the contents to leak out into the 550 cytosol thereby reducing the cells capacity to remove waste which will ultimately lead to cell 551 death (Viarengo et al, 1987). Similar to T. deltoidalis exposed to cadmium and lead (Taylor 552 and Maher, 2013;2014), the selenium exposed T. deltoidalis had significantly higher 553 lysosomal destabilisation than the control organisms ( Figure 4B).…”

Section: Cellular Biomarker -Lysosomal Stability 547mentioning

confidence: 97%

Exposure-dose-response of Tellina deltoidalis to contaminated estuarine sediments 3. Selenium spiked sediments

Taylor

Maher

2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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Metallothioneins and lysosomes in metal toxicity and accumulation in marine mussels: the effect of cadmium in the presence and absence of phenanthrene

Cited by 135 publications

References 15 publications

Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA. I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis

Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA. I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis

PHYSIOLOGICAL RESPONSES OF THE BROWN MUSSEL Perna perna (MOLLUSCA, BIVALVIA) EXPOSED TO THE ANIONIC SURFACTANT LINEAR ALKYLBENZENE SULPHONATE (LAS)

Exposure-dose-response of Tellina deltoidalis to contaminated estuarine sediments 3. Selenium spiked sediments

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