Metallothioneins and zinc in cancer diagnosis and therapy

Křížková, Soňa; Ryvolová, Markéta; Hraběta, Jan; Adam, Vojtěch; Stiborová, Marie; Eckschlager, Tomáš; Kizek, René

doi:10.3109/03602532.2012.725414

Cited by 82 publications

(63 citation statements)

References 210 publications

(200 reference statements)

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“…4, all four measured indicators amounted to the highest value in fibropapillomas. The role of MTs and zinc in cancer development is closely linked and the structure and function of MTs depend strongly on the redox state of zinc and its interactions with proteins (Krizkova et al 2012). A similar increase in the concentration of MTs has previously been documented in skin melanoma cells both in vivo and in vitro (Krizkova et al 2008).…”

Section: Discussionsupporting

confidence: 49%

Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

et al. 2015

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The present study aimed at the aetiological diagnosis of skin tumours of roe deer (Capreolus capreolus) in the Czech Republic. A total of 33 roe deer specimens showing skin masses were sampled for histopathology, virus detection and identification using polymerase chain reaction (PCR) and deoxyribonucleic acid sequence (DNA) analysis, and to investigate fibropapillomaassociated levels of metallothionein, zinc and oxidative stress in 2012 and 2013. Ticks (Ixodes ricinus) and deer keds (Lipoptena cervi) were also collected and pooled from sampled game specimens. Ticks found on dogs in hunting grounds under study were pooled into another sample. Skin tumours, ranging from 2 to 10 per inspected specimen and 2 to 5 cm in size, were classified as fibropapillomas by histopathology. All 33 viral-infection-suspected skin samples from roe deer in South Moravia and South Bohemia were PCR positive. The nucleotide sequences of PCR products were 100% homologous to the Western roe deer papillomavirus 1 isolate CcPV-1. Ticks and deer keds from positive roe deer and ticks from dogs were PCR and DNA sequence positive for the roe deer papillomavirus. Viral DNA was also demonstrated in one blood sample from a roe deer female. Differences in metallothionein, zinc, taurine and electrochemical index among samples from lesions and normal skin of affected roe deer and negative controls from papillomavirus-non-infected animals were non-significant. While we have demonstrated circulation of specific roe deer papillomavirus in the Czech Republic that results in multiple fibropapillomatous skin tumours, many ecological and epidemiological issues of this wildlife disease still remain unanswered.

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Section: Discussionsupporting

confidence: 49%

Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

et al. 2015

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“…At the protein level, MT has been examined as a marker of cancer [29] and also of metals' and toxic compounds' intoxication [30,31]. For these purposes, numerous analytical and biochemical methods are used [32,33], whereas the electrochemical ones seem to be the most sensitive [33][34][35].…”

Section: Metallothionein Expressionmentioning

confidence: 99%

Microarray analysis of metallothioneins in human diseases—A review

Křížková

Kępińska

Emri

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1 Microarray analysis of metallothioneins in human diseases AbstractMetallothioneins (MTs), low molecular mass cysteine-rich proteins, which are able to bind up to 20 monovalent and up to 7 divalent heavy metal ions are widely studied due to their functions in detoxification of metals, scavenging free radicals and cells protection against the oxidative stress. It was found that the loss of the protective effects of MT leads to an escalation of pathogenic processes and carcinogenesis. 3The most extensive area is MTs expression for oncological applications, where the information about gene patterns is helpful for the identification biological function, resistance to drugs and creating the correct chemotherapy. In other medical applications the effect of oxidative stress to cell lines exposed to heavy metals and hydrogen peroxide is studied as well as influence of drugs and cytokines on MTs expression and MTs expression in the adiposetissue. The precise detection of low metallothionein concentrations and its isoforms is necessary to understand the connection between quantity and isoforms of MTs to size, localization and type of cancer. This information is necessary for well-timed therapy and increase the chance to survival. Microarray chips appear as good possibility for finding all information about expression of MTs genes and isoforms not only in cancer, but also in other diseases, especially diabetes, obesity, cardiovascular diseases, ageing, osteoporosis, psychiatric disorders and as the effects of toxic drugs and pollutants, which is discussed in this review. List of abbreviations:HCC -hepatocellular carcinoma, HFD -high fat diet, MMP -matrix metalloproteinase, MT -metallothionein, MTF -metal transcription factor, MRE -metal responsive element, ROS -reactive oxygen species.

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“…MTs are widely distributed in invertebrates and vertebrates, plants, prokaryotes and even in fungi kingdoms [4][5][6][7]. Despite the diversity of the amino acid sequences, the MTs are generally analogous in structure with respect to a high content of cysteine residues (up to 30%), and lack of aromatic amino acids [4].…”

Section: Introductionmentioning

confidence: 99%

“…Despite the diversity of the amino acid sequences, the MTs are generally analogous in structure with respect to a high content of cysteine residues (up to 30%), and lack of aromatic amino acids [4]. MTs are involved in the metabolism of heavy metal ions, including their metal detoxification, homeostasis [8,9], storage of zinc [7], radical scavenging [2,10] and stress response [11]. MTs are overexpressed in several tumors and this overexpression is accompanied by an increased proliferation and protection against apoptosis.…”

Section: Introductionmentioning

confidence: 99%