Metamorphosis and Gonad Maturation in the Horn Fly<i>Haematobia irritans</i>

Basso, Alicia; Forneris, Natalia Soledad; Filiberti, Adrian; Argaraña, Carlos E.; Rabossi, Alejandro; Quesada-Allué, Luis A.

doi:10.1673/031.011.17401

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…Initial production of spermatocytes and early spermatids generally begins in the larval stages in endopterygote insects [27,82]. Our experiments reveal vulnerability from the pupal stage, when the male reproductive system is active with spermiogenesis and the formation of spermatids with tails, mitochondria and nuclear condensation [83–85].…”

Section: Discussionmentioning

confidence: 99%

Fertility and mortality impacts of thermal stress from experimental heatwaves on different life stages and their recovery in a model insect

2021

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With climate change creating a more volatile atmosphere, heatwaves that create thermal stress for living systems will become stronger and more frequent. Using the flour beetle Tribolium castaneum , we measure the impacts of thermal stress from experimental heatwaves in the laboratory on reproduction and survival across different insect life stages, and the extent and pace of any recovery. We exposed larvae, pupae, juvenile and mature adult male beetles to 5-day periods of heat stress where temperatures were maintained at either 40°C or 42°C, a few degrees above the 35°C optimum for this species' population productivity, and then measured survival and reproduction compared with controls at 30°C. Mortality due to thermal stress was greatest among juvenile life stages. Male reproductive function was specifically damaged by high temperatures, especially if experienced through pupal or immature life stages when complete sterility was shown at reproductive maturity; larval exposure did not damage adult male fertility. High temperatures impaired testis development and the production of viable sperm, with damage being strongest when experienced during pupal or juvenile adult stages. Despite this disruption, males recovered from heat stress and, depending on the stage of exposure, testis size, sperm production and fertility returned to normal 15–28 days after exposure. Our experiments reveal how thermal stress from heatwave conditions could impact on insect survival and reproduction across different life stages, and the potential and timescales of recovery.

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Section: Discussionmentioning

confidence: 99%

Fertility and mortality impacts of thermal stress from experimental heatwaves on different life stages and their recovery in a model insect

2021

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“…Larval development took place in an environmental chamber at 29±1 °C. The life cycle of Haematobia irritans was partially described elsewhere (Basso et al 2011). …”

Section: Methodsmentioning

confidence: 99%

“…Testes of pharate adult males (120 h after puparium formation) and eclosed adults up to 36 h after emergence were used (Basso et al 2011). The tissues were fixed and stained in a drop of lacto-acetic 2% orcein solution, covered with a cover slip and squashed 10 minutes later.…”

Section: Methodsmentioning

confidence: 99%

High chromosomal variation in wild horn fly Haematobia irritans (Linnaeus) (Diptera, Muscidae) populations

Forneris¹,

Otero²,

Pereyra³

et al. 2015

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The horn fly, Haematobia irritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by Haematobia irritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of the horn fly increases our understanding of the phenotypes resistant to insecticides that repeatedly develop in these insects. The karyotype of Haematobia irritans, as previously described using flies from an inbred colony, shows a chromosome complement of 2n=10 without heterochromosomes (sex chromosomes). In this study, we analyze for the first time the chromosome structure and variation of four wild populations of Haematobia irritans recently established in the Southern Cone of South America, collected in Argentina and Uruguay. In these wild type populations, we confirmed and characterized the previously published “standard” karyotype of 2n=10 without sex chromosomes; however, surprisingly a supernumerary element, called B-chromosome, was found in about half of mitotic preparations. The existence of statistically significant karyotypic diversity was demonstrated through the application of orcein staining, C-banding and H-banding. This study represents the first discovery and characterization of horn fly karyotypes with 2n=11 (2n=10+B). All spermatocytes analyzed showed 5 chromosome bivalents, and therefore, 2n=10 without an extra chromosome. Study of mitotic divisions showed that some chromosomal rearrangements affecting karyotype structure are maintained as polymorphisms, and multiple correspondence analyses demonstrated that genetic variation was not associated with geographic distribution. Because it was never observed during male meiosis, we hypothesize that B-chromosome is preferentially transmitted by females and that it might be related to sex determination.

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Metamorphosis and Gonad Maturation in the Horn FlyHaematobia irritans

Cited by 2 publications

References 21 publications

Fertility and mortality impacts of thermal stress from experimental heatwaves on different life stages and their recovery in a model insect

Fertility and mortality impacts of thermal stress from experimental heatwaves on different life stages and their recovery in a model insect

High chromosomal variation in wild horn fly Haematobia irritans (Linnaeus) (Diptera, Muscidae) populations

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