Metamorphosis of <i>Galleria mellonella</i> research

Eisenman, Helene C.

doi:10.1080/21505594.2014.998541

Cited by 6 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hemocytes can discriminate between non-germinated, germinating and hyphal forms of A. fumigatus which is also the case with human neutrophils [ 25 , 26 ]. Aspergillus terreus infection in G. mellonella demonstrated unique histological findings consistent with those observed in disseminated aspergillosis in mammals [ 27 ].…”

Section: Introductionmentioning

confidence: 81%

Characterisation of the cellular and proteomic response of Galleria mellonella larvae to the development of invasive aspergillosis

2018

View full text Add to dashboard Cite

BackgroundGalleria mellonella larvae were infected with conidia of Aspergillus fumigatus and the cellular and humoral immune responses of larvae to the pathogen were characterized as invasive aspergillosis developed.ResultsAt 2 h post-infection there was an increase in hemocyte density to 7.43 ± 0.50 × 106/ml from 0.98 ± 0.08 × 106/ml at 0 h. Hemocytes from larvae immune primed for 6 h with heat killed A. fumigatus conidia displayed superior anti-fungal activity. Examination of the spread of the fungus by Cryo-imaging and fluorescent microscopy revealed dissemination of the fungus through the larvae by 6 h and the formation of distinct nodules in tissue. By 24 h a range of nodules were visible at the site of infection and at sites distant from that indicating invasion of tissue. Proteomic analysis of larvae infected with viable conidia for 6 h demonstrated an increase in the abundance of gustatory receptor candidate 25 (37 fold), gloverin-like protein (14 fold), cecropin-A (11 fold). At 24 h post-infection gustatory receptor candidate 25 (126 fold), moricin-like peptide D (33 fold) and muscle protein 20-like protein (12 fold) were increased in abundance. Proteins decreased in abundance included fibrohexamerin (13 fold) and dimeric dihydrodiol dehydrogenase (8 fold).ConclusionThe results presented here indicate that G. mellonella larvae may be a convenient model for studying the stages in the development of invasive aspergillosis and may offer an insight into this process in mammals.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1208-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 81%

Characterisation of the cellular and proteomic response of Galleria mellonella larvae to the development of invasive aspergillosis

2018

View full text Add to dashboard Cite

show abstract

“…Both species cause a hemocyte decrease and kill G. mellonella in a similar way. However, P. lutzii has higher adhesion ability to hemocytes and this could be attributed to the higher expression of the gp43 gene ( Scorzoni et al, 2015 ). To study the importance of the adhesins for the virulence of P. brasiliensis and P. lutzii , the treatment of Paracoccidioides spp.…”

Section: Advances In Animal Experimentation For the Study Of The Pcmmentioning

confidence: 99%

Paracoccidioides-host Interaction: An Overview on Recent Advances in the Paracoccidioidomycosis

et al. 2015

View full text Add to dashboard Cite

Paracoccidioides brasiliensis and P. lutzii are etiologic agents of paracoccidioidomycosis (PCM), an important endemic mycosis in Latin America. During its evolution, these fungi have developed characteristics and mechanisms that allow their growth in adverse conditions within their host through which they efficiently cause disease. This process is multi-factorial and involves host–pathogen interactions (adaptation, adhesion, and invasion), as well as fungal virulence and host immune response. In this review, we demonstrated the glycoproteins and polysaccharides network, which composes the cell wall of Paracoccidioides spp. These are important for the change of conidia or mycelial (26°C) to parasitic yeast (37°C). The morphological switch, a mechanism for the pathogen to adapt and thrive inside the host, is obligatory for the establishment of the infection and seems to be related to pathogenicity. For these fungi, one of the most important steps during the interaction with the host is the adhesion. Cell surface proteins called adhesins, responsible for the first contact with host cells, contribute to host colonization and invasion by mediating this process. These fungi also present the capacity to form biofilm and through which they may evade the host’s immune system. During infection, Paracoccidioides spp. can interact with different host cell types and has the ability to modulate the host’s adaptive and/or innate immune response. In addition, it participates and interferes in the coagulation system and phenomena like cytoskeletal rearrangement and apoptosis. In recent years, Paracoccidioides spp. have had their endemic areas expanding in correlation with the expansion of agriculture. In response, several studies were developed to understand the infection using in vitro and in vivo systems, including alternative non-mammal models. Moreover, new advances were made in treating these infections using both well-established and new antifungal agents. These included natural and/or derivate synthetic substances as well as vaccines, peptides, and anti-adhesins sera. Because of all the advances in the PCM study, this review has the objective to summarize all of the recent discoveries on Paracoccidioides-host interaction, with particular emphasis on fungi surface proteins (molecules that play a fundamental role in the adhesion and/or dissemination of the fungi to host-cells), as well as advances in the treatment of PCM with new and well-established antifungal agents and approaches.

show abstract

“…Part of this increased mortality results from the more complex and less effective antimicrobial treatments needed for multidrug-resistant infections. Virulence factor genes that ride with AMR genes on mobile genetic elements might contribute to the problem [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance: one world, one fight!

Harbarth

Balkhy

Goossens

et al. 2015

Antimicrob Resist Infect Control

199

128

View full text Add to dashboard Cite

The lack of new antibiotic classes calls for a cautious use of existing agents. Yet, every 10 min, almost two tons of antibiotics are used around the world, all too often without any prescription or control. The use, overuse and misuse of antibiotics select for resistance in numerous species of bacteria which then renders antimicrobial treatment ineffective. Almost all countries face increased antimicrobial resistance (AMR), not only in humans but also in livestock and along the food chain. The spread of AMR is fueled by growing human and animal populations, uncontrolled contamination of fresh water supplies, and increases in international travel, migration and trade. In this context of global concern, 68 international experts attending the fifth edition of the World HAI Resistance Forum in June 2015 shared their successes and failures in the global fight against AMR. They underlined the need for a “One Health” approach requiring research, surveillance, and interventions across human, veterinary, agricultural and environmental sectors. This strategy involves concerted actions on several fronts. Improved education and increased public awareness are a well-understood priority. Surveillance systems monitoring infections need to be expanded to include antimicrobial use, as well as the emergence and spread of AMR within clinical and environmental samples. Adherence to practices to prevent and control the spread of infections is mandatory to reduce the requirement of antimicrobials in general care and agriculture. Antibiotics need to be banned as growth promoters for farm animals in countries where it has not yet been done. Antimicrobial stewardship programmes in animal husbandry have proved to be efficient for minimising AMR, without compromising productivity. Regarding the use of antibiotics in humans, new tools to provide highly specific diagnoses of pathogens can decrease diagnostic uncertainty and improve clinical management. Finally, infection prevention and control measures – some of them as simple as hand hygiene – are essential and should be extended beyond healthcare settings. Aside from regulatory actions, all people can assist in AMR reduction by limiting antibiotic use for minor illnesses. Together, we can all work to reduce the burden of AMR.

show abstract

Metamorphosis of Galleria mellonella research

Cited by 6 publications

References 27 publications

Characterisation of the cellular and proteomic response of Galleria mellonella larvae to the development of invasive aspergillosis

Characterisation of the cellular and proteomic response of Galleria mellonella larvae to the development of invasive aspergillosis

Paracoccidioides-host Interaction: An Overview on Recent Advances in the Paracoccidioidomycosis

Antimicrobial resistance: one world, one fight!

Contact Info

Product

Resources

About