Metaproteomic insights into ammonia oxidising bacterial consortium developed for bioaugmenting nitrification in aquaculture systems

Jose, Divya; Preena, Prasannan Geetha; Kumar, V. J. Rejish; Philip, Rosamma; Singh, I. S. Bright

doi:10.2478/s11756-020-00481-3

Cited by 3 publications

(7 citation statements)

References 27 publications

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“…Meanwhile, Preena et al (2017) could successfully segregate the aerobic denitrifiers from the nitrifying bacterial consortia, which had been used for activating nitrifying bioreactor under aerobic conditions in recirculating aquaculture systems. Through metaproteomic approach, detected the production of denitrifying protein such as nosZ during effective nitrification of ammonia-oxidizing consortia and nitrate was not found to get accumulated in the system thereby ensuring effective denitrification (Kumar et al 2011;Jose et al 2020). Very recently, simultaneous partial nitrification, anammox and denitrification (SNAD) bioreactor was successfully demonstrated for the first time in RAS with high dissolved oxygen conditions and without any additional organic carbon supply (Lu et al 2020).…”

Section: Current Application Status and Prospects Of Nitrification An...mentioning

confidence: 99%

“…But this tool was also hardly utilized for the detection of nitrifying proteins expressed during the active N removal processes in RAS. However, we had successfully attempted this methodology recently as the detection tool for the first time in marine RAS and identified the diverse proteins of nitrifying bacterial consortia which are involved in transcription of nitrifying/denitrifying genes, pH regulation and ATP transport of nitrifiers/denitrifiers (Jose et al 2020). As Ruiz et al (2020) suggested, the technologies should be improvised to develop molecules which can induce desired nitrifying-denitrifying genes necessary for the activation of bioreactors.…”

Section: Current Application Status and Prospects Of Nitrification An...mentioning

confidence: 99%

“…This indicates the inevitability of the autotrophic nitrifiers to perform to the maximum so as to obtain sufficient energy for survival, which invariably ensures the sustainability and efficiency of chemolithoautotrophic nitrification in the ecosystem. Aquaculture is very much benefited of this phenomenon and the technological innovations introduced through nitrifying bioreactors (Kumar et al 2009a,b) and the required nitrifying bacterial consortia (Achuthan et al 2006) help establish instantaneous nitrification and denitrification (Preena et al 2017(Preena et al , 2018(Preena et al , 2019Jose et al 2020). These developments shall yield rich dividend to sustainable aquaculture production systems in fresh, brackish and marine environments.…”

Section: Current Application Status and Prospects Of Nitrification An...mentioning

confidence: 99%

“…2011; Jose et al . 2020). Very recently, simultaneous partial nitrification, anammox and denitrification (SNAD) bioreactor was successfully demonstrated for the first time in RAS with high dissolved oxygen conditions and without any additional organic carbon supply (Lu et al .…”

Section: Current Application Status and Prospects Of Nitrification An...mentioning

confidence: 99%

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Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Preena

Kumar

Singh

2021

Reviews in Aquaculture

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One of the major challenges in any sustainable aquaculture production systems is the accumulation of nitrogenous waste such as ammonia and its biological nitrification products viz nitrite and nitrate. Considering the bio-security issues, amelioration of these wastes without water exchange can be accomplished only by way of establishing in situ nitrification and denitrification through biofilters/ bioreactors activated with nitrifying/denitrifying bioaugmentors. In such systems, coexistence of aerobic denitrifiers, anaerobic ammonia oxidizers (Anammox) and complete ammonia oxidizers (Comammox) together with the autotrophic nitrifiers enhance the coupled nitrification-denitrification. This promotes total nitrogen removal without external carbon supplements or additional anerobic compartment in the system. Various recirculating aquaculture systems (RAS) comprise diverse nitrifying community in biofilters/bioreactors thereby imparting distinctive nitrogen conversion in the system. Meanwhile, the structure and population dynamics of the nitrifying/denitrifying consortia are influenced by the environmental factors forming the decisive factors of the success of the processes. Accordingly, understanding the complexity of nitrifying/denitrifying community composition turns out to be a requirement to facilitate its improvised performance. In this context, the review addresses different biological nitrogen removal systems, significance of nitrification-denitrification in RAS, genetic diversity of the key players in RAS, methods of analysis of their community structure, current application and prospects of the processes in RAS.

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Section: Current Application Status and Prospects Of Nitrification An...mentioning

confidence: 99%

Section: Current Application Status and Prospects Of Nitrification An...mentioning

confidence: 99%

Section: Current Application Status and Prospects Of Nitrification An...mentioning

confidence: 99%

Section: Current Application Status and Prospects Of Nitrification An...mentioning

confidence: 99%

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Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Preena

Kumar

Singh

2021

Reviews in Aquaculture

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“…Both qPCR and 16S-rRNA gene amplicon sequencing are well-established methods to determine the composition of microbial communities of (de)nitrification bioreactors [25,[27][28][29][30][31]. A metaproteomic approach is more often used to characterize the functionality and to identify novel functional proteins and their respective metabolic pathways in those bioreactors [30][31][32]. Moreover, the technique can also be used as a taxonomical identification tool [33].…”

Section: Introductionmentioning

confidence: 99%

Metaproteomics, Heterotrophic Growth, and Distribution of Nitrosomonas europaea and Nitrobacter winogradskyi after Long-Term Operation of an Autotrophic Nitrifying Biofilm Reactor

Mastroleo

Arnau

Verbeelen

et al. 2022

Applied Microbiology

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Bioregenerative life support systems (BLSS) are currently in development to tackle low recovery efficiencies, high energy demands, as well as food, water, and oxygen production challenges through the regeneration of nutrients from waste streams. The MELiSSA pilot plant has been developed as a testbed for regenerative life support system bioreactor operation and characterization. As nitrogen is a vital resource in such systems, we studied the functional composition of a new packed-bed nitrifying bioreactor inoculated with a co-culture of Nitrosomonas europaea (ATCC 25978) and Nitrobacter winogradskyi (ATCC 25391). After 840 days of autotrophic continuous cultivation, the packed-bed was sampled at five vertical positions, each with three horizontal positions, and the biomass at each position was characterized via qPCR, 16S amplicon sequencing, and liquid chromatography tandem mass spectrometry. The total number of cells within the different sections fluctuated around 8.95 ± 5.10 × 107 cells/mL of beads. Based on 16S amplicons and protein content, N. europaea and N. winogradskyi constituted overall 44.07 ± 11.75% and 57.53 ± 12.04% of the nitrifying bioreactor, respectively, indicating the presence of a heterotrophic population that, even after such a long operation time, did not affect the nitrification function of the bioreactor. In addition, DNA-based abundance estimates showed that N. europaea was slightly more abundant than N. winogradskyi, whereas protein-based abundance estimates indicated a much higher abundance of N. europaea. This highlights that single-method approaches need to be carefully interpreted in terms of overall cell abundance and metabolic activity.

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Microbiomes in the context of developing sustainable intensified aquaculture

et al. 2023

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With an ever-growing human population, the need for sustainable production of nutritional food sources has never been greater. Aquaculture is a key industry engaged in active development to increase production in line with this need while remaining sustainable in terms of environmental impact and promoting good welfare and health in farmed species. Microbiomes fundamentally underpin animal health, being a key part of their digestive, metabolic and defense systems, in the latter case protecting against opportunistic pathogens in the environment. The potential to manipulate the microbiome to the advantage of enhancing health, welfare and production is an intriguing prospect that has gained considerable traction in recent years. In this review we first set out what is known about the role of the microbiome in aquaculture production systems across the phylogenetic spectrum of cultured animals, from invertebrates to finfish. With a view to reducing environmental footprint and tightening biological and physical control, investment in “closed” aquaculture systems is on the rise, but little is known about how the microbial systems of these closed systems affect the health of cultured organisms. Through comparisons of the microbiomes and their dynamics across phylogenetically distinct animals and different aquaculture systems, we focus on microbial communities in terms of their functionality in order to identify what features within these microbiomes need to be harnessed for optimizing healthy intensified production in support of a sustainable future for aquaculture.

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Metaproteomic insights into ammonia oxidising bacterial consortium developed for bioaugmenting nitrification in aquaculture systems

Cited by 3 publications

References 27 publications

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Metaproteomics, Heterotrophic Growth, and Distribution of Nitrosomonas europaea and Nitrobacter winogradskyi after Long-Term Operation of an Autotrophic Nitrifying Biofilm Reactor

Microbiomes in the context of developing sustainable intensified aquaculture

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