Metaproteomics reveals major microbial players and their biodegradation functions in a large‐scale aerobic composting plant

Abstract

Composting is an appropriate management alternative for municipal solid waste; however, our knowledge about the microbial regulation of this process is still scare. We employed metaproteomics to elucidate the main biodegradation pathways in municipal solid waste composting system across the main phases in a large-scale composting plant. The investigation of microbial succession revealed that Bacillales, Actinobacteria and Saccharomyces increased significantly with respect to abundance in composting process. The key microbiologic population for cellulose degradation in different composting stages was different. Fungi were found to be the main producers of cellulase in earlier phase. However, the cellulolytic fungal communities were gradually replaced by a purely bacterial one in active phase, which did not support the concept that the thermophilic fungi are active through the thermophilic phase. The effective decomposition of cellulose required the synergy between bacteria and fungi in the curing phase.

Development of an Analytical Method for the Metaproteomic Investigation of Bioaerosol from Work Environments

“…In fact, culture dependent methods are limited due to the difficulty of accessing every genotype from the fungal or bacterial community and the low culturability of bacteria and fungi occurring in bioaerosol. Our data also agreed with a previous metaproteomic work carried out directly on urban solid waste in a large‐scale aerobic CP; in the paper, the authors highlighted that some bacteria, such as Bacillales , Actinobacteria , and Saccharomyces increased significantly compared to their abundance in the composting process . Our work represents a step forward in this field since the information related to bioaerosol is more important compared to information on urban solid waste, as bioaerosol can travel up to 1 km from the point of emission, leading to risks of infection not only for workers in the composting facility but also for nearby residents.…”

Microbiota and Antibiotic Resistome of Lettuce Leaves and Radishes Grown in Soils Receiving Manure-Based Amendments Derived From Antibiotic-Treated Cows

“…While the radish samples had a significant increase in Psuedomonadaceae when grown in raw-manure amended soils, they carried roughly 40 times more OTUs identified in the order Methylophilales when grown in compost-amended soil. Methylophilales have been identified as important microbes in large-scale aerobic composting processes due to biodegradation properties of the bacteria (Liu et al, 2015). Thus, the results are consistent with the compost having influenced the microbiota of the vegetables.…”

Metaproteomics analysis of the functional insights into microbial communities of combined hydrogen and methane production by anaerobic fermentation from reed straw

“…Proteins were extracted from 50 mL samples using the method published by Benndorf et al . [ 18 ] and subsequently separated by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE) as described previously [ 19 ]. The protein pellet was dissolved in 20 μL sample buffer for SDS-PAGE and incubated for 20 min at 100°C.…”