Metarhizium anisopliae E6 secretome reveals molecular players in host specificity and toxicity linked to cattle tick infection

Oliveira, Laura Rascovetzki Saciloto de; Broetto, Leonardo; Alves, Camila Innocente; Rosa, Rafael Lopes da; Alves, Yohana Porto Calegari; Silva, Rodrigo Campos da; Oliveira, Markus Berger; Macêdo, Alexandre José; Dalberto, Pedro Ferrari; Bizarro, Cristiano Valim; Guimarães, Jorge A.; Santi, Lucélia; Beys‐da‐Silva, Walter O.

doi:10.1016/j.funbio.2023.06.006

Cited by 2 publications

(1 citation statement)

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“…The results obtained in this work showed that M. robertsii Mt015 isolate secreted the highest levels of protease, followed by M. brunneum and M. anisopliae isolates. A common feature of these three species is their generalist nature, being able to parasitize over seven arthropod orders [ 40 ]. According to previous studies, protease expression levels of Metarhizium change with hosts, suggesting a mechanism of interaction of fungal pathogens and their arthropod hosts through differential selective pressures on virulence factors [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Fungal Lytic Enzymatic Extracts Produced Under Submerged Fermentation as Enhancers of Entomopathogens’ Biological Activity

Mejía,

Bautista,

García

et al. 2024

Curr Microbiol

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The application of enzymes in agricultural fields has been little explored. One potential application of fungal lytic enzymes (chitinases, lipases, and proteases) is as an additive to current biopesticides to increase their efficacy and reduce the time of mortality. For this, a screening of lytic overproducer fungi under submerged fermentation with a chemical-defined medium was performed. Then, the enzymatic crude extract (ECE) was concentrated and partially characterized. This characterization consisted of measuring the enzymatic activity (lipase, protease and, chitinase) and determining the enzyme stability after storage at temperatures of − 80, − 20 and, 4 °C. And lastly, the application of these concentrated enzymatic crude extracts (C-ECE) as an enhancer of spores-based fungal biopesticide was proven. Beauveria were not as good producers of lytic enzymes as the strains from Trichoderma and Metarhizium. The isolate M. robertsii Mt015 was selected for the co-production of chitinases and proteases; and the isolate T. harzianum Th180 for co-production of chitinases, lipases, and proteases. The C-ECE of Mt015 had a protease activity of 18.6 ± 1.1 U ml−1, chitinase activity of 0.28 ± 0.01 U ml−1, and no lipase activity. Meanwhile, the C-ECE of Th180 reached a chitinase activity of 0.75 U ml−1, lipase activity of 0.32 U ml−1, and protease activity of 0.24 U ml−1. Finally, an enhancing effect of the enzymatic extracts of M. robertsii (66.7%) and T. harzianum (43.5%) on the efficacy of B. bassiana Bv064 against Diatraea saccharalis larvae was observed. This work demonstrates the non-species-specific enhancing effect of enzymatic extracts on the insecticidal activity of conidial-based biopesticides, which constitutes a contribution to the improvement of biological control agents’ performance.

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Section: Discussionmentioning

confidence: 99%