Metasequencing of V3-V4 Variable Regions of 16S rRNA Gene in Opportunistic Microbiota and Gut Biocenosis in Obese Adolescents

Grigorova, E. V.; Belkova, Natalia; Nemchenko, U. M.; Klimenko, E. S.; Погодина, Анна; Romanitsa, A. I.; Новикова, Е. А.; Rychkova, Lyubov

doi:10.1007/s10517-021-05060-3

Cited by 10 publications

(7 citation statements)

References 12 publications

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“…Proteobacteria mainly include Alphaproteobacteria , Betaproteobacteria , Gammaproteobacteria , delta/epsilon subdivisions, and Zetaproteobacteria , among which Gammaproteobacteria participates in the metabolism of choline, and it has a high abundance in overweight/obese children with non-alcoholic fatty liver disease (NAFLD) ( 68 , 69 ). Enterobacteriaceae , a member of Gammaproteobacteria , is commonly observed in overweight adolescents ( 43 ). The abundance of Escherichia coli is also significantly higher in obese children ( 44 ).…”

Section: The Relationship Between Pediatric Overweight/obesity and Gu...mentioning

confidence: 99%

Roles of gut microbiota and metabolites in overweight and obesity of children

Zhang

Dang

2022

Front. Endocrinol.

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The prevalence of overweight and obesity in children and adolescents is an increasing public health problem. Pediatric overweight and obesity result from multiple factors, including genetic background, diet, and lifestyle. In addition, the gut microbiota and their metabolites play crucial roles in the progression of overweight and obesity of children. Therefore, we reviewed the roles of gut microbiota in overweight/obese children. The relationship between pediatric overweight/obesity and gut metabolites, such as short-chain fatty acids, medium-chain fatty acids, amino acids, amines, and bile acids, are also summarized. Targeting gut microbiota and metabolites might be a promising strategy for interventions aimed at reducing pediatric overweight/obesity.

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Section: The Relationship Between Pediatric Overweight/obesity and Gu...mentioning

confidence: 99%

Roles of gut microbiota and metabolites in overweight and obesity of children

Zhang

Dang

2022

Front. Endocrinol.

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“…Следует отметить, что уменьшение содержания микроорганизмов, свойственных нормальной биоте толстой кишки (бифидо-и лактобактерий), было выявлено не только у подростков с ФРК, но и в группе контроля, однако КП с ИС и УПМ сем. Enterobacteriaceae в диагностически значимой концентрации регистрировали только в основной группе, что говорит о более глубоких дисбиотических нарушениях в биоценозе толстой кишки у подростков с ожирением [13].…”

Section: Discussionunclassified

“…Анализ доступной литературы предоставляет весомые доказательства связи ожирения с диареей во взрослой когорте, тогда как большинство педиатрических исследований дают противоположные результаты, свидетельствуя о связи ожирения с запорами. На каком этапе и в силу каких причин происходит смена направления ассоциации между ожирением и паттерном функционирования толстой кишки остаётся неясным, но изменённая микробиота и связанные с ней воспаление и повышенная проницаемость кишечника могут играть ключевую роль [7,13,14].…”

Section: педиатрия Pediatricsunclassified

“…Исследование 20 образцов фекалий проводили стандартными бактериологическими методами (путём посева на специальные питательные среды) [13,17]; учёт количественного и качественного состава микробиоты кишечника -согласно отраслевому стандарту «Дисбактериоз кишечника. Протокол ведения больных» (ОСТ 91500.11.0004-2003).…”

Section: методы исследованияunclassified

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Associations of clinical features of functional bowel disorders with gut microbiota characteristics in adolescents: A pilot study

Romanitsa

Nemchenko

Pogodina

et al. 2021

Acta biomedica scientifica

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Background. Obesity is a multifactorial disease that is one of the important public health problems in the 21st century. Functional bowel disorders in obese children and adolescents are one of the most common pathologies of the digestive tract. The aim: to characterize the bacteriological composition of the colon microbiota in obese adolescents and identify its associations with the clinical manifestations of functional bowel disorders.Materials and methods. The study included 20 adolescents aged 11–17 years. Stool consistency was assessed using the Bristol Stool Scale. The study of biological material was carried out using standard bacteriological methods. To assess the strength of the relationship between the nominal variables, Cramer’s V test was used.Results. It has been shown that in adolescents with obesity and functional bowel disorders, there is a decrease in the number of representatives of bifidoflora, an increase in the microbial density of Escherichia coli with altered enzymatic properties and an excessive growth of potentially pathogenic enterobacteria. In obese adolescents with functional bowel disorders, abdominal pain associated with the act of defecation is associated with the presence of enterobacteria and E. coli with altered enzymatic properties. Rare bowel movements are associated with an increased abundance of Enterococcus spp. and the presence of opportunistic Enterobacteriaceae microorganisms. Frequent bowel movements are associated with the presence of E. coli with altered enzymatic properties and Clostridium spp. In the presence of loose stool, Clostridium spp. is present; unstable stool (alternation of hard and liquid stools in one patient) is associated with a decrease in the number of lactobacilli and E. coli with normal enzymatic properties, the appearance of E. coli with altered enzymatic properties, opportunistic microorganisms of Enterobacteriaceae family and Clostridium spp. Conclusion. The relationship between the clinical symptoms of functional bowel disorders and changes in the composition of the gut microbiota in obese adolescents may be a justification for conducting in-depth studies aimed at finding early predictors of functional bowel diseases associated with obesity in the adult cohort.

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“…However, Proteobacteria are also relatively abundant among malnourished children, with decreasing levels inversely to physical exercise ( 96 ). Among them, Gammaproteobacteria , especially Enterobacteriaceae to which Escherichia coli belong, participate in the metabolism of choline and they are particularly expressed in overweight/obese children with NAFLD ( 97 – 99 ). In accordance, the abundance of Escherichia coli is also significantly higher in obese children ( 100 ).…”

Section: Bas and Obesity: Current Knowledgementioning

confidence: 99%

Role of bile acids in overweight and obese children and adolescents

et al. 2022

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Bile acids (BAs) are amphipathic molecules synthetized in the liver. They are primarily involved in the digestion of nutrients. Apart from their role in dietary lipid absorption, BAs have progressively emerged as key regulators of systemic metabolism and inflammation. In the last decade, it became evident that BAs are particularly important for the regulation of glucose, lipid, and energy metabolism. Indeed, the interest in role of BA in metabolism homeostasis is further increased due to the global public health increase in obesity and related complications and a large number of research postulating that there is a close mutual relationship between BA and metabolic disorders. This strong relationship seems to derive from the role of BAs as signaling molecules involved in the regulation of a wide spectrum of metabolic pathways. These actions are mediated by different receptors, particularly nuclear farnesoid X receptor (FXR) and Takeda G protein coupled receptor 5 (TGR5), which are probably the major effectors of BA actions. These receptors activate transcriptional networks and signaling cascades controlling the expression and activity of genes involved in BA, lipid and carbohydrate metabolism, energy expenditure, and inflammation. The large correlation between BAs and metabolic disorders offers the possibility that modulation of BAs could be used as a therapeutic approach for the treatment of metabolic diseases, including obesity itself. The aim of this review is to describe the main physiological and metabolic actions of BA, focusing on its signaling pathways, which are important in the regulation of metabolism and might provide new BA -based treatments for metabolic diseases.

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Metasequencing of V3-V4 Variable Regions of 16S rRNA Gene in Opportunistic Microbiota and Gut Biocenosis in Obese Adolescents

Cited by 10 publications

References 12 publications

Roles of gut microbiota and metabolites in overweight and obesity of children

Roles of gut microbiota and metabolites in overweight and obesity of children

Associations of clinical features of functional bowel disorders with gut microbiota characteristics in adolescents: A pilot study

Role of bile acids in overweight and obese children and adolescents

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