Metasilicate-based alkaline mineral water confers diarrhea resistance in maternally separated piglets via the microbiota-gut interaction

Chen, Jian; Xu, Xiaoshu; Kang, Jian-Xun; Zhao, Bi-Chen; Xu, Yaru; Li, Jinlong

doi:10.1016/j.phrs.2022.106580

Cited by 20 publications

(19 citation statements)

References 67 publications

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“…Three complete heart tissues were collected from each group for histopathological observation. The procedures are consistent with the previous study ( Chen et al, 2023a ). Briefly, tissues were embedded in paraffin, cut to a thickness of 5 μm with a Leica automatic slicer (HistoCore AUTOCUT, Leica, Germany), and then placed at 37°C for drying.…”

Section: Methodssupporting

confidence: 88%

Inhibition of mtDNA-PRRs pathway-mediated sterile inflammation by astragalus polysaccharide protects against transport stress-induced cardiac injury in chicks

Chen,

Xu,

et al. 2024

Poultry Science

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Section: Methodssupporting

confidence: 88%

Inhibition of mtDNA-PRRs pathway-mediated sterile inflammation by astragalus polysaccharide protects against transport stress-induced cardiac injury in chicks

Chen,

Xu,

et al. 2024

Poultry Science

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“…On the basis of a previous method, the brain tissues were embedded in paraffin and used for HE staining, Nissl staining, and Bielschowsky silver staining. − The slides were scanned using a microscope Panoramic MIDI (3DHISTECH Ltd., Budapest, Hungary). The numbers of total cells, neurons, and nerve fibers were quantified using ImageJ software according to a recent method …”

Section: Methodsmentioning

confidence: 99%

Phthalates Induce Neurotoxicity by Disrupting the Mfn2-PERK Axis-Mediated Endoplasmic Reticulum–Mitochondria Interaction

Zhao,

Chang,

Ren

et al. 2024

J. Agric. Food Chem.

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phthalate (DEHP), as the most common phthalate, has been extensively used as a plasticizer to improve the plasticity of agricultural products, which pose severe harm to human health. Mitochondrial dynamics and endoplasmic reticulum (ER) homeostasis are indispensable for maintaining mitochondria-associated ER membrane (MAM) integrity. In this study, we aimed to explore the effect of DEHP on the nervous system and its association with the ER−mitochondria interaction. Here, we showed that DEHP caused morphological changes, motor deficits, cognitive impairments, and blood−brain barrier disruption in the brain. DEHP triggered ER stress, which is mainly mediated by protein kinase R-like endoplasmic reticulum kinase (PERK) signaling. Moreover, DEHP-induced mitofusin-2 (Mfn2) downregulation results in imbalance of the mitochondrial dynamics. Interestingly, DEHP exposure impaired MAMs by inhibiting the Mfn2-PERK interaction. Above all, this study elucidates the disruption of the Mfn2-PERK axis-mediated ER−mitochondria interaction as a phthalate-induced neurotoxicity that could be potentially developed as a novel therapy for neurological diseases.

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“…The model of MS piglets was used according to previous study [ 5 ]. A total of 240 piglets from 40 different sows (3 or 4 parity, 6 piglets per litter) were selected.…”

Section: Methodsmentioning

confidence: 99%

“…Then, 12 replicates were randomly divided into two drinking water treatment groups (six replicate pens per group and 20 piglets per pen): (1) Control group, consumed basal water (pH = 7.0); and (2) SMP group, consumed silicon-rich AMW (basal water + 500 mg/L SMP). Various studies clearly demonstrated that, compared with un-weaned piglets, MS/weaning stress causes severe IEI in piglets, as manifested by intestinal villus atrophy and functional cell loss [ 5 , 21 ]; thus, no stress-free control group was set in this study. All piglets were administered an identical feed diet (NRC, 2012) and had unlimited access to food and water in this study.…”

Section: Methodsmentioning

confidence: 99%

Role of the GLP2–Wnt1 axis in silicon-rich alkaline mineral water maintaining intestinal epithelium regeneration in piglets under early-life stress

Chen,

Dai,

Zhao

et al. 2024

Cell. Mol. Life Sci.

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Stress-induced intestinal epithelial injury (IEI) and a delay in repair in infancy are predisposing factors for refractory gut diseases in adulthood, such as irritable bowel syndrome (IBS). Hence, it is necessary to develop appropriate mitigation methods for mammals when experiencing early-life stress (ELS). Weaning, as we all know, is a vital procedure that all mammalian newborns, including humans, must go through. Maternal separation (MS) stress in infancy (regarded as weaning stress in animal science) is a commonly used ELS paradigm. Drinking silicon-rich alkaline mineral water (AMW) has a therapeutic effect on enteric disease, but the specific mechanisms involved have not been reported. Herein, we discover the molecular mechanism by which silicon-rich AMW repairs ELS-induced IEI by maintaining intestinal stem cell (ISC) proliferation and differentiation through the glucagon-like peptide (GLP)2–Wnt1 axis. Mechanistic study showed that silicon-rich AMW activates GLP2-dependent Wnt1/β-catenin pathway, and drives ISC proliferation and differentiation by stimulating Lgr5+ ISC cell cycle passage through the G1–S-phase checkpoint, thereby maintaining intestinal epithelial regeneration and IEI repair. Using GLP2 antagonists (GLP23−33) and small interfering RNA (SiWnt1) in vitro, we found that the GLP2–Wnt1 axis is the target of silicon-rich AMW to promote intestinal epithelium regeneration. Therefore, silicon-rich AMW maintains intestinal epithelium regeneration through the GLP2–Wnt1 axis in piglets under ELS. Our research contributes to understanding the mechanism of silicon-rich AMW promoting gut epithelial regeneration and provides a new strategy for the alleviation of ELS-induced IEI.

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Metasilicate-based alkaline mineral water confers diarrhea resistance in maternally separated piglets via the microbiota-gut interaction

Cited by 20 publications

References 67 publications

Inhibition of mtDNA-PRRs pathway-mediated sterile inflammation by astragalus polysaccharide protects against transport stress-induced cardiac injury in chicks

Inhibition of mtDNA-PRRs pathway-mediated sterile inflammation by astragalus polysaccharide protects against transport stress-induced cardiac injury in chicks

Phthalates Induce Neurotoxicity by Disrupting the Mfn2-PERK Axis-Mediated Endoplasmic Reticulum–Mitochondria Interaction

Role of the GLP2–Wnt1 axis in silicon-rich alkaline mineral water maintaining intestinal epithelium regeneration in piglets under early-life stress

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