Metastasis of Lewis Lung Carcinoma (LL) Regrowing after Cytotoxic Treatments

Stephens, T C; Adams, K; Peacock, John

doi:10.1007/978-94-009-8925-2_80

Cited by 4 publications

(5 citation statements)

References 4 publications

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“…In these mutants DHL-DH activities were significantly lower than in the wild-type. Partial sequence analysis of phbL revealed strong homology to the DHL-DH gene In E. colt Ipd is located downstream of the ace operon that encodes the pyruvate dehydrogenase (aceE) and dihydrolipoamide acetyltransferase (aceF) components of the pyruvate dehydrogenase complex, but is expressed from its own promoter [25]. This, and excretion of pyruvate might indicate that the reaction catalyzed by the pyru-vate dehydrogenase complex is affected in subclass B mutants and that the provision of acetyl-CoA for PHB biosynthesis is decrea~d.…”

Section: S Discussionmentioning

confidence: 99%

“…The Sail subfragment conferring kanamycin resistance from one hybrid cosmid was ligated to pBluescript SK(-). The amino acid sequence deduced from the nucleotide sequence of this open reading frame exhibited in an overlap of 98 amino acids 60% identity to a region close to the C-terminus of the lipoamide dehydrogenase of E. coil [25] (Fig. From this sequence a second oligonucleotide was derived and used as primer in a sequencing reaction with pSK6300-DNA as template• A nucleotide sequence of 298 bp was obtained, which revealed one open reading frame and which most probably repre~nted a coding DNA region according to Bibb et al [24] (Fig.…”

Section: Partial Nucleotide Sequence Of the Phbl Locusmentioning

confidence: 99%

“…With all plasmids and with genomic DNA of strain HI6 a 63(X)-bp fragment gave a strong hybridization signal, in contrast, with genomic DNA of strain H!482 a fragment of approximately 14-kbp gave a signal. 3), indicating that the formation of a second lipoamide dehydrogenase, which (aceE) and dihydrolipoamide acetyltransferase (aceF) components of the pyruvate dehydrogenase complex, but is expressed from its own promoter [25]. 3).…”

Section: Hybrmization Ea'perimotts Nqth a Lipoamide Dehydrogenase Spementioning

confidence: 99%

“…The molar G + C content of the sequence was 66%; for the first, second and third codon positions the G + C contents were 64, 51 and 83%, respectively. The amino acid sequence deduced from the nucleotide sequence of this open reading frame exhibited in an overlap of 98 amino acids 60% identity to a region close to the C-terminus of the lipoamide dehydrogenase of E. coil [25] (Fig. 2).…”

Section: Partial Nucleotide Sequence Of the Phbl Locusmentioning

confidence: 99%

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Identification of a lipoamide dehydrogenase gene as second locus affected in poly(3-hydroxybutyric acid)-leaky mutants of Alcaligenes eutrophus

Pries

Hein

Steinbüchel

1992

FEMS Microbiology Letters

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From a genomic library of Alcaligenes eutrophus strain H16 in the broad-host range cosmid pVK100 a 6300-bp EcoRI-fragment was cloned, which restored the wild-type phenotype in transposon-induced poly(3-hydroxybutyric acid)-leaky mutants, derived from A. eutrophus. Nucleotide sequence analysis of the region adjacent to the transposon insertion revealed an open reading frame which complied with all criteria for a coding region. This region was referred to as phbL, and the deduced amino acid sequence from this part of phbL showed 60% amino acid identity in an overlap of 98 residues to the lipoamide dehydrogenase gene (lpd) of Escherichia coli. In addition, the 6300-bp EcoRI-fragment conferred expression of lipoamide dehydrogenase activity to E. coli.

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Section: S Discussionmentioning

confidence: 99%

Section: Partial Nucleotide Sequence Of the Phbl Locusmentioning

confidence: 99%

Section: Hybrmization Ea'perimotts Nqth a Lipoamide Dehydrogenase Spementioning

confidence: 99%

Section: Partial Nucleotide Sequence Of the Phbl Locusmentioning

confidence: 99%

See 2 more Smart Citations

Identification of a lipoamide dehydrogenase gene as second locus affected in poly(3-hydroxybutyric acid)-leaky mutants of Alcaligenes eutrophus

Pries

Hein

Steinbüchel

1992

FEMS Microbiology Letters

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“…It is likely that the different results obtained in response to cyclophosphamide by Van Putten's group [7] and Poupon's group [8] relate to the nature of the tumour system used, since sub-curative doses of this drug caused another turnout, the Lewis lung carcinoma, to metastasize at smaller sizes after re-growth than when tumours were allowed to develop untreated [10].…”

Section: Drug Induced Enhancement Of Metastasis In Animal Modelsmentioning

confidence: 99%

Can cancer chemotherapy enhance the malignant behaviour of tumours?

McMillan¹,

Hart²

1987

Cancer Metast Rev

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Cancer chemotherapy is currently undergoing an intensive reappraisal because of its unimpressive performance against the major common cancers. There are a number of possible reasons for this lack of success; one considered here is that under some circumstances anti-neoplastic drug treatment actually increases the malignant behaviour of tumours. Support for this idea comes mainly from experimental studies in which drug treatments increased metastatic spread. Investigation of this phenomenon shows that drug induced modifications of the host, including immunosuppression and vascular damage, can indeed facilitate metastasis. In addition, new data are presented demonstrating that the direct action of drugs on the tumour cells themselves can have similar enhancing effects. The possible mechanisms underlying such direct effects are discussed and the ability of anti-cancer drugs to cause genetic mutations, amplify genes, and alter gene expression are considered. While the nature and extent of this facilitation of tumour malignancy is not fully understood, it is suggested that this possibility should be considered in the design of treatment protocols.

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