Metastatic behaviour of human colon carcinoma in nude mice

Measurement of the survival of human tumor cells after implantation in athymic nude mice

“…Furthermore, this situation shows probably closer resemblance to the frequently applied procedure of tumor fragment implantation. The survival rate of PC-3 cells implanted into nude mice was low in comparison with that reported for other tumor systems, such as L1210 cells in C3D2F1 J mice (Hofer et al, 1969) and Co115 colon carcinoma cells (Sordat et al, 1982) or BRO melanoma cells (Lockshin et al, 1984a,b) in nude mice. Such differences could be partly explained by variations in the susceptibility of tumor cells to immunological and other factors and may ultimately reflect the well-known differences in transplantability between the various tumor types.…”

“…Other investigators who have used radioactivelylabelled tumor cells to study tumor-cell distribution, cell survival or related subjects have employed other radioactive tracers such as '251-IUdR (iododeoxyuridine) (Hofer et al, 1969;Sordat et al, 1982;Lockshin et al, 1984b) or "'Indium oxide (Lockshin et al, 1984~). However, the most important difference between the method described here and those reported earlier is the attachment of tumor cells to Millipore filter disks in contrast to the use of cell suspensions.…”

“…It has been reported that the site of implantation, i.p. versus S.C. (Hofer et al, 1969;Lockshin et al, 1984b), and the number of cells inoculated (Sordat et a/., 1982) also have a great impact on tumor cell survival.…”

Human Co115 colon carcinoma cells potentiate the degradation of laminin mediated by tissue‐type plasminogen activator

“…In contrast, the role of t-PA in invasive processes is still not evident, perhaps because most tumor cells produce u-PA instead of t-PA with some exceptions such as melanoma, neuroblastoma, and certain leukemic cells (Bizik et al, 1990;Neuman et al, 1989;Quax et al, 1991b;Wilson et al, 1983). The human colon carcinoma co115 cell line, which produces only t-PA and was established in our laboratory as a tumorigenic and metastatic cell line in nude mice (Sordat and Bogenmann, 1980), provides as a unique opportunity to investigate the mechanism of ECM degradation by t-PA in the absence of interfering effects of u-PA or PAIs. A more detailed analysis of (30115 cells confirmed that they produce only t-PA, as examined by zymographic analysis and antigenic determinations of PAS and PAIs.…”

“…We established previously a human colon carcinoma cell line named co115 (Carrel et al, 1976) which produces in culture only t-PA, but no u-PA (Cajot et al, 19861, and which is able to degrade the R22 ECM in vitro (Cajot et al, 1990). These cells were shown to be highly invasive and metastatic in vivo in nude mice (Sordat and Bogenmann, 1980). Recent studies showed that t-PA is able to 0 1994 WILEY-LISS, INC.…”