Metastatic potential of human colorectal carcinoma SW1222 cells transfected with cDNA encoding carcinoembryonic antigen

Abstract

In order to examine a role of carcinoembryonic antigen (CEA) in metastasis, cDNA encoding CEA was introduced into a clone of human colorectal carcinoma SW1222 cells. Western blot analysis revealed that all transfectants express CEA of 180 kDa while the parent clone does not. In the transfectants, the level of CEA expression in clone 3 was higher than that of clone 1. Clone 3 formed aggregates rapidly after suspended by trypsinization while clone 1 did not. In experimental metastasis assay where tumor cells were injected intrasplenically, clone 3 exhibited a higher liver-metastatic activity than clone 1. Fab fragment of anti-CEA antibody significantly inhibited both the cell aggregation and the liver metastases caused by clone 3. These findings suggested that CEA expressed on the cell surface may play an important role in hepatic metastasis from colorectal carcinoma, possibly through its cell adhesion activity.

Carcinoembryonic Antigen and CD44 Variant Isoforms Cooperate to Mediate Colon Carcinoma Cell Adhesion to E- and L-selectin in Shear Flow

“…It is expressed in a number of tumors of epithelial origin, including colorectal carcinoma, lung adenocarcinoma, and mucinous ovarian carcinoma (30). CEA has been reported to promote the metastatic potential of colon cancer cells (31,32). The capacity of different colorectal cell lines to grow in nude mouse spleen and liver models correlates positively with CEA production (33).…”

CEACAM5 is correlated with Angio/Lymphangiogenesis of Prostatic Lesions

“…B In PCa, CEACAM5 expression patterns were well correlated with tumor Gleason grading, and there was significant difference in expression patterns (p=0.010). According to the histological grading, poorly differentiated carcinomas showed more cytoplasmic staining [8], while the well differentiated displayed more membranous CEACAM5 staining (Figure 2A, B, C). The 9 cases with membranous staining have lower Gleason grading while the 24 cases with cytoplasmic staining have higher Gleason grading on average.…”

“…Indeed, the tumorigenic functions of CEACAM5 have been demonstrated in both 3D culture of colon carcinoma cell lines in vitro [4] and CEABAC transgenic mice in vivo [5,6]. However, a number of studies have indicated that CEACAM5 contributes to the tumor invasion and metastasis in human colorectal carcinoma [7,8]. Most of the current studies on CEACAM5 focus on gastrointestinal carcinoma.…”

Contribution of proteomics to understanding the role of tumor‐derived exosomes in cancer progression: State of the art and new perspectives

“…They found that among the 171 unique proteins identified in these exosomes, many were associated with cancer cell proliferation, invasion, and metastasis such as c-Met protein (hepatocyte growth factor receptor), amphiregulin (AREG), various ephrins (EFNB1, EFNB2), and Eph receptors (EPHA2-8, EPHB1-4), components involved in Wnt (␤-Catenin and TNIK) and Ras (CRK and GRB2) signaling, and several key components associated with colorectal cancer, including CEACAM1 and 5, FAT1, and CDH17 [74]. It is a well-known fact that CEACAM5 (CEA) is found to be overexpressed in over 90% of human gastrointestinal and pancreatic cancers and, in particular it is associated with metastatic potential in colon cancer [78,79] (Table 1).…”