Metastatic sublines of an SV40 large T antigen immortalized human prostate epithelial cell line

Bae, Victoria L.; Jackson‐Cook, Colleen; Maygarden, Susan J.; Plymate, S.R.; Chen, Juza; Ware, Joy L.

doi:10.1002/(sici)1097-0045(19980301)34:4<275::aid-pros5>3.0.co;2-g

Cited by 119 publications

(121 citation statements)

References 18 publications

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“…Even though these nonmalignant cells are not completely normal due to their immortalization, P69SV40T cells were considered as a good representation of normal prostate epithelial cells. The characterization of P69SV40T cell line was carried out by Bae et al (1994Bae et al ( , 1998. The MnSOD protein was measured with Western blot and the protein band corresponding to the MnSOD in PC-3 Wt cells was B50% of that of nonmalignant immortalized human prostate cells (P69SV40T) (Figure 1a).…”

Section: Stable Transfection Of Mnsodmentioning

confidence: 99%

Manganese superoxide dismutase overexpression inhibits the growth of androgen-independent prostate cancer cells

et al. 2004

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Section: Stable Transfection Of Mnsodmentioning

confidence: 99%

Manganese superoxide dismutase overexpression inhibits the growth of androgen-independent prostate cancer cells

et al. 2004

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“…The M12 line was derived from tumors developed in nude mice injected with p69SV40T cells; these are human prostate epithelial cells immortalized with SV40T antigen (Bae et al, 1998). M12 cells express stem cell antigen (PSCA) and are SOX9 in prostate cancer cells R Drivdahl et al cytokeratin positive (Bae et al, 1998); they also express PSA when expression of the AR is induced (see below).…”

Section: Cell Lines and Culturementioning

confidence: 99%

“…M12 cells express stem cell antigen (PSCA) and are SOX9 in prostate cancer cells R Drivdahl et al cytokeratin positive (Bae et al, 1998); they also express PSA when expression of the AR is induced (see below). Cells were maintained at 371C in RPMI 1640 supplemented with 10 ng/ml EGF, 0.1 mM dexamethasone, 5 mg/ml insulin, 5 mg/ml transferrin, 5 ng/ml selenium, and 50 mg/ml gentamicin (RPMI/ ITS), in a 95% air/5% CO 2 atmosphere.…”

Section: Cell Lines and Culturementioning

confidence: 99%

Suppression of growth and tumorigenicity in the prostate tumor cell line M12 by overexpression of the transcription factor SOX9

et al. 2004

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Overexpression of mac25 in the prostate cancer cell line M12 effects a dramatic reversal of the transformed phenotype. cDNA array analysis of RNA from cells overproducing the mac25 protein (M12/mac25) indicated upregulation of the sex determining transcription factor SOX9. In this study, we have confirmed increased expression of SOX9 in M12/mac25 cells and have further investigated the physiological effects of increased SOX9 production. Greatly increased levels of SOX9 RNA and mature protein were demonstrated in cells transfected with a SOX9 cDNA (M12/SOX9), and gel mobility shift assays confirmed binding of nuclear protein from these cells to an oligonucleotide containing the SOX9 consensus binding sequence. M12/SOX9 cells assumed the spindleshaped morphology characteristic of M12/mac25 cells, suggesting that SOX9 mediates some effects of mac25. Elevated expression of SOX9 resulted in a decreased rate of cellular proliferation, cell cycle arrest in G0/G1, and increased sensitivity to apoptosis. Tumor development in athymic nude mice was inhibited by 80%. Finally, prostate-specific antigen and the androgen receptor, two genes whose expression is characteristic of differentiated cells, were both upregulated in M12/SOX9 cells. These data indicate that SOX9 contributes to growth regulation by mac25 via inhibition of cell growth and promotion of differentiation.

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“…The derivation of the M12 cell line has been previously described (Bae et al, 1994(Bae et al, , 1995(Bae et al, , 1998Jackson-Cook et al, 1996). Briefly, human prostate epithelial cells were immortalized with SV40-T antigen to produce the poorly tumorigenic P69SV40 T (P69) cell line.…”

Section: Cell Culturementioning

confidence: 99%

Increased manganese superoxide dismutase (SOD-2) is part of the mechanism for prostate tumor suppression by Mac25/insulin-like growth factor binding-protein-related protein-1

et al. 2003

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Increased expression of mac25/insulin-like growth factor binding-protein related protein-1 (IGFBP-rP1) in human breast and prostate epithelial cell lines results in the suppression of tumor growth. CDNA expression array analysis revealed increased manganese superoxide dismutase (SOD-2) expression in the mac25/IGFBP-rP1-transfected M12 human prostate cancer cell line compared to M12 control cells. SOD-2 has been postulated to be a tumor suppressor. SOD-2 was also increased in LNCaP cells stably transfected with mac25/IGFBP-rP1, but not in mac25/IGFBP-rP1-transfected PC-3 cells. Mac25 LNCaP cells had a marked decrease in tumor growth in nude mice compared to controls, but there was no difference in tumor growth in mac25 PC-3 cells compared to control. Phosphorylated Erk and Akt were increased in the M12 and LNCaP transfected mac25/ IGFBP-rP1 cells but not PC-3 mac25. Inhibition of PI-3 kinase results in a marked decrease in viability of the M12-mac25 cells compared to M12 controls. Cells treated with H 2 O 2 result in an increase in phospho-ERK. Transfection of SOD-2 in M12 cells markedly decreased tumor growth, apoptosis, G1 delay in the cell cycle, and expression of senescence associated b-galactosidase. These results suggest that one of the downstream mediators of the senescence-associated tumor suppression effect of mac25/IGFBP-rP1 is SOD-2.

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Metastatic sublines of an SV40 large T antigen immortalized human prostate epithelial cell line

Cited by 119 publications

References 18 publications

Manganese superoxide dismutase overexpression inhibits the growth of androgen-independent prostate cancer cells

Manganese superoxide dismutase overexpression inhibits the growth of androgen-independent prostate cancer cells

Suppression of growth and tumorigenicity in the prostate tumor cell line M12 by overexpression of the transcription factor SOX9

Increased manganese superoxide dismutase (SOD-2) is part of the mechanism for prostate tumor suppression by Mac25/insulin-like growth factor binding-protein-related protein-1

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