METCAM/MUC18 is a novel tumor and metastasis suppressor for the human ovarian cancer SKOV3 cells

Wu, Guang-Jer; Zeng, Guofang

doi:10.1186/s12885-016-2181-9

Cited by 19 publications

(57 citation statements)

References 37 publications

(103 reference statements)

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“…Furthermore, the tumors induced by the METCAM clone 2D were confined to small regions, as shown in the results of H&E and IHC [42], whereas the tumors induced by the control (vector) clone 3D developed serious tumors, suggesting that tumors from the 2D clone appeared to be dormant; thus METCAM/MUC18 may function similarly to other tumor/metastasis suppressors in other tumor cells [46].…”

Section: Consistent With the Results Inmentioning

confidence: 93%

“…The control cells, which were transfected with the empty vector that did not contain the human METCAM/MUC18 cDNA, were also G418R and should not express METCAM/MUC18, similar to the parental SK-OV-3 cells. Figure 3 shows the expression of METCAM/MUC18 in the two typical G418R clones was higher than the empty-vector control clone derived from SK-OV-3 cell line [42]. The above G418R clones from the SK-OV-3 cell line are then used to study effects of over-expression of the METCAM/MUC18 gene on their in vitro cellular behaviors, such as cellular motility and invasiveness, and on in vivo tumorigenesis and metastasis in animal models.…”

Section: Metcam/muc18 Expression In G418r-clones Derived From the Sk-mentioning

confidence: 99%

“…Figure 5 (left panel), Figure 5 (right panel) shows that final tumor weights of the METCAM clone 2D were also lower than those of the control (vector) clone 3D at both sites, indicating that over-expression of METCAM/MUC18 decreased the final tumor weights of SK-OV-3 cells in nude mice. [42].…”

Section: Over-expression Of Metcam/muc18 Suppressed In Vivo Tumorigenmentioning

confidence: 99%

“…To scrutinize the notion that METCAM/MUC18 may play a positive role in the development of ovarian cancer [41], we used the above clones to perform studies in model animals [42]. We determined effects of METCAM/MUC18 over-expression on in vivo tumorigenicity of SKOV3 cells in female nude mice after SC injection at either dorsal (DSC) or ventral (VSC) side.…”

Section: Over-expression Of Metcam/muc18 Suppressed In Vivo Tumorigenmentioning

confidence: 99%

“…First, we re-evaluated the expression of METCAM/MUC18 in one immortalized normal ovarian epithelial cell line (IOSE) and five human ovarian cancer cell lines, BG-1, HEY, CAOV-3, SK-OV-3 and NIHOVCAR3 [42], as shown in the following Figure 2. HuMETCAM protein structure.…”

Section: Metcam/muc18 Is Expressed At a Lower Level In The Ovarian Camentioning

confidence: 99%

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METCAM/MUC18 plays a Novel Tumor and Metastasis Suppressor Role in the Progression of Human Ovarian Cancer Cells

Wu¹

2017

OGIJ

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METCAM/MUC18, an integral membrane cell adhesion molecule (CAM) in the Iglike gene super-family, is capable of performing typical functions of CAMs, such as mediating cell-cell and cell-extracellular interactions, crosstalk with intracellular signaling pathways, and modulating social behaviors of cells. The role of METCAM/ MUC18 in the progression of ovarian cancer cells has not been well studied. Previous studies showed that METCAM/MUC18 is expressed in normal ovarian epithelial cells, but expressed at higher levels in ovarian cancer tissues, suggesting that METCAM/ MUC18 may serve as a biomarker for the malignant progression of clinical ovarian cancers and it has been implicated for playing a positive role in the progression of the cancer. Recently we provided evidence from in vitro and in vivo studies to suggest that the above notion is a fortuitous correlation and that METCAM/MUC18 actually serves as a tumor and metastasis suppressor for the malignant progression of ovarian cancer cells, similar to its role in the malignant progression of one mouse melanoma cell line and nasopharyngeal carcinoma type I. Many possible mechanisms mediated by this CAM during early tumor development and metastasis are suggested. Furthermore, we suggest that METCAM/MUC18 may be used a therapeutic reagent to arrest the malignant progression of clinical ovarian cancer.

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Section: Consistent With the Results Inmentioning

confidence: 93%

Section: Metcam/muc18 Expression In G418r-clones Derived From the Sk-mentioning

confidence: 99%

Section: Over-expression Of Metcam/muc18 Suppressed In Vivo Tumorigenmentioning

confidence: 99%

Section: Over-expression Of Metcam/muc18 Suppressed In Vivo Tumorigenmentioning

confidence: 99%

Section: Metcam/muc18 Is Expressed At a Lower Level In The Ovarian Camentioning

confidence: 99%

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METCAM/MUC18 plays a Novel Tumor and Metastasis Suppressor Role in the Progression of Human Ovarian Cancer Cells

Wu¹

2017

OGIJ

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Enforced Expression of METCAM/MUC18 Decreases In Vitro Motility and Invasiveness and Tumorigenesis and In Vivo Tumorigenesis of Human Ovarian Cancer BG-1 Cells

2021

Advances in Experimental Medicine and Biology

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Ectopic expression of MCAM/MUC18 increases in vitro motility and invasiveness, but decreases in vivo tumorigenesis and metastasis of a mouse melanoma K1735-9 subline in a syngeneic mouse model

2016

Clin Exp Metastasis

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Ectopic expression of MCAM/MUC18, a cell adhesion molecule in the immunoglobulin-like gene superfamily, induces two moMCAM/MUC18-minus, non-metastatic mouse melanoma K1735 sublines, K3 (tumor/met) and K10 (tumor/met), to metastasize to lungs in a syngeneic C3H mouse model. In this report, we extended investigation of effects of moMCAM/MUC18 expression on tumorigenesis and metastasis in another lowly metastatic, however highly tumorigenic moMCAM/MUC18-minus mouse melanoma K1735 subline, K9 (tumor/met). We transfected this subline with the moMCAM/MUC18 cDNA, selected for G418-resistant clones with different expression levels of moMCAM/MUC18, and used them for testing effects of MCAM/MUC18 expression on in vitro growth rate, motility, and invasiveness, in vivo subcutaneous tumor growth, and pulmonary metastasis in syngeneic C3H brown mice. Similar to K3 and K10 cells, increased expression of MCAM/MUC18 in K9 cells did not significantly affect in vitro growth rate, but increased in vitro motility and invasiveness. Surprisingly, increased expression of MCAM/MUC18 in K9 cells decreased their induction of tumorigenesis and suppressed their establishment of pulmonary nodules in syngeneic C3H brown mice. We concluded that increased MCAM/MUC18 expression in K9 subline increased in vitro epithelial-to-mesenchymal transition; however, it suppressed in vivo tumorigenicity and metastasis. Thus MCAM/MUC18 acts as a tumor and metastasis suppressor for the K9 subline, different from its role in other K1735 sublines, K3 and K10. Different intrinsic co-factors in different K1735 sublines, which modulate the functions of MCAM/MUC18 in the cells that interact differently to the tumor microenvironment, may render sublines manifest differently in tumorigenicity and metastasis in vivo.

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METCAM/MUC18 is a novel tumor and metastasis suppressor for the human ovarian cancer SKOV3 cells

Cited by 19 publications

References 37 publications

METCAM/MUC18 plays a Novel Tumor and Metastasis Suppressor Role in the Progression of Human Ovarian Cancer Cells

METCAM/MUC18 plays a Novel Tumor and Metastasis Suppressor Role in the Progression of Human Ovarian Cancer Cells

Enforced Expression of METCAM/MUC18 Decreases In Vitro Motility and Invasiveness and Tumorigenesis and In Vivo Tumorigenesis of Human Ovarian Cancer BG-1 Cells

Ectopic expression of MCAM/MUC18 increases in vitro motility and invasiveness, but decreases in vivo tumorigenesis and metastasis of a mouse melanoma K1735-9 subline in a syngeneic mouse model

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