Metformin attenuates streptozotocin-induced diabetic nephropathy in rats through modulation of oxidative stress genes expression

Alhaider, Abdulqader A.; Korashy, Hesham M.; Sayed‐Ahmed, Mohamed M.; Mobark, Mohammed; Kfoury, Hala; Mansour, Mahmoud A.

doi:10.1016/j.cbi.2011.03.014

Cited by 157 publications

(116 citation statements)

References 49 publications

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“…STZ is widely employed to induce experimental diabetic nephropathy as it selectively destroys the islet of Langerhans (Alhaider et al, 2011). In the present study, induction of diabetic nephropathy by STZ was evidenced by elevated levels of urinary total protein, albumin, serum creatinine, BUN, and decreased creatinine clearance, which were taken as direct in vivo index for nephropathy in STZ diabetic rats (Siddiqui et al, 2010).…”

Section: Discussionmentioning

confidence: 72%

Flavonoid rich fraction ofPunica granatumimproves early diabetic nephropathy by ameliorating proteinuria and disturbed glucose homeostasis in experimental animals

Ankita

Deepti

Nilam

2014

Pharmaceutical Biology

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Context: Different parts of Punica granatum Linn. (Punicaceae) are traditionally used as renal protective agents in the Indian system of medicine. However, there is paucity of information regarding its role in diabetic nephropathy. Objective: The present study investigates the nephroprotective potential of flavonoid-rich fraction of P. granatum leaves in streptozotocin (STZ)-induced early diabetic nephropathy in experimental animals. Materials and methods: Experimental diabetic nephropathy was induced in Wistar rats by single intraperitonial injection of STZ (65 mg/kg) dissolved in ice cold citrophosphate buffer (pH 4.3). After induction rats were divided into five groups (6 normal; 24 diabetic) and administered with glibenclamide (5 mg/kg) and three dose levels of flavonoid-rich fraction of P. granatum leaves (PGFF), i.e. 50, 100, and 200 mg/kg body weight/day for 28 d. Fasting blood glucose, lipid profile, serum albumin, serum total protein, serum creatinine, blood urea nitrogen (BUN) glycosylated hemoglobin, and biomarkers of kidney oxidative stress were assessed at the end of the treatment period. Urine was analyzed for the measurement of total protein, albumin, and creatinine clearance. Kidney sections were subjected to histopathological study. Results: Daily oral administration of variable dose levels of PGFF for 28 d normalized various biochemical, metabolic, and histopathological changes in the diabetic rats. PGFF significantly (p50.01 and p50.05) improved the glycemic status and renal function in diabetic rats as compared with diabetic control rats. Discussion and conclusion: The results of our study thus prove the protective effect of PGFF in early diabetic nephropathy by ameliorating proteinuria and disturbed glucose homeostasis in experimental animals.

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Section: Discussionmentioning

confidence: 72%

Flavonoid rich fraction ofPunica granatumimproves early diabetic nephropathy by ameliorating proteinuria and disturbed glucose homeostasis in experimental animals

Ankita

Deepti

Nilam

2014

Pharmaceutical Biology

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“…However, some studies suggest that metformin may have some renoprotective activity [52]. Metformin is suggested to provide protection against STZ induced nephropathy by restoration of biochemical alterations and modulation of oxidative stress [53]. Hence, the effect of metformin on decreasing creatinine level may be due to restoration of biochemical alterations and/or modulation of oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Anti-Hyperglycemic Effect of Different Fractions of Annona Reticulata Leaf

Rout

Kar

Maharana

2016

Asian J Pharm Clin Res

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Context: Several species of the genus Annona were reported to have hypoglycemic properties and this makes Annona reticulata Linn. (Annonaceae) an interesting plant for investigating its anti-hyperglycemic potential.Objective: Different fractions prepared from hydro-alcoholic extract of A. reticulata leave were investigated for their blood glucose lowering effect on Streptozotocin (STZ) induced hyperglycemic rats.Methods: Ethyl acetate, methanol, and residual fractions (at dose level of 100 mg/kg by oral route) prepared from the hydro-alcoholic extract of A. reticulata leave were administered for 14 consecutive days to STZ induced hyperglycemic rats for evaluation of their anti-hyperglycemic potential. Anti-hyperglycemic potential was assessed by observation of a decrease in fasting blood glucose level. Results:The studies revealed that ethyl acetate fraction decreased the blood glucose level of hyperglycemic rats from 447.67 to 234.17 mg/dL and is significant (p<0.001) when compared with diabetic control group. The residual fraction and methanolic fraction decreased blood glucose level from 417.83 to 402.50 mg/dL and 432.33 to 371.67 mg/dL respectively but not significant when compared with the diabetic control group. Standard drug metformin (dose 300 mg/kg) reduced the blood glucose level from 447.33 to 219.50 mg/dL. Discussion: Ethyl acetate fraction at tested dose level was capable not only to control the elevated blood glucose level but also able to attenuate certain secondary parameters associated with STZ induced hyperglycemia. Conclusion:This study suggested that the ethyl acetate fraction prepared from hydro-alcoholic extract of A. reticulata leave exhibit potential antihyperglycemic property in the tested experimental models and should be investigated further.

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“…Hasta ahora, se conoce poco el alcance de tal efecto en los pacientes con diabetes tipo 2. Otro estudio, en ratas con nefropatía diabética inducida por estreptozotocina, mostró que el tratamiento de la afectación renal con metformina normalizó todos los cambios bioquímicos y los depósitos de energía en el tejido renal 28 . A nivel transcripcional, el suministro de metformina causó una restauración significativa en los valores del ácido ribonucleico mensajero (mRNA), inducida por el estrés oxidativo, mientras que ocurría inhibición en los genes proinflamatorios, como el del factor alfa de necrosis tumoral y de la interleuquina-6 18 .…”

Section: Diabetes Enfermedad Renal Y Metforminaunclassified

Metformina en enfermedad renal diabética: estado actual

Arocha-Rodulfo¹,

Maini²

2017

Rev. Colomb. Nefrol.

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El tratamiento actual dirigido a retardar la progresión de la enfermedad renal diabética, incluye un control intensivo de la glucemia con control óptimo de la hipertensión arterial, bloqueo del sistema renina-angiotensina-aldosterona junto a otras intervenciones multifactoriales. Sin embargo, la protección renal, a pesar de éstas medidas, no se alcanza en su totalidad, habiendo escasos estudios donde se analice el efecto nefroprotector de las drogas antihiperglucemiantes mas allá de su efecto hipoglucemiante.Este artículo analiza la información existente sobre el estatus actual de la metformina en la enfermedad renal diabética.

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Metformin attenuates streptozotocin-induced diabetic nephropathy in rats through modulation of oxidative stress genes expression

Cited by 157 publications

References 49 publications

Flavonoid rich fraction ofPunica granatumimproves early diabetic nephropathy by ameliorating proteinuria and disturbed glucose homeostasis in experimental animals

Flavonoid rich fraction ofPunica granatumimproves early diabetic nephropathy by ameliorating proteinuria and disturbed glucose homeostasis in experimental animals

Anti-Hyperglycemic Effect of Different Fractions of Annona Reticulata Leaf

Metformina en enfermedad renal diabética: estado actual

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