Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

Abstract

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

Use of essential oils in combination with fibrolytic enzymes to decrease in vitro ruminal methane production

“…Previous studies showed that higher ivGP was observed in the early incubation intervals with exogenous enzyme treatment of lucerne forage (Colombatto et al, 2007), sugarcane leaves and sorghum straw (Elghandour et al, 2013), and in a grass hay/concentrate substrate (Giraldo et al, 2008). Higher ivGP has been used to validate the effect of fibrolytic enzyme in improving the digestibility of feedstuffs (Cantet et al, 2016). Fibrolytic enzymes act on the slowly but progressively fermentable fraction of feed DM (largely the NDF and ADF) through reduction in cell wall thickness (Van de Vyer & Cruywagen, 2013), allowing rumen microbes and fermentation enzymes an easier access to plant cell constituents (Pinos-Rodríguez et al, 2008).…”