Fruit waste (FW), mainly from agroindustry, is currently left behind in landfills despite its rich composition. The bioactive compounds (e.g., oils, polyphenols), carbohydrates, and lignin present in this biomass type require comprehensive characterization (i.e., identification and quantification) before they can be used as raw materials in biorefineries. This review collected information from scientific papers on FW compositional analysis methods and characterization data; the information needs to be compiled in a systematic, standardized, and comprehensive way to understand and quantify the true potential of FW as feedstocks for biorefineries. The information gathered in this review allowed us to identify the biomass fractions that could be valorized further depending on the kind of FW (peels, seeds, or seed vessels, and pomace or mixed residues). Fruit waste differs from conventional lignocellulosic biomass due to the presence of higher amounts (>5%) of extractives – pectin, and starch. This review describes current compositional analysis methodologies to identify possible strengths and weaknesses that could affect the adequate selection of valorization platforms. As no current methodology allows the composition of FW to be described thoroughly, this work identifies procedures applicable to biorefineries that use FW. Possible improvements are suggested to fill methodological gaps in the quantification of samples with large amounts of extractives and pectin. The standardization of methods for FW's quantification is fundamental for the adequate integration of different valorization platforms into biorefineries. It is essential to consider all the substances present in FW to exploit fully their potential for new value‐added molecules, including oils, polyphenols, and pectin.