Methane production of two roughage and total mixed ration as influenced by cellulase and xylanase enzyme addition

Gemeda, Belete Shenkute; Hassen, Abubeker

doi:10.1590/0103-9016-2014-0155

Cited by 8 publications

(6 citation statements)

References 22 publications

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“…Interestingly, higher abundance of Succinivibrio , which was the reason of low methane production from hindgut of Tammar wallabies ( Pope, 2011 ), was also observed in the inoculum incubated with cassava residues. This was also consistent with the conclusion that the ruminal methane production is always lower by feeding low fiber feedstuffs to ruminants ( Gemeda and Hassen, 2015 ).…”

Section: Discussionsupporting

confidence: 91%

Ruminal fermentation and microbial community differently influenced by four typical subtropical forages in vitro

et al. 2018

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The present study evaluated the effects of 4 typical subtropical forages on ruminal microbial community composition to formulate a better diet for buffalo. Corn straw silage, elephant grass, cassava residues and sugarcane tail silage were used as substrates for in vitro fermentation. Eight replicates were set up for every substrate, and fermentation was carried out in a 100-mL glass syringe, using buffalo rumen inoculum. Every replicate was anaerobically dispensed with 10 mL of rumen inoculum, 20 mL of McDougall's buffer and 200 mg of dried substrate, and placed in a water bath at 39 °C. Gas production was recorded at 0, 2, 6, 12, 24, 36, 48 and 72 h of incubation. After 24 h, fermentation was ceased for 4 replicates and samples were collected. Volatile fatty acids (VFA) concentrations were measured using gas chromatography. Microbial populations were quantified using quantitative real-time PCR (qRT-PCR), and microbial community was analyzed using high throughput sequencing technology. The results showed, cassava residues as substrate had the highest gas production, acetate, propionate and total VFA concentrations (P < 0.05), and corn straw silage had the lowest acetate:propionate ratio (P < 0.05). The lowest numbers of fungi, Ruminococcus albus and Fibrobacter succinogenes, and the highest number of protozoa were observed with cassava residues (P < 0.05). The least abundances of bacterial phyla Firmicutes, Bacteroidetes and genus Prevotella, and substantially higher abundance of phylum proteobacteria (56%) and genus Succinivibrio (52%) were observed with cassava residues. The most abundances of Methanobrevibacter gottschalkii and Entodinium were observed with cassava residues. Spearman's correlations analysis showed, Succinivibrio had strong positive correlations with propionate, butyrate, Metadinium and M. gottschalkii, indicating fermentation products were related to microbial community. In conclusion, incubation with cassava residues resulted in lower number of fiber degrading microbes but higher protozoal population because of its low fiber contents. The microbial community was highly altered by in vitro incubation with cassava residues, whereas remained similar for the other 3 high fiber containing substrates.

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Section: Discussionsupporting

confidence: 91%

Ruminal fermentation and microbial community differently influenced by four typical subtropical forages in vitro

et al. 2018

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“…Experiment 1-in vitro fermentation: The technique of in vitro batch culture in 100 ml serum bottle was performed (3 runs) as per Gemeda and Hassen (2015). The enzymatic supplementation was performed with 2 different methods according to the enzymes form.…”

Section: Methodsmentioning

confidence: 99%

Effect of combining exogenous fibrolytics enzymes supplementation with alkali and acid pre-treatments on wheat straw hydrolysis and ruminal fermentation

Jabri

Abid

Yaich

et al. 2019

Indian J of Anim Sci

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The present study was undertaken to evaluate the efficacy of exogenous fibrolytics enzymes (EFE) to improve the in vitro cell wall hydrolysis, ruminal fermentation and digestibility of untreated (WS) and chemically pretreated wheat straw with NaOH (SWS), urea (UWS), and diluted H2SO4 (AWS). An in vitro gas production study during 96 h of incubation and an in vitro enzymatic hydrolysis during 20 h was conducted. The first EFE was a mixture (1:1, v/v) of cellulase and xylanase (Dyadic complex), applied at increasing doses (1, 2, 5 and 10 Î¼l/g DM). The second EFE (MaxFiber complex) was also applied at increasing dose (0.5, 1, 2 and 4 mg/g DM). The rate and the extent of the in vitro gas production (GP) of WS improved with both EFE supplementation especially with the optimal doses D1 and M2. In association with NaOH pre-treatment, the EFE failed to have any effect on wheat straw digestibility. In contrast, the urea pre-treatment seems to have a synergetic effect with EFE on ruminal utilization by accelerating the fermentation process. However, for AWS an antagonist effect was detected with both EFE. Moreover, the in vitro enzymic hydrolysis indicated a linear positive effect of EFE on reducing sugar release for almost all substrates and an increase in dry matter losses for only WS and AWS. These results revealed that the positive effect of EFE supplementation effect depended on the type of chemical pre-treatments and it was detected only for WS and UWS.

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“…The higher CH 4 production (g/kg DMI) reported in Table 2 for Treatments 1 and 2 could be due to the associated higher concentrations of cell wall components (NDF and ADF) and lower concentrations of CP and NFC compared to T 3 and T 4. Gemeda and Hassen (2015) reported a negative correlation between in vitro CH 4 production and the NFC, CP and ADIN concentrations in feed samples. These authors also reported a positive correlation between forage fibre concentration and in vitro CH 4 production.…”

Section: Dry Matter Intake Digestion and Methane Productionmentioning

confidence: 98%

Methane emissions from sheep fed Eragrostis curvula hay substituted with Lespedeza cuneata

et al. 2020

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Context Reducing emissions of greenhouse gases from livestock production systems is a global research priority. Forages that contain condensed tannins, such as the perennial legume Lespedeza cuneata, may help to reduce ruminant methane (CH4) emissions. Aims The objective of this study was to investigate the effect of feeding different levels of L. cuneata hay on feed intake and enteric CH4 emissions of sheep fed a basal diet of subtropical Eragrostis curvula hay. Methods Four adult ruminally cannulated Dohne Merino wethers with initial bodyweight of 65.5 ± 3.5 kg were used in the experiment in a 4 × 4 Latin square design. The four experimental treatments were E. curvula hay substituted with 0%, 30%, 60% and 90% L. cuneata hay. Each of four experimental periods lasted 27 days, which consisted of a 14-day adaptation period, a 7-day digestibility trial, and a 6-day CH4-measurement period. During the 6-day CH4-measurement period, CH4 emissions were measured continuously over a 24-h period by using an open circuit respiration system. Key results Dry matter intake (DMI, g/kg W0.75) was higher (P < 0.05) for sheep receiving 60% and 90% L. cuneata than 0% and 30% L. cuneata (77.33 and 84.67 g/kg W0.75 vs 62.96 and 62.71 g/kg W0.75). The increase in DMI corresponded with a linear increase in DM digestibility of the experimental treatments from 38% to 45% as the level of L. cuneata substitution increased. Methane yield was not influenced (P > 0.05) by 30% inclusion of L. cuneata (17.6 g CH4/kg DMI) but decreased (P < 0.05) as the inclusion level increased to 60% and 90% (13.8 and 14.3 g CH4/kg DMI). Conclusions Inclusion of L. cuneata hay in a diet based on E. curvula hay improved diet digestibility, and led to increased concentrations of crude protein, neutral detergent fibre and non-fibre carbohydrates. Substituting E. curvula hay with 60% L. cuneata on a DM basis resulted in the greatest reduction in CH4 yield of 21.4% compared with a diet of 100% E. curvula. Implications The results suggest that L. cuneata has the potential to reduce CH4 yield and possibly increase production from sheep by improving diet DM digestibility and through improved DMI.

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Methane production of two roughage and total mixed ration as influenced by cellulase and xylanase enzyme addition

Cited by 8 publications

References 22 publications

Ruminal fermentation and microbial community differently influenced by four typical subtropical forages in vitro

Ruminal fermentation and microbial community differently influenced by four typical subtropical forages in vitro

Effect of combining exogenous fibrolytics enzymes supplementation with alkali and acid pre-treatments on wheat straw hydrolysis and ruminal fermentation

Methane emissions from sheep fed Eragrostis curvula hay substituted with Lespedeza cuneata

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