Methanocaldococcus villosus sp. nov., a heavily flagellated archaeon that adheres to surfaces and forms cell–cell contacts

Bellack, Annett; Huber, Harald; Rachel, Reinhard; Wanner, Gerhard; Wirth, Reinhard

doi:10.1099/ijs.0.023663-0

Cited by 86 publications

(67 citation statements)

References 28 publications

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“…3C and D). 21 On most of the examined materials, the cells were spread evenly over the entire surface rather than forming large aggregates. It became obvious that the cells interacted extensively via their flagella.…”

Section: Adhesion Of the P Furiosus/m Villosus Coculture To Surfacesmentioning

confidence: 99%

“…The fact that P. furiosus is not able to adhere to glass surfaces had already been shown previously and was confirmed here. 17,25 M. villosus was described to attach to glass cover slips 21 and somehow seems to prime the surface for adhesion of P. furiosus, since in coculture both species were observed to attach to this material. Synthetic polymers like PTFE and silicon allowed the adherence of both species in pure culture as well as in coculture.…”

Section: Adhesion Of the P Furiosus/m Villosus Coculture To Surfacesmentioning

confidence: 99%

“…Methanocaldococcus jannaschii (JAL-1 T , DMS 2661), 19 Methanocaldococcus infernus (ME T , DSM 11812) 20 , Methanocaldococcus villosus (KIN24-T80 T , DSM 22612) 21 and Methanotorris igneus (Kol 5 T , DSM 5666). 22 MGG medium (standard methanogen medium with a ionic composition similar to sea salts) 21 supplemented with 0.1% yeast extract and 0.25% peptone was used for cultivation of cocultures that were grown in 120 mL bottles containing 20 mL anaerobic medium at 85 °C with agitation.…”

mentioning

confidence: 99%

“…17,21,25 Different materials were added to ½ SME medium which was inoculated and incubated at 85 °C for 20 h until stationary growth phase was surely reached. 24 Gas phases were N 2 /CO 2 for P. furiosus, H 2 /CO 2 for M. villosus and N 2 /H 2 /CO 2 (65/15/20, v/v/v) for the coculture.…”

mentioning

confidence: 99%

“…22 MGG medium (standard methanogen medium with a ionic composition similar to sea salts) 21 supplemented with 0.1% yeast extract and 0.25% peptone was used for cultivation of cocultures that were grown in 120 mL bottles containing 20 mL anaerobic medium at 85 °C with agitation. Growth was monitored by cell counting using a Thoma cell counting chamber (depth 0.02 mm) and a Nikon Microphot epi-fluorescence microscope.…”

mentioning

confidence: 99%

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Positive, Neutral and Negative Interactions in Cocultures between Pyrococcus furiosus and Different Methanogenic Archaea

et al. 2012

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Abstract:The model organism Pyrococcus furiosus has recently been reported to interact with Methanopyrus kandleri in coculture, suggesting a H 2 symbiosis. In the current study we further investigated this hypothesis by growing P. furiosus with four other hyperthermophilic methanogens providing evidence that the organisms did not only exert positive effects (P. furiosus/ Methanocaldococcus villosus and P. furiosus/Methanocaldococcus infernus) on each other, but also neutral (P. furiosus/Methanocaldococcus jannaschii) and even inhibitory interactions (P. furiosus/Methanotorris igneus) were detected suggesting interspecies relationships not only based on H 2 symbiosis. Using various microscopic techniques we further analyzed the coculture with the highest positive interactions (P. furiosus/ M. villosus) concerning its growth behavior on various surfaces, which turned out to be in stark contrast to the previous reported coculture of P. furiosus/M. kandleri. This communication provides new insights into possible interactions of extremophilic Archaea in cocultures and again raises the question if and how hyperthermophilic Archaea communicate besides metabolic intermediates like H 2 .

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Section: Adhesion Of the P Furiosus/m Villosus Coculture To Surfacesmentioning

confidence: 99%

Section: Adhesion Of the P Furiosus/m Villosus Coculture To Surfacesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Positive, Neutral and Negative Interactions in Cocultures between Pyrococcus furiosus and Different Methanogenic Archaea

et al. 2012

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Archaeal Cells

Klingl

Flechsler

Heimerl

et al. 2013

Encyclopedia of Life Sciences

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At a first glance, Archaea are structurally seen quite similar to Bacteria, and for a long time they were named ‘Archaebacteria’. They can form cocci, rods, spirals or irregular shaped cells and are equally sized as Bacteria. Together they are referred to as ‘Prokaryotes’ because neither Archaea nor Bacteria have a nucleus. Although this term indeed might be helpful in habitual language use, it does not refer to a phylogenetic group. In fact, transcription and translation machineries of Archaea have much more in common with eukaryotic cells than with Bacteria. In addition, there are many features that remain characteristic for Archaea, given by the fact that many representatives live and thrive under extreme environmental conditions. In the review, the authors give a comprehensive overview about the stunning diversity of structural features in archaeal cell envelopes, membranes and cell appendages. Key Concepts: By application of recently developed PCR techniques, Archaea can be found in almost every habitat and sometimes are even more abundant than bacteria. Archaea show special adaptations to their sometimes extreme environments, like caldarchaeols, which are more stable at high temperatures. Like Bacteria, Archaea are also surrounded by a lipid bilayer, but in the latter case the lipid moiety consists of C 5 ‐isoprenoid units that are coupled to glycerol via ether bonds at (sn)‐2,3 positions of the glycerol. Cell walls can be as simple as a proteinaceous surface layer or as complicated as in some methanogens with multiple layers, additional sheats enclosing several cells and even more complex cell wall compounds. Archaea exhibit a broad variety in cell appendages that are different to bacterial ones in fine structure, composition, biosynthesis and anchorage in the cell.

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Archaeal Cells

Chadha¹,

Seydel²,

Klingl³

2020

Encyclopedia of Life Sciences

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At a first glance, Archaea are quite similar to Bacteria on a structural level, and for a long time they were named ‘Archaebacteria’. They can form cocci, rods, spirals or irregular shaped cells and are equally sized as Bacteria. Together they are referred to as ‘Prokaryotes’ because neither Archaea nor Bacteria possess a nucleus. Although this term indeed might be helpful in habitual language use, it does not refer to a phylogenetic group. In fact, transcription and translation machineries of Archaea have much more in common with eukaryotic cells than with Bacteria. In addition, there are many features that remain characteristic for Archaea, given by the fact that many representatives live and thrive under extreme environmental conditions. Key Concepts By application of respective PCR techniques, Archaea can be found in almost every habitat and sometimes are even more abundant than bacteria. Archaea show special adaptations to their sometimes extreme environments, like caldarchaeols, which are more stable at high temperatures. Like Bacteria, Archaea are also surrounded by a lipid bilayer, but in the latter case, the lipid moiety consists of C 5 ‐isoprenoid units that are coupled to glycerol via ether bonds at (sn)‐2,3 positions of the glycerol. Cell walls can be as simple as a proteinaceous surface layer or as complicated as in some methanogens with multiple layers, additional sheaths enclosing several cells and even more complex cell wall compounds. Archaea exhibit a broad variety in cell appendages that are different from bacterial ones in fine structure, composition, biosynthesis and anchorage in the cell.

show abstract

Methanocaldococcus villosus sp. nov., a heavily flagellated archaeon that adheres to surfaces and forms cell–cell contacts

Cited by 86 publications

References 28 publications

Positive, Neutral and Negative Interactions in Cocultures between Pyrococcus furiosus and Different Methanogenic Archaea

Positive, Neutral and Negative Interactions in Cocultures between Pyrococcus furiosus and Different Methanogenic Archaea

Archaeal Cells

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