“…The archaea 16S rRNA gene was amplied using the barcoded primer pair ARC-787f (5 0 -ATTAGA-TACCCSBGTAGTCC-3 0 ) and ARC-1059r (5 0 -GCCATG-CACCWCCTCT-3 0 ). 32 Sequencing of archaeal amplicons was performed on Illumina MiSeq at Genenergy, Shanghai, China. The Illumina sequencing raw data were deposited in the NCBI Sequence Read Archive database (accession no.…”
Section: Dna Extraction and Illumina 16s Rrna Gene Amplicon Sequencingmentioning
Physical structures of sludge are critical factors determining the performance of the anaerobic digestion process, especially for the rate-limiting step, methanogenesis.
“…The archaea 16S rRNA gene was amplied using the barcoded primer pair ARC-787f (5 0 -ATTAGA-TACCCSBGTAGTCC-3 0 ) and ARC-1059r (5 0 -GCCATG-CACCWCCTCT-3 0 ). 32 Sequencing of archaeal amplicons was performed on Illumina MiSeq at Genenergy, Shanghai, China. The Illumina sequencing raw data were deposited in the NCBI Sequence Read Archive database (accession no.…”
Section: Dna Extraction and Illumina 16s Rrna Gene Amplicon Sequencingmentioning
Physical structures of sludge are critical factors determining the performance of the anaerobic digestion process, especially for the rate-limiting step, methanogenesis.
“…Fig. 5 shows that the VFA concentration during the experiment varies between 200−320 mg/L, this concentration shows a good operation of the digester [32]. These results we can conclude that in the case of recycled paper mill wastewater, the digester is operating well when the VFA in the outlet effluent are less than 0.4 g/L.…”
Section: Resultsmentioning
confidence: 77%
“…Moreover, VFA accumulation is a simple way to detect a biochemical dysfunction of the anaerobic digestion process. Monitoring these compounds is, therefore, a way of controlling the functioning of the digesters [31,32]. Fig.…”
HighlightsUASB was proposed for RPMW anaerobic treatement under mesophilic conditions.Start-up up of the UASB reactor was completed in the 24 days.80,76 % COD and 90 % TS were removed at an OLR of 7,27 g COD/L d.At optimal OLR of 8.31 g COD/L d was required to produce 62.51 L/d biogas.For HRT 15.14 h, after reactor start-up 80.63 % of COD was removed.The average biogas composition was 72.98 % CH4, 19.76 % CO2, 0.9 % O2.
“…Today, energy demand is constantly increasing due to the intense consumption of natural resources such as natural gas, oil and coal in the sectors such as industries, residences etc (Rathaur, 2018). Being dependent on fossil fuels as primary energy source has caused global climate change, environmental degradation and human health problems.…”
Grass is a highly desirable substrate for anaerobic digestion because of its higher biodegradability and biogas/methane yield. It contains a large amount of organic matter, which can be digested anaerobically to produce biogas. Anaerobic co-digestion of grass, cow manure and sludge was studied under mesophilic conditions for 65 days. Experiments were performed on a feed ratio of grass/manure 5, 10, 15, 20, 25%, respectively. During the experiments the volume and concentration of biogas and methane were recorded daily. The maximum cumulative biogas and methane yield was obtained as 331.75 mLbiogas/gVS and 206.64 mLCH4/gVS for 25% ratio. Also, the results of the experiments were tested on the three different kinetics model which are the first order kinetic model, modified Gompertz model and Logistics model. As a result of the study, it was found that by using grass waste 1.2.109 kWh/year electricity may be produced and 1.106 tons/year CO2 greenhouse gas emission may be reduced.
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