Methanogenic biodegradation of paraffinic solvent hydrocarbons in two different oil sands tailings

Shahimin, Mohd Faidz Mohamad; Siddique, Tariq

doi:10.1016/j.scitotenv.2017.01.038

Cited by 32 publications

(34 citation statements)

References 29 publications

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“…The abundance of Anaerolineaceae was found to be increased in the alkane-amended enrichment cultures. Microorganisms affiliated to the family Anaerolineaceae have been detected in a vast number of methanogenic alkane-degrading enrichment cultures and were implicated to be responsible for alkane activation in these cultures (Liang et al 2015;Liang et al 2016;Mohamad Shahimin et al 2016;Mohamad Shahimin and Siddique 2017). However, Anaerolineaceae-related assA genes were not detected in the current culture, consistent with previous studies (Liang et al 2016;Mohamad Shahimin et al 2016).…”

Section: Key Members Involved In Methanogenic N-alkane Degradationsupporting

confidence: 88%

“…Sherry et al observed that Smithella was significantly enriched in both the weathered and non-weathered oil-amended (containing C 5 to C 10 n-alkanes) methanogenic enrichment cultures, indicating that Smithella can utilize low molecular weight n-alkanes (Sherry et al 2014). Novel members of the family Peptococcaceae were identified to be the primary degraders in several methanogenic short alkane-degrading (C 5 to C 10 ; n-, iso-and cyclo-alkanes) enrichment cultures derived from oil sands tailings ponds (Abu Laban et al 2015;Mohamad Shahimin et al 2016;Mohamad Shahimin and Siddique 2017;Siddique et al 2015;Tan et al 2014;Tan et al 2013). By investigating methanogenic biodegradation of C 7 to C 8 iso-alkanes, Abu Laban et al proposed a novel family Peptococcaceae activated these substrates by addition to fumarate with the detection of high abundance of Peptococcaceae, Peptococcaceae-related assA gene and fumarate addition metabolites of C 7 to C 8 iso-alkans (Abu Laban et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Methanogenic biodegradation of C9 to C12n-alkanes initiated by Smithella via fumarate addition mechanism

Zhou

Mbadinga

et al. 2020

AMB Expr

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In the present study, a methanogenic alkane-degrading (a mixture of C 9 to C 12 n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C 9 to C 12 alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.

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Section: Key Members Involved In Methanogenic N-alkane Degradationsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Methanogenic biodegradation of C9 to C12n-alkanes initiated by Smithella via fumarate addition mechanism

Zhou

Mbadinga

et al. 2020

AMB Expr

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“…Compared to naphtha, paraffinic diluent is relatively simple and is composed primarily of C 5 and C 6 nand iso-alkanes [8,22]. Analysis of paraffinic diluent found that it contained 24 wt% n-pentane, 11 wt% n-hexane, and 49 wt% iso-alkanes (2-methylbutane, 2-methylpentane, and 3-methylpentane) [26].…”

Section: Paraffinic Fttmentioning

confidence: 99%

“…The Syncrude naphtha extraction method requires five minutes of shaking, while Siddique et al's naphtha and alkane extraction procedures involved 10 and 30 min of shaking, respectively. Paraffinic diluent has been extracted from tailings samples using methanol as the solvent with a 10:1 solvent to tailings ratio, where samples underwent 30 min of shaking prior to settling and subsequent purge-and-trap GC analysis [22,26]. The use of different diluent types (paraffinic versus naphtha) should not have a significant impact on the extraction procedure, as both fall within a low to moderate carbon number range.…”

Section: Chemical Analysis Of Ftt Diluent 41 Diluent Extraction Techniquesmentioning

confidence: 99%

A Deep Look into the Microbiology and Chemistry of Froth Treatment Tailings: A Review

et al. 2021

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In Alberta’s Athabasca oil sands region (AOSR), over 1.25 billion m3 of tailings waste from the bitumen extraction process are stored in tailings ponds. Fugitive emissions associated with residual hydrocarbons in tailings ponds pose an environmental concern and include greenhouse gases (GHGs), reduced sulphur compounds (RSCs), and volatile organic compounds (VOCs). Froth treatment tailings (FTT) are a specific type of tailings waste stream from the bitumen froth treatment process that contains bioavailable diluent: either naphtha or paraffins. Tailings ponds that receive FTT are associated with the highest levels of biogenic gas production, as diverse microbial communities biodegrade the residual diluent. In this review, current literature regarding the composition, chemical analysis, and microbial degradation of FTT and its constituents is presented in order to provide a more complete understanding of the complex chemistry and biological processes related to fugitive emissions from tailings ponds receiving FTT. Characterizing the composition and biodegradation of FTT is important from an environmental perspective to better predict emissions from tailings ponds and guide tailings pond management decisions.

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“…Both acetoclastic and hydrogenotrophic methanogenic archaea are found in hydrocarbon-contaminated soil and sediments. Nonetheless, their ratio depends on the composition of the entire community (Siddique et al, 2011;Wawrik et al, 2016;Mohamad Shahimin and Siddique, 2017a).…”

Section: Syntrophy In Hydrocarbon-degrading Microbial Communitiesmentioning

confidence: 99%

New Frontiers of Anaerobic Hydrocarbon Biodegradation in the Multi-Omics Era

et al. 2020

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The accumulation of petroleum hydrocarbons in the environment substantially endangers terrestrial and aquatic ecosystems. Many microbial strains have been recognized to utilize aliphatic and aromatic hydrocarbons under aerobic conditions. Nevertheless, most of these pollutants are transferred by natural processes, including rain, into the underground anaerobic zones where their degradation is much more problematic. In oxic zones, anaerobic microenvironments can be formed as a consequence of the intensive respiratory activities of (facultative) aerobic microbes. Even though aerobic bioremediation has been well-characterized over the past few decades, ample research is yet to be done in the field of anaerobic hydrocarbon biodegradation. With the emergence of high-throughput techniques, known as omics (e.g., genomics and metagenomics), the individual biodegraders, hydrocarbon-degrading microbial communities and metabolic pathways, interactions can be described at a contaminated site. Omics approaches provide the opportunity to examine single microorganisms or microbial communities at the system level and elucidate the metabolic networks, interspecies interactions during hydrocarbon mineralization. Metatranscriptomics and metaproteomics, for example, can shed light on the active genes and proteins and functional importance of the less abundant species. Moreover, novel unculturable hydrocarbon-degrading strains and enzymes can be discovered and fit into the metabolic networks of the community. Our objective is to review the anaerobic hydrocarbon biodegradation processes, the most important hydrocarbon degraders and their diverse metabolic pathways, including the use of various terminal electron acceptors and various electron transfer processes. The review primarily focuses on the achievements obtained by the current high-throughput (multi-omics) techniques which opened new perspectives in understanding the processes at the system level including the metabolic routes of individual strains, metabolic/electric interaction of the members of microbial communities. Based on the multi-omics techniques, novel metabolic blocks can be designed and used for the construction of microbial strains/consortia for efficient removal of hydrocarbons in anaerobic zones.

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Methanogenic biodegradation of paraffinic solvent hydrocarbons in two different oil sands tailings

Cited by 32 publications

References 29 publications

Methanogenic biodegradation of C9 to C12n-alkanes initiated by Smithella via fumarate addition mechanism

Methanogenic biodegradation of C9 to C12n-alkanes initiated by Smithella via fumarate addition mechanism

A Deep Look into the Microbiology and Chemistry of Froth Treatment Tailings: A Review

New Frontiers of Anaerobic Hydrocarbon Biodegradation in the Multi-Omics Era

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