Methanogenic Microorganisms in Industrial Wastewater Anaerobic Treatment

Vítězová, Monika; Kohoutová, Anna; Vítěz, Tomáš; Hanišáková, Nikola; Kushkevych, Ivan

doi:10.3390/pr8121546

Cited by 58 publications

(32 citation statements)

References 137 publications

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“…In addition, the anaerobic aquifer contained methanogens (Methanolinea sp. ), which could convert the added auxiliary substrate into methane (Vítězová et al, 2020).Therefore, during the process of reductive dechlorination, a large amount of methane was also produced .Dehalococcoides was found in both Port 3 and Port 9 in the aquifer, indicating that the reductive The aerobic simulated aquifer contained three kinds of methanotrophs: Methylococcaceae bacterium, Methylosarcina fibrata strain AML-C10, and Verrucomicrobiaceae bacterium DC2c-37.This was why in the aerobic stage, methane was almost 100% consumed, producing sMMO degrading enzymes, and cometabolizing TCE, cDCE and VC at the same time. Since methanotrophs are sensitive to inhibition of organic compounds, mixed cultures are more stable and suitable than pure cultures for th environmental remediation (Fogel et al, 1986).…”

Section: Resultsmentioning

confidence: 99%

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

Chen¹,

Chin²,

Yang³

et al. 2021

JEB

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Aim: This study explores anaerobic/aerobic biodegradation efficiencies of aerobic cometabolism with methanotrophs when contaminants trichloroethylene (TCE) and cis-1,2-dichloroethylene (cDCE) are present individually or in tandem. Methodology: Batch tests and an anaerobic/aerobic column system were used to simulate saturated, contaminated aquifers. A brown glass bottle with an effective volume of 44 m l-1 was prepared for the batch test. An integrated one-dimensional sequential anaerobic/aerobic column system was used to simulate the accumulative intermediates such as TCE, cDCE and VC caused by incomplete degradation of PCE during the upgradient anaerobic stage in the saturated aquifer. In the downgradient aquifer, aerobic cometabolism was employed to degrade the intermediates. Methanotrophs in the aerobic aquifer were inoculated to degrade the by-products of incomplete degradation of PCE by aerobic cometabolism. Results: In the batch test, biodegradation of TCE was significantly inhibited by cDCE. However, biodegradation of cDCE was not significantly inhibited by TCE. In the simulated aquifer test, aerobic cometabolism completely degraded intermediates (TCE, cDCE, and VC) produced by incomplete anaerobic degradation of tetrachloroethylene (PCE). The results showed that methane, a by-product of anaerobic reductive dechorination of PCE, was used as a primary substrate for aerobic degradation, at a utilization rate of almost 100%. Interpretation: Biodegradation of TCE was significantly inhibited by cDCE. Bioremediation should have sufficient oxygen and methane at aerobic stage to ensure that chlorinated ethenes fully mineralize.

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Section: Resultsmentioning

confidence: 99%

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

Chen¹,

Chin²,

Yang³

et al. 2021

JEB

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“…Methanogenesis is detectable, among other methods, by the release of methane. In the intestines, fermentation produces H 2 , which can be used by SRB or methanogens [50] for methane production [51].…”

Section: Large Intestine Of Humans and Animalsmentioning

confidence: 99%

Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats

Kushkevych

Hýžová

Vítězová

et al. 2021

IJMS

Self Cite

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This paper is devoted to microscopic methods for the identification of sulfate-reducing bacteria (SRB). In this context, it describes various habitats, morphology and techniques used for the detection and identification of this very heterogeneous group of anaerobic microorganisms. SRB are present in almost every habitat on Earth, including freshwater and marine water, soils, sediments or animals. In the oil, water and gas industries, they can cause considerable economic losses due to their hydrogen sulfide production; in periodontal lesions and the colon of humans, they can cause health complications. Although the role of these bacteria in inflammatory bowel diseases is not entirely known yet, their presence is increased in patients and produced hydrogen sulfide has a cytotoxic effect. For these reasons, methods for the detection of these microorganisms were described. Apart from selected molecular techniques, including metagenomics, fluorescence microscopy was one of the applied methods. Especially fluorescence in situ hybridization (FISH) in various modifications was described. This method enables visual identification of SRB, determining their abundance and spatial distribution in environmental biofilms and gut samples.

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“…Notably, a diverse archaeal community forming almost one-tenth of the prokaryotic community was present in the effluent of this WWTP. The WWTP effluent proved to be a significant source of the Euryarchaeotal genera Methanosarcina and Methanothrix, which have been found in cold-climate WWTPs [31] as well as anaerobic industrial WWTPs [32]. The WWTP effluent also boasted a diverse pathogenic community, which, unlike large-scale WWTPs [30], was dominated by environmental bacteria that include nosocomial pathogens (i.e., Pseudomonas aeruginosa).…”

Section: The Characteristics Of Wwtp Effluent and Its Effect On The Pmentioning

confidence: 99%

The Effect of the Effluent from a Small-Scale Conventional Wastewater Treatment Plant Treating Municipal Wastewater on the Composition and Abundance of the Microbial Community, Antibiotic Resistome, and Pathogens in the Sediment and Water of a Receiving Stream

Tiirik

Nõlvak

Truu

et al. 2021

Water

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The effluents of wastewater treatment plants (WWTPs) are major contributors of nutrients, microbes—including those carrying antibiotic resistance genes (ARGs)—and pathogens to receiving waterbodies. The effect of the effluent of a small-scale activated sludge WWTP treating municipal wastewater on the composition and abundance of the microbial community as well as the antibiotic resistome and pathogens in the sediment and water of the receiving stream and river was studied using metagenome sequencing and a quantitative approach. Elevated Bacteroidetes proportions in the prokaryotic community, heightened sulfonamide and aminoglycoside resistance determinants proportions, and an increase of up to three orders of magnitude of sul1–sul2–aadA–blaOXA2 gene cluster abundances were recorded in stream water and sediments 0.3 km downstream of a WWTP discharge point. Further downstream, a gradual recovery of affected microbial communities along a distance gradient from WWTP was recorded, culminating in the mostly comparable state of river water and sediment parameters 3.7 km downstream of WWTP and stream water and sediments upstream of the WWTP discharge point. Archaea, especially Methanosarcina, Methanothrix, and Methanoregula, formed a substantial proportion of the microbial community of WWTP effluent as well as receiving stream water and sediment, and were linked to the spread of ARGs. Opportunistic environmental-origin pathogens were predominant in WWTP effluent and receiving stream bacterial communities, with Citrobacter freundii proportion being especially elevated in the close vicinity downstream of the WWTP discharge point.

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Methanogenic Microorganisms in Industrial Wastewater Anaerobic Treatment

Cited by 58 publications

References 137 publications

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats

The Effect of the Effluent from a Small-Scale Conventional Wastewater Treatment Plant Treating Municipal Wastewater on the Composition and Abundance of the Microbial Community, Antibiotic Resistome, and Pathogens in the Sediment and Water of a Receiving Stream

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