Methanogens and Methanotrophs Show Nutrient-Dependent Community Assemblage Patterns Across Tropical Peatlands of the Pastaza-Marañón Basin, Peruvian Amazonia

Finn, Damien; Ziv-El, Michal C; Haren, Joost van; Park, Jin Gyoon; Águila-Pasquel, Jhon del; Muñoz, José David Urquiza; Cadillo‐Quiroz, Hinsby

doi:10.3389/fmicb.2020.00746

Cited by 40 publications

(74 citation statements)

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“…The steep decrease of CH 4 concentrations in surface levels but absence of a strong signature of CH 4 oxidation in the isotopic data suggest that CH 4 is lost from soils perhaps mainly through diffusion from water saturated soil to the atmosphere or through palms and trees as reported for this site (Van Haren and Cadillo-Quiroz, 2016). Aerobic oxidation of methane likely operates in QUI as methanotrophic sequences were detected in this and previous study (Finn et al, 2020), however, frequent near or above soil surface water table in this site likely limit this activity to dryer seasons or elevated soils. The minerotrophic, near-circumneutral pH, peat soils of BVA were characterized by relatively high NO 3 − concentrations in shallow soil layers, leading to lower C/N ratios and a higher nitrogen nutrient richness that in the other sites.…”

Section: Discussionsupporting

confidence: 57%

“…The determined thresholds of ∼10 μM in BVA and approximate <0.004 μM in SJO are above and below the N 2 O soil gas concentrations. This may contribute to the general emission pattern of higher CH 4 emissions from BVA peat and low CH 4 emissions from SJO peat ( Finn et al, 2020 ). We should note that the enrichments used for the incubations represented only a fraction of the microbial community present and active in-situ .…”

Section: Discussionmentioning

confidence: 99%

“…However, spatial and temporal CH 4 flux variability in most terrestrial ecosystems is high, particularly in the Amazon basin ( Bloom et al, 2010 ; Davidson et al, 2012 ), and with low modeling predictability ( Parker et al, 2018 ). Peatlands have been overlooked as strong CH 4 sources within tropical latitudes ( Sakabe et al, 2018 ; Tang et al, 2018a ; Wong et al, 2018 , 2020 ; Finn et al, 2020 ), including recently documented sites in the Peruvian Amazon ( Teh et al, 2017 ; Winton et al, 2017 ; Finn et al, 2020 ). Large swaths of areas holding tropical peatlands have been reported in the Amazon basin ( Gumbricht et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Microbial Communities and Interactions of Nitrogen Oxides With Methanogenesis in Diverse Peatlands of the Amazon Basin

et al. 2021

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Tropical peatlands are hotspots of methane (CH4) production but present high variation and emission uncertainties in the Amazon region. This is because the controlling factors of methane production in tropical peats are not yet well documented. Although inhibitory effects of nitrogen oxides (NOx) on methanogenic activity are known from pure culture studies, the role of NOx in the methane cycling of peatlands remains unexplored. Here, we investigated the CH4 content, soil geochemistry and microbial communities along 1-m-soil profiles and assessed the effects of soil NOx and nitrous oxide (N2O) on methanogenic abundance and activity in three peatlands of the Pastaza-Marañón foreland basin. The peatlands were distinct in pH, DOC, nitrate pore water concentrations, C/N ratios of shallow soils, redox potential, and 13C enrichment in dissolved inorganic carbon and CH4 pools, which are primarily contingent on H2-dependent methanogenesis. Molecular 16S rRNA and mcrA gene data revealed diverse and novel methanogens varying across sites. Importantly, we also observed a strong stratification in relative abundances of microbial groups involved in NOx cycling, along with a concordant stratification of methanogens. The higher relative abundance of ammonia-oxidizing archaea (Thaumarchaeota) in acidic oligotrophic peat than ammonia-oxidizing bacteria (Nitrospira) is noteworthy as putative sources of NOx. Experiments testing the interaction of NOx species and methanogenesis found that the latter showed differential sensitivity to nitrite (up to 85% reduction) and N2O (complete inhibition), which would act as an unaccounted CH4 control in these ecosystems. Overall, we present evidence of diverse peatlands likely differently affected by inhibitory effects of nitrogen species on methanogens as another contributor to variable CH4 fluxes.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microbial Communities and Interactions of Nitrogen Oxides With Methanogenesis in Diverse Peatlands of the Amazon Basin

et al. 2021

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“…The highest prokaryotic diversity occurs near the peat surface (Ong et al, 2015;Too et al, 2018) and decreases sharply with depth due to anoxia and lack of fresh organic inputs; at depth, Acidobacteria and Crenarchaeota become the dominant bacterial and archaeal taxa, respectively (Jackson et al, 2009). With depth, there is also an increased abundance of anaerobic methanogenic bacteria (notably Methanobacteriaceae) and methanotrophic bacteria (notably Methylocystaceae) which are favoured by persistently saturated conditions (Finn et al, 2020). Conversion of pristine conditions to drained secondary forest has been shown to cause large changes in prokaryotic communities, for example by favouring Grampositive bacteria over Gram-negative bacteria, which contribute to increased aerobic (vs. anaerobic) microbial activity and therefore more than doubled emissions of CO 2 (factor of 2.15; Dhandapani et al, 2019).…”

Section: B Elow-g Round B I Ota and Pe Atl And Fun C Tioningmentioning

confidence: 99%

Degradation of Southeast Asian tropical peatlands and integrated strategies for their better management and restoration

Mishra

Page

Cobb

et al. 2021

Journal of Applied Ecology

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1. About half of the world's tropical peatlands occur in Southeast (SE) Asia, where they serve as a major carbon (C) sink. Nearly 80% of natural peatlands in this region have been deforested and drained, with the majority under plantations and agriculture. This conversion increases peat oxidation which contributes to rapid C loss to the atmosphere as greenhouse gas emissions and increases their vulnerability to fires which generate regional smoke haze that has severe impacts on human health. Attempts at restoring these systems to mitigate environmental problems have had limited success.2. We review the current understanding of intact and degraded peatlands in SE Asia to help develop a way forward in restoring these ecosystems. As such, we critically examine them in terms of their biodiversity, C storage, hydrology and nutrients, paying attention to both above-ground and below-ground subsystems.3. We then propose an approach for better management and restoration of degraded peatlands that involves explicit consideration of multiple interacting ecological factors and the involvement of local communities who rely on converted peatlands for their livelihood. 4. We make the case that as processes leading to peatland development involve modification of both above-ground and below-ground subsystems, an integrated approach that explicitly recognizes both subsystems and their interactions is key to successful tropical peatland management and restoration. Synthesis and applications.Gaining a better understanding of not just carbon stores and their changes during peat degradation, but also an in-depth understanding of the biota, nutrient dynamics, hydrology and biotic and abiotic feedbacks, is key to developing better solutions for the management and restoration of peatlands in Southeast Asia. Through the application of science-and nature-based solutions | 1371

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“…They have been reported in environments such as rice fields, sewers, and freshwater sediments (BRENNER et al, 2005). The community of the acidic peatlands of southern Brazil was investigated and found methanotrophs related to Methylocystaceae and Methylococcaceae (ETTO et al, 2012), as well as in the Amazon region where detected the same families (Methylocystaceae and Methylococcaceae) (FINN et al, 2020;TESSARO, 2012).…”

Section: Microbiological Community Dynamicsmentioning

confidence: 99%

Methanotrophic activity and microbial community dynamics in a UASB sludge

Siniscalchi

Siqueira

Assemany

et al. 2020

RICA

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This study determined the methanotrophic activity in anaerobic sludge from a pilot-scale UASB reactor. Four batch experiments, with three replicates, were performed in 110 mL antibiotic flasks. The results showed that the maximum rate was 115 µmolCH4.d-1 and the methanotrophic activity was 2.3 mmolCH4.gTVS-1.d-1, indicating that the methanotrophic microorganisms play a key role within the UASB reactor since they are part of the sludge microbiota and may consume some of the methane produced inside the reactor. Therefore, these microorganisms may reduce possible methane losses, either atmospheric and/or dissolved in the treated effluent. The microbial community was investigated by molecular tools (PCR-DGGE) and two DNA sequences related to methanotrophic bacteria, Methylocystis sp. (similarity of 93%) and Methylocaldum sp. (similarity of 98%) to 16S rRNA gene sequences, were detected. The methanotrophic activity and the identification of the community of main microorganisms involved allow the reduction of methane into the atmosphere and contribute to the system’s mass balance between production and consumption.

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Methanogens and Methanotrophs Show Nutrient-Dependent Community Assemblage Patterns Across Tropical Peatlands of the Pastaza-Marañón Basin, Peruvian Amazonia

Cited by 40 publications

References 106 publications

Microbial Communities and Interactions of Nitrogen Oxides With Methanogenesis in Diverse Peatlands of the Amazon Basin

Microbial Communities and Interactions of Nitrogen Oxides With Methanogenesis in Diverse Peatlands of the Amazon Basin

Degradation of Southeast Asian tropical peatlands and integrated strategies for their better management and restoration

Methanotrophic activity and microbial community dynamics in a UASB sludge

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